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Efficient Cryopreservation of In Vitro Grown Shoot Tips of Strawberry (Fragaria x ananassa Duch.) Germplasm Using Droplet-Vitrification

이정윤|송재영|이선이|이영이|김행훈|이정로|윤문섭|배진주
한국자원식물학회 (The Plant Resources Society Of Korea)|2021.12|한국자원식물학회지 (Korean Journal of Plant Reources.)|제34권 제6호|pp. 600-607

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참고문헌

키워드

StrawberryCryopreservationShoot tipDroplet-vitrification

초록

Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).

Abstract

Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).

참고문헌 (29)

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관련 논문AI추천

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