PCR-RAPD와 ITS 서열 분석에 의한 두릅나무과(Araliaceae) 의 유연관계 분석
A Phylogenetic Relationships of Araliaceae Based on PCR-RAPD and ITS Sequences
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Abstract
Phylogenetic relationships among species in Araliaceae were analyzed using PCR-RAPDand sequence of ITS region of nuclear ribosomal DNA based on samples collected in Korea.RAPD analysis showed various polymorphic bands which were able to differentiate speciesand genus, and specific bands showing variations among individuals within species. Clusteranalysis using gel images revealed high molecular variability within species of Aralia eleta.No significant variation was found among cultivated species of Panax ginseng, but theyshowed high genetic differences with wild type of the species. In ITS analysis, specificsequences for each genus and species were observed and these were allowed to differentiatespecies and genus. Phylogenetic analysis using ITS sequences showed that Acanthopanaxand Kalopanax had a close relationship, and Araliaand Panax are monophyletic, but genusHederais different species from other species in family Araliaceaein this study. The resultsshowing close relationship between genera Araliaand Panax were also observed in RAPDanalysis. Contrary to the results of RAPD analysis of Panax ginseng, sequence analysis ofITS showed no significant difference between wild mountain ginseng and cultivated speciesof P. ginseng. Also, both RAPD and ITS analysis of P. ginsengshowed no significant geneticvariability among cultivation sites. Results indicate that P. ginsengcultivating in Korea ismonophyletic. The molecular analysis used in this study agreed on classification usingmorphological feature. These results suggest that molecular techniques used in this studycould be useful for phylogenetic analysis of Araliaceae.
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