Regeneration of Cryopreserved Pear Shoot Tips Grown in Vitro by Encapsulation-Dehydration
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
The preservation of pear germplasm, like that of other clonal germplasms, is difficult because it requires conservation of whole plants or their tissues. Among the currently available methods for long-term conservation of clonal germplasm, cryopreservation of shoot tips is the most reliable and cost- and space-effective option. Alginate-coated axillary shoot tips from in vitro−grown pear were conserved successfully in liquid nitrogen (LN) following dehydration. Shoot recovery from cryopreserved shoot tips was improved greatly after 8 weeks of cold acclimation, but recovery decreased slightly after then. The highest regeneration rate was observed when in vitro shoot tips were preincubated in MS (Murashige and Skoog) medium with 0.3 M sucrose for 48 h, and when alginate-coated shoot tips were precultured in MS medium with increasing sucrose concentrations (0.5 M and 0.7 M) for 8 and 16 h, respectively. When the encapsulated beads were dehydrated for up to 7 h [25% water content (fresh weight basis)] under laminar flow, the highest regeneration rate was observed in “BaeYun No. 3” (55.7%) and “Whanggeum” (43.3%) after warming from LN. This technique is useful as a practical procedure to cryopreserve plant material that is sensitive to freezing of the surrounding cryoprotectant medium. Therefore, this technique appears to be promising for the cryopreservation of shoot tips from in vitro−grown plantlets of pear germplasm.
Abstract
The preservation of pear germplasm, like that of other clonal germplasms, is difficult because it requires conservation of whole plants or their tissues. Among the currently available methods for long-term conservation of clonal germplasm, cryopreservation of shoot tips is the most reliable and cost- and space-effective option. Alginate-coated axillary shoot tips from in vitro−grown pear were conserved successfully in liquid nitrogen (LN) following dehydration. Shoot recovery from cryopreserved shoot tips was improved greatly after 8 weeks of cold acclimation, but recovery decreased slightly after then. The highest regeneration rate was observed when in vitro shoot tips were preincubated in MS (Murashige and Skoog) medium with 0.3 M sucrose for 48 h, and when alginate-coated shoot tips were precultured in MS medium with increasing sucrose concentrations (0.5 M and 0.7 M) for 8 and 16 h, respectively. When the encapsulated beads were dehydrated for up to 7 h [25% water content (fresh weight basis)] under laminar flow, the highest regeneration rate was observed in “BaeYun No. 3” (55.7%) and “Whanggeum” (43.3%) after warming from LN. This technique is useful as a practical procedure to cryopreserve plant material that is sensitive to freezing of the surrounding cryoprotectant medium. Therefore, this technique appears to be promising for the cryopreservation of shoot tips from in vitro−grown plantlets of pear germplasm.
참고문헌 (24)
- Benson, E. E., "Use of thermal analysis in the evaluation of cryopreservation protocols of Ribes nigrum L. germplasm", CryoLetters, 17, pp.347~362 (1996)
- Dereuddre, J., "Resistance of alginate-coated somatic embryos of carrot to desiccation and freezing in liquid nitrogen: 2. Thermal analysis", CryoLetters, 12, pp.135~148 (1991)
- Fabre, J., "Encapsulation-dehydration:A new approach to cryopreservation of Solanum shoot tips", CryoLetters, 11, pp.413~426 (1990)
- Finkle, B. J., "Cryopreservation of Plant Cells and Organs", pp.75~115 (1985), CRC Press Inc
- Gonzalez-Arnao, M.T., "Cryopreservation of plant germplasm using the encapsulation-dehydration technique: review and case study on sugarcane", CryoLetters, 27, pp.155~168 (2006)
- Gonzalez-Arnao, M. T., "Importance of pregrowth with sucrose and vitrification of sugar cane apices using encapsulation-dehydration", CryoLetters, 17, pp.141~148 (1996)
- Hirai, D., "Cryopreservation of in vitro-grown meristems of strawberry (Fragaria x ananassa Duch.) by encapsulation-vitrification", Euphytica, 101, pp.109~115 (1998)
- Murashige, T., "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
- Niino, T., "Cryopreservation of alginatecoated in vitro-grown shoot tips of apple, pear and mulberry", Plant Sci, 87, pp.199~206 (1992)
- Niino, T., "Cryopreservation of in in vitro-grown shoot tips of apple and pear by vitrification", Plant Cell Tissue Organ Cult, 28, pp.261~266 (1992)
- Paul, H., "Cryopreservation of apple shoot tips following encapsulationdehydration or encapsulation-vitrification", Plant Cell Rep, 19, pp.768~774 (2000)
- Plessis, P., "Resistance to dehydration and to freezing in liquid nitrogen of alginate coated shoot tips of grapevine", C. R. Acad. Sci. Paris Ser. III, 313, pp.373~380 (1991)
- Reed, B.M., "Cryopreservation of in vitrogrown gooseberry and currant meristems", CryoLetters, 16, pp.131~136 (1995)
- Reed, B. M., "The effect of cold hardening and cooling rate on the survival of apical meristems of Vacinium species frozen in liquid nitrogen", CryoLetters, 10, pp.315~322 (1989)
- Reed, B.M., "Survival of in vitro-grown apical meristems of Pyrus following cryopreservation", HortScience, 25, pp.111~113 (1990)
- Shatnawi, M., "Cryopreservation of apices of in vitro plantlets of almond (Prunus ducis Mill)", CryoLetters, 20, pp.13~20 (1999)
- Tahtamouni, R.W., "Preservation at low temperature and cryopreservation in wild pear (Pyrus syriaca)", Advance in Horticultural Science, 13, pp.156~160 (1999)
- Takagi, H, "Cryopreservation of Tropical Germplasm. Current Research Progress and Application", pp.178~193 (2000), JIRCAS
- Thomashow, M.F., "Role of cold-responsive genes in plant freezing tolerance", Plant Physiol, 118, pp.1~7 (1998)
- Uragami, A., "Cryopreservation of dried axillary buds from plantlets of Asparagus officinalis L. grown in vitro", Plant Cell Rep, 9, pp.328~331 (1990)
- Wang, Q. C., "Cryopreservation of grape vine (Vitis spp.) embryogenic cell suspensions by encapsulation-vitrification", Plant Cell Tissue Org. Cult, 77, pp.267~275 (2004)
- Wu, Y., "Cryopreservation of apple dormant buds and shoot tips", CryoLetters, 22, pp.375~380 (2001)
- Yelenosky, G., "Freezing tolerance of citrus, spinach, and petunia leaf tissue", Plant Physiol, 89, pp.444~451 (1989)
- 이정윤, "Efficient Micropropagation of Pear Germplasm Using Soot Tips and Nodal Explants", 한국자원식물학회지, 28(6), pp.690~696 (2015), 한국자원식물학회[DOI]