더덕에서 Aluminum Induced Protein (ClAIP) 유전자의 분리 및 발현분석
Isolation and Expression of Aluminum Induced Protein (ClAIP) Gene from Codonopsis lanceolata
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Abstract
A cDNA clone (GenBank accession no. CF924621) homologous to aluminum inducedprotein gene was isolated and characterized from Codonopsis lanceolata(ClAIP). The ClAIPis 906 nucleotides long and has an open reading frame of 711 bp with a deduced amino acidsequence of 236 residues. The ClAIPshows high homology to A. marina(84%), G. hirsutum(83%), V. radiata(83%), A. thaliana(80%), B. napus(78%) and T. aestivum(68%). Thededuced amino acid sequence of ClAIPalso has homology to the N-terminal end of plant Asnsynthetase. This region does not contain the active sites of the enzyme and the significance ofthis conservation is currently not clear. To investigate the expression of ClAIP againstseveral heavy metal stresses, we treated the sliced tap root of C. lanceolatawith variousheavy metals. The expression of ClAIPwas increased by 25 uM Al2(SO3)4 in proportion toincubation time and also increased by 50 uM CdCl2.
참고문헌 (18)
- "Isolation of human cDNAs for asparagine synthetase and expression in Jensen rat sarcoma cells", pp.2435~2443 (1987)
- "Effect of aluminium on lipid peroxida-tion catalase and peroxidase activities in root tips of soybean", pp.463~468 (1991), Glycine max
- "The effect of aluminium and some other trivalent metal cations on cell division in the root apices of Allium cepa", pp.309~315 (1965)
- "A protein similar to PR proteins is elicited by metal toxicity in wheat roots", pp.211~219 (1993)
- "Isolation and characterisation of a cDNA clone for a harvest induced asparagine synthetase from Asparagus officinalis L", pp.1337~1340 (1993)
- "Aluminum tolerance in wheat analysis of polypeptides in the root apices of tolerant and sensitive genotypes Plant-Soil Interactions at Low pH"
- "Nucleotide sequence of a cDNA for GDP dissociation inhibitor (GDI) which is induced by aluminum (Al) ion stress in tobacco cell culture (accession no. AF012823) (PGR 97.133)", 115, pp.314~ (1997)
- "Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells: possible involvement of peroxidase isozymes in aluminum ion stress", 96, pp.21~28 (1996)
- "Cloning and sequencing of the cDNAs induced by aluminium treatment and Pi starvation in cultured tobacco cells", 93, pp.11~ (1995)
- "Isolation and characterization of wheat aluminum-regulated genes possible involvement of aluminum as a pathogenesis response elicitor", 205, pp.531~538 (1998)
- "Molecular aspects of aluminum toxicity" (1984)
- "Assessing the phytotoxicity of mononuclear hydroxy-aluminum", pp.479~487 (1989)
- "Aluminum induces oxidative stress genes in Arabidopsis thaliana", 116, pp.409~418 (1998)
- "Five genes induced by aluminium in wheat", pp.855~861 (1993)
- "Aluminum induced genes: induction by toxic metals, low calcium, and wounding and pattern of expression in root tips", 107, pp.341~348 (1995)
- "Putative phospholipid hydroperoxide glutathione peroxidase gene from Arabidopsis thaliana induced by oxidative stress", 72, pp.311~316 (1997)
- "Dark-induced and organ specific expression of two asparagine synthetase genes in Pisum sativum", pp.323~332 (1990)
- "Oxidative damage to membranes by a combination of aluminum and iron in suspension-cultured tobacco cells", 38, pp.1333~1339 (1997)