실고사리(Lygodium japonicum (Thunb.) Sw.) 포자발아와 전엽체 발달조건
Spore Germination and Prothallium Development Conditions of Lygodium japonicum (Thunb.) Sw.
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
본 연구는 실고사리 포자를 이용한 효율적인 증식방법을 마련하기 위해 배지종류, 배양온도, 광질 조건 등 배양환경을 규명하고자 수행되었다. 포자 발아율과 전엽체 발달은 무기물 함량이 낮은 Knop 배지와 1/8MS, 1/4MS 배지에서 74% 이상으로높고, 심장형 전엽체로 발달되었다. 그러나 무기물 함량이 가장적은 Knop 배지에서는 전엽체 노화가 빠르게 진행되었고1/4MS 배지에서는 전엽체 증식이 다소 느렸다. 온도에 따른 발아율은 배양온도가 증가할수록 높아져 30℃에서 86.7%로 가장우수하였으나, 25℃에 비해 전엽체가 얇고, 가근이 비정상적으로 발달되어 실고사리 포자의 발아적온은 25℃로 규명되었다. 광질에 의한 발아율은 LED red에서 90.6%로 형광등(77.2%) 과 LED blue (5.4%)에 비해 높았고, 심장형 전엽체로 발달이진행되었으나 파종 15일 후에는 전엽체 발달이 감소하고 길어졌다. 반면 형광등에서는 정상적인 심장형 전엽체 발달이 진행되었다.
Abstract
This study was conducted to determine the optimal conditions of growth medium, temperature, and light quality for efficient propagation of Lygodium japonicum spores. The rate of spore germination and prothalium development was high in Knop and 1/8MS and 1/4MS media, which had low mineral content; in particular, the germination rate exceeded 74%, and the germinated spores developed into heart-shaped prothallia. However, in Knop‘s medium with the lowest mineral content, a rapid prothallium senescence was observed; in 1/4MS medium, prothallium development was delayed. Germination rate increased with the increase in temperature and reached its maximum, 86.7%, at 30°C; however, at this temperature, the prothallia were thinner and abnormal development of rhizoids was observed compared to normally developed prothallia and rhizoids at 25°C. Therefore, the results suggested that the optimal temperature for L. japonicum spore germination was 25°C. The rate of germination was also measured under different light conditions, and the highest rate of 90.6% was observed under LED red light compared to fluorescent (77.2%) or LED blue (5.4%) lights. The germinated spores developed into heart-shaped prothallia under LED red light; however, 15 days after seeding, prothallium development decreased and the became elongated. In contrast, a normal and continuous development of heart-shaped prothallia was observed under fluorescent light. Key words - Fern, Gametophyte, LED, Propagation, Prothallus
참고문헌 (54)
- Ahn, D. K., "Illustrated Book of Korean Medical Herbs" (2003), Gyohaksa
- Bhardwaja, T. N., "Effect of temperature on the viability of spores of the water fern Marsilea", Sci. Cult, 32, pp.47~48 (1966)
- Bertrand, A. M., "In vitro organogenesis of Polypodium cambricum", Plant Cell Tiss. Org. Cult, 57, pp.65~69 (1999)
- Chang, H. C., "In vitro culture of Drynaria fortunei, a fern species source of Chinese medicine “Gu-Sui-Bu”", In Vitro Cell. Dev. Biol. Plant, 43, pp.133~139 (2007)
- Cox, J., "In vitro spore germination of the fern Schizaea", Sci. Hortic, 97, pp.369~378 (2003)
- Fernandez, H., "In vitro regeneration of Asplenium nidus L. from gametophytic and sporophytic tissue", Sci. Hort, 56, pp.71~77 (1993)
- Fernandez, H., "Influence of tissue culture conditions on apogamy in Dropteris affinis sp. affinis", Plant Cell Tiss. Org. Cult, 45, pp.93~97 (1996)
- Fernandez, H., "Gemmation in Osmunda regalis L. gametophyte culture in vitro", Plant Cell Rep, 16, pp.358~362 (1997)
- Fernandez, H., "Plantlet regeneration in Asplenium nidus L. and Pteris ensiformis L. by homogenization of BA treated rhizomes", Sci. Hort, 68, pp.234~247 (1997)
- Fernandez, H., "Biological and nutritional aspects in fern multiplication", Plant Cell Tiss. Org. Cult, 56, pp.211~214 (1999)
- Fernandez, H., "Gametophyte culture in vitro and antheridiogen activity in Blenchum spicant", Org. Cult, 50, pp.71~74 (1997)
- Fernandez, H., "In vitro culture of ornamental ferns", Plant Cell Tiss. Org. Cult, 73, pp.1~13 (2003)
- Furuya, M., "Photocontrol of spore germination and elementary processes of development in fern gametophytes", Proc. R. Soc. Edinb. B, 86, pp.13~19 (1985)
- Galán, J. M. G., "Working with Ferns: issues and applications", pp.193~205 (2011), Springer
- Galán, J. M. G., "Effect of temperature and dark pretreatment on the germination of three species of Jamesonia(Pteridaceae, Polypodiopsida)", Plant Species Biology, 26, pp.254~258 (2011)
- Haufler, C. H., "Antheridiogen, dark spore germination, and outcrossing mechanisms in Bommeria(Adiantaceae)", American J. Bot, 81, pp.616~621 (1994)
- Higuchi, H., "Effect of 6-benzylaminopurine on the organogenesis of Asplenium nidus L. through in vitro propagation", Sci. Hort, 37, pp.351~359 (1989)
- Higuchi, H., "In vitro propagation of Nephrolepis cordifolia Prsel", Sci. Hort, 32, pp.105~113 (1987)
- 정진아, "꼬리고사리과(Aspleniaceae) 양치식물 3종 전엽체의 기내배양에 미치는 배지구성물질의 영향", 한국자원식물학회지, 19(2), pp.259~12 (2006), 한국자원식물학회
- 정진아, "식용 및 약용 양치식물에서 부위별 추출물의 항산화활성 탐색", 한국자원식물학회지, 20(2), pp.185~192 (2007), 한국자원식물학회
- 강우창, "한국 양치식물의 생활형 구성 분석", 한국자원식물학회지, 25(4), pp.433~446 (2012), 한국자원식물학회[DOI]
- 김나래, "자생 양치식물 9종의 성엽 및 근경 추출물의 α-glucosidase 억제 활성", 한국자원식물학회지, 26(4), pp.411~416 (2013), 한국자원식물학회[DOI]
- Knop, W., "Quantitative untersuchungen uber die ernahrungsprozesse der pflanzen", Landwirsch Vers Stn, 7, pp.93~107 (1865)
- Knudson, L., "A nutrient solution for the germination of orchid seed", Bull. American Orchid Soc, 15, pp.214~217 (1946)
- Kor. Fern Soc. (KFS)., "Illustrated Fern Native to Korea" (2005), Geobook
- Lee, C. H., "Propagation and technique of masspropagation of Pteridophyta native to Korea", Korean Wild Res. Assn, 3, pp.91~96 (2004)
- Lee, C. H., "Masspropagation of Pteris multifida by spore culture in vitro", Korean J. Hort. Sci. Technol, 17, pp.272~ (1999)
- Lee, D. H., "The effect of Lygodium japonicum on experimental rat model of benign prostatic hyperplasia", MS, pp.2010
- Li, S., "Bencao-gangmu" (1992), Orient Publishing Co. Ltd.
- Menendez, V., "Working with Ferns: issues and applications", pp.97~110 (2011), Springer
- Subat-Dezulovic, M., "Drug-induced acute tubulointerstitial nephritis : A case with elevated urinary cadmium", Pediatr. Nephrol, 17, pp.382~385 (2002)
- Murashige, T., "A revised medium for rapid growth and bio-assays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
- Nam, K. H., "Edible ferns of Korea", Korean Ferns Soc, 9, pp.23~30 (2005)
- National Institute of Biological Resources, "National list of species of Korea (Vascular Plants)", National Institute of Biological Resources, 633~ (2011)
- Park, M. K., "Flora of Korean Pteridophyta" (1961), Kyohakdoso Co
- Raghavan, V., "Developmental Biology of Fern Gametophytes" (1989), Cambridge University Press
- Shin, S. L., "Several factors affecting in vitro masspropagation of eight fern species", M.S, pp.2007
- 신소림, "고비의 전엽체 기내 대량번식에 미치는 배지조성 및 배양방법", 원예과학기술지, 27(2), pp.299~304 (2009), 한국원예학회
- 신소림, "자생 양치식물 지상부와 근경 메탄올 추출물의 항산화 효과", 한국자원식물학회지, 23(1), pp.38~46 (2010), 한국자원식물학회
- 신소림, "개고사리의 기내 포자체 재생에 미치는 배지구성물질 및 배양방법의 영향", 한국자원식물학회지, 24(2), pp.113~120 (2011), 한국자원식물학회
- 신소림, "고사리 전엽체의 증식에 미치는 배지구성물질과 배양방법의 영향", 한국자원식물학회지, 22(4), pp.337~342 (2009), 한국자원식물학회
- Smith, H., "Physiological and ecological function within the phytochrome family", Annu. Rev. Plant Physiol, 46, pp.289~315 (1995)
- Smith, H., "Phytochromes and light signal perception by plant emerging synthesis", Nature, 407, pp.585~591 (2000)
- Suetsugu, N., "Cryptogam blue-light photoreceptors", Curr. Opin. Plant Biol, 6, pp.91~96 (2003)
- Sun, X., "Deep-sea pollen from the south China sea : pleistocene indicators of east asian monsoon marine", Marine Geology, 201, pp.97~118 (2003)
- Swami, P., "Control of morphogenesis in the gametophyte of a fern by light and growth hormones", Canadian J. Bot, 58, pp.1464~1473 (1980)
- Tang, S., "Jingshizhenglei-beiji-bencao" (1992), Orient Publishing Co. Ltd
- Wada, M., "Intracellular-localization and dichroic orientation of phytochrome in plasma-membrane and or ectoplasm of a centrifuged protonema of fern Adiantum capillus-veneris L", Plant Cell Physiol, 24, pp.1441~1447 (1983)
- Whittier, D. P., "The effect of light and other factors on spore germination in Botrychium dissectum", Canadian J. Bot, 51, pp.1791~1794 (1973)
- Wynne, G. M., "An antheridiogen, 13-hydroxy-GA73 methyl ester(GA109), from the fern Lygodium circinnatum", Phytochem, 47, pp.1177~1182 (1998)
- Ye, W. C., "A new phenolic glycoside from the roots of Lygodium japonicum", Fitoterapia, 78, pp.600~601 (2007)
- Zhang, L. H., "A new steroidal glycoside from Lygodium japonicum", J. China Pharm. Univ, 37, pp.491~493 (2006)
- Zhang, L. H., "Flavonoids from Lygodium japonicum", Biochem. Syst. Ecol, 34, pp.885~886 (2006)
- Zhu, L., "A new ecdysteroside from Lygodium japonicum(Thunb. )Sw", Nat. Product. Res, 63, pp.215~219 (2009)