A Successful Regeneration from Shoot Tips of Chrysanthemum morifolium (Ramat.) following Cryopreservation by Droplet-vitrification
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초록
This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoottips of Chrysanthemum morifolium (Ramat.) cvs. ‘Borami’ and ‘Yes morning’. The shoot tips of Chrysanthemum wereprecultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7 M). Precultured explantswere treated with loading solution (LS, C6) containing glycerol 20% and sucrose 20% for 30 min and exposed todehydration solution (B5) containing 40% of glycerol and 40% of sucrose for 60 min at 25℃, and then transferred ontodroplets containing 2.5 ㎕ PVS3 on sterilized aluminum foils (4 ㎝ × 0.5 ㎝) prior to direct immersion in liquid nitrogen(LN) for 1 h. The highest regeneration rate (%) was obtained when shoot tips were precultured with treatment-2 (exposingof shoot tips to MS + 0.3M sucrose for 30 h and then treated with MS+0.5 M sucrose for 16 h) at 25℃ in both the cultivars. The viability of cooled samples, followed by culturing on NH4NO3-free MS medium for first 5 days was increased totwo-fold (80.7%) regrowth rate over those cultured on normal MS medium or MS medium containing plant growthregulators. This result shows droplet-vitrification would be a promising method for cryobanking chrysanthemumgermplasm.
Abstract
This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoottips of Chrysanthemum morifolium (Ramat.) cvs. ‘Borami’ and ‘Yes morning’. The shoot tips of Chrysanthemum wereprecultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7 M). Precultured explantswere treated with loading solution (LS, C6) containing glycerol 20% and sucrose 20% for 30 min and exposed todehydration solution (B5) containing 40% of glycerol and 40% of sucrose for 60 min at 25℃, and then transferred ontodroplets containing 2.5 ㎕ PVS3 on sterilized aluminum foils (4 ㎝ × 0.5 ㎝) prior to direct immersion in liquid nitrogen(LN) for 1 h. The highest regeneration rate (%) was obtained when shoot tips were precultured with treatment-2 (exposingof shoot tips to MS + 0.3M sucrose for 30 h and then treated with MS+0.5 M sucrose for 16 h) at 25℃ in both the cultivars. The viability of cooled samples, followed by culturing on NH4NO3-free MS medium for first 5 days was increased totwo-fold (80.7%) regrowth rate over those cultured on normal MS medium or MS medium containing plant growthregulators. This result shows droplet-vitrification would be a promising method for cryobanking chrysanthemumgermplasm.
참고문헌 (34)
- Chen, X.L., "Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification", South Afri. J. Bot, 77, pp.397~403 (2011)
- De Boucaud, M.T., "Biotechnology in Agriculture and Forestry 50" (2002)
- Decruse, S.W., "Ammonium nitrate in the culture medium influences regeneration potential of cryopreserved shoot tips of Holostemma annulare", Cryo Lett, 23, pp.55~60 (2002)
- Fukai, S., "Cryopreservation of chrysanthemum shoot tips", Sci. Hortic, 45, pp.167~174 (1990)
- Fukai, S., "Cryopreservation of shoot tips of Chrysanthemum morifolium and related species native to Japan", Euphytica, 54, pp.201~204 (1991)
- Halmagyi, A., "Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches", Plant Cell Rep, 22, pp.371~375 (2004)
- Hitmi, A., "Cryopreservation of Chrysanthemum cinerariaefolium shoot tips", J Plant Physiol, 156, pp.408~412 (2000)
- Khaing, A.A., "Phylogenetic relationships of chrysanthemums in Korea based on novel SSR markers", Gen. Mol. Res, 12, pp.5335~5347 (2013)
- Kim, H.H., "Development of alternative loading solutions in droplet-vitrification procedures", Cryo Lett, 30, pp.291~299 (2009)
- Kim, H.H., "Development of alternative plant vitrification solutions in droplet-vitrification procedures", Cryo Lett, 30, pp.320~334 (2009)
- Kim, H.H., "Cryobanking of Korean Allium germplasm collections: results from 10-yearexperience", Cryoletter, 33, pp.45~57 (2012)
- Kim, H.H., "Cryopreservation of potato cultivated varieties and wild species: critical factors in droplet vitrification", Cryoletter, 27, pp.223~234 (2006)
- Kim, H.H., "Development of cryopreservation protocols through droplet-vitrification and its application to vegetatively propagated crop germplasm", 2010 Annual Conference of The Plant Resources Society of Korea, pp.12~ (2010)
- Kulus, D., "Cryopreservation as a tool used in long-term storage of ornamental species – a review", Sci. Hortic, 168, pp.88~107 (2014)
- Kuriyama, A., "Inhibitory effect of ammonium ion on recovery of cryopreserved rice cells", Plant Sci, 64, pp.231~235 (1989)
- 이윤걸, "작은방울-유리화법에 의한 국화 신초의 초저온동결보존", Korean Journal of Agricultural Science, 38(2), pp.227~233 (2011), 농업과학연구소
- Lee, Y.G., "Improved cryopreservation of chrysanthemum (Chrysanthemum morifolium) using droplet-vitrification", Cryoletter, 32, pp.487~497 (2011)
- Liang, F., "An arabinogalactan from flowers of Chrysanthemum morifolium: structural and bioactivity studies", Carbohydr. Res, 387, pp.37~41 (2014)
- Luyen, B.T., "Anti-inflammatory components of Chrysanthemum indicum flowers", Bioorganic & Med. Chem. Lett, 25, pp.266~269 (2015)
- Martín, C., "Genetic stability analysis of Chrysanthemum (Chrysanthemum × morifolium Ramat.) after different stages of an encapsulation-dehydration cryopreservation protocol", J. Plant Physiol, 168, pp.158~166 (2011)
- Martín, C., "Survival and genetic stability of Dendranthema grandiflora Tzvelev shoot apices after cryopreservation by vitrification and encapsulation-dehydration", Cryobiology, 51, pp.281~289 (2005)
- Martín, C., "Cryopreservation and genetic stability of Dendranthema grandiflora Tzvelev in vitro cultures", Agric. Food Sci, 18, pp.129~135 (2009)
- Murashige, T, "A revised medium for rapid growth and bioassay with tobacco tissue cultures", Physiology Plant, 15, pp.473~497 (1962)
- Panis, B., "Droplet-vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae", Plant Sci, 168, pp.45~55 (2005)
- Sakai, A, "Vitrification, encapsulationvitrification and droplet-vitrification: a review", Cryoletter, 28, pp.151~172 (2007)
- Schwinn, K.E., "Floral flavonoids and their potential for pelargonidin biosynthesis in commercial Chrysanthemum cultivars", Phytochemistry, 35, pp.145~150 (1994)
- Suzuki, M., "A novel preculture method for induction of desiccation tolerance in gentian axillary buds for cryopreservation", Plant Sci, 135, pp.69~76 (1998)
- Teixeira da Silva, J.A., "Ornamental chrysanthemums: improvement by biotechnology", Plant Cell Tiss. Org. Cult, 79, pp.1~18 (2004)
- Teixeira da Silva, J.A., "Chrysanthemum low-temperature storage and cryopreservation: a review", Plant Cell Tiss Organ Cult, 120, pp.423~440 (2015)
- Vidal, N., "In Vitro Cell", Develop. Biol. Plant, 41, pp.63~68 (2005)
- Wang, R.R., "Shoot recovery and genetic integrity of Chrysanthemum morifolium shoot tips following cryopreservation by droplet-vitrification", Sci. Hortic, 176, pp.330~339 (2014)
- Yeung, H.C., "Handbook of Chinese Herbs" (1983), Institute of Chinese Medicine
- 이정윤, "Efficient Cryopreservation of Lilium spp. Shoot Tips using Droplet-vitrification", Plant Breeding and Biotechnology, 1(2), pp.131~136 (2013), 한국육종학회[DOI]
- 이정윤, "Regeneration of Cryopreserved Pear Shoot Tips Grown in Vitro by Encapsulation-Dehydration", 한국자원식물학회지, 30(6), pp.612~617 (2017), 한국자원식물학회[DOI]