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천화분 추출물이 혈관신생 및 암세포성장에 미치는 영향

Effects of Trichosanthes kirilowii Extract against Angiogenesis and Various Tumor Cells' Growth

손창규|조종관|조정효|이종훈|이연월|유화승|김동우
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2008.06|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제29권 제2호|pp. 490-499

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참고문헌

키워드

CancerAnti-angiogenesisTrichosanthes kirilowii Extract(TKE)
주제분류한의학

초록

Objectives : This study was aimed to elucidate the effects of Trichosanthes kirilowii extract (TKE) on the angiogenesis and growth of tumor cells.Methods : Tube formation assay was performed by using human umbilical vein endothelial cells (HUVEC), and anchorage dependent colony assay was performed by using B16-F10 melanoma, Hep G2 and HT1080, CT-26 and SNU-1 cells.Results : For HUVEC, TKE at a level of more than 100 ㎍/㎖ suppresses cell growth. For HUVEC at 100 ㎍/㎖ and greater TKE density, the formation of tubes was suppressed in a dose-dependant manner. TKE controls the colony formations of B16-F10 melanoma cells, CT 26 cells, and Hep G2 cells, and its effect is proportional to density. In HT1080 cells and SNU-1 cells, formation is suppressed regardless of density.Conclusions : From these results, it could be concluded that TKE has significant properties on anti-angiogenesis and growth inhibiting of tumor cells. It is suggested that TKE will be a good candidate for new drugs or therapeutics for anti-angiogenesis.

Abstract

Objectives : This study was aimed to elucidate the effects of Trichosanthes kirilowii extract (TKE) on the angiogenesis and growth of tumor cells.Methods : Tube formation assay was performed by using human umbilical vein endothelial cells (HUVEC), and anchorage dependent colony assay was performed by using B16-F10 melanoma, Hep G2 and HT1080, CT-26 and SNU-1 cells.Results : For HUVEC, TKE at a level of more than 100 ㎍/㎖ suppresses cell growth. For HUVEC at 100 ㎍/㎖ and greater TKE density, the formation of tubes was suppressed in a dose-dependant manner. TKE controls the colony formations of B16-F10 melanoma cells, CT 26 cells, and Hep G2 cells, and its effect is proportional to density. In HT1080 cells and SNU-1 cells, formation is suppressed regardless of density.Conclusions : From these results, it could be concluded that TKE has significant properties on anti-angiogenesis and growth inhibiting of tumor cells. It is suggested that TKE will be a good candidate for new drugs or therapeutics for anti-angiogenesis.

참고문헌 (32)

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