좀나도히초미(Polystichum braunii (Spenn.) Fée) 포자체 증식을 위한 기내 포자 발아와 전엽체 배양 조건
Conditions of In Vitro Spore Germination and Prothallium Culture for Sporophyte propagation of Polystichum braunii (Spenn.) Fée
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This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation, and seedling growth in Polystichum braunii (Spenn.) Fée. The rate of spore germination and early prothalium development was high in Knop (41.2%), which had low mineral content. The optimal medium for prothallus propagation and sexual organ formation was 2MS medium (2% sucrose). Among the various mixtures of cultivation soil (bedding soil, peat moss, perlite, and decomposed granite), a mixture of bedding soil and decomposed granite at a ratio of 2:1 (v:v) had a positive effect on sporophyte formation (276.3 ea/7.5 m2). The most efficient conditions for promoting the growth of sporophytes were pots filled with only bedding soil.
Abstract
This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation, and seedling growth in Polystichum braunii (Spenn.) Fée. The rate of spore germination and early prothalium development was high in Knop (41.2%), which had low mineral content. The optimal medium for prothallus propagation and sexual organ formation was 2MS medium (2% sucrose). Among the various mixtures of cultivation soil (bedding soil, peat moss, perlite, and decomposed granite), a mixture of bedding soil and decomposed granite at a ratio of 2:1 (v:v) had a positive effect on sporophyte formation (276.3 ea/7.5 m2). The most efficient conditions for promoting the growth of sporophytes were pots filled with only bedding soil.
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