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Molecular Cloning and Characterization of Type 2Metallothionein cDNA from Codonopsis lanceolata(S. et Z.) Trautv

이태후|유창연|Deok Chun Yang|Jun Gyo In|Bum Soo Lee
한국약용작물학회|2005.10|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제13권 제5호|pp. 288-292

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키워드

RT-PCRCodonopsis lanceolataAbiotic stresscadmiummetallothionein

초록

A class I type 2 metallothionein (CMet2) cDNA from taproot of Codonopsis lanceolata was isolated and characterized. A CMet2 cDNA was 572 nucleotides long and had an open reading frame of 234 bp with a deduced amino acid sequence of 78 residues (pI = 4.99). The deduced amino acid sequence of CMet2 matched to the previously reported type 2 metallothionein- like protein genes and showed 74% identity with that of G. max (BAD18377) and C. arietinum (CAA65009). Expression of CMet2 by the RT-PCR was increased at 1 hr after cadmium and hydrogen peroxide treatment, respectively.

Abstract

A class I type 2 metallothionein (CMet2) cDNA from taproot of Codonopsis lanceolata was isolated and characterized. A CMet2 cDNA was 572 nucleotides long and had an open reading frame of 234 bp with a deduced amino acid sequence of 78 residues (pI = 4.99). The deduced amino acid sequence of CMet2 matched to the previously reported type 2 metallothionein- like protein genes and showed 74% identity with that of G. max (BAD18377) and C. arietinum (CAA65009). Expression of CMet2 by the RT-PCR was increased at 1 hr after cadmium and hydrogen peroxide treatment, respectively.

참고문헌 (24)

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