식물류를 이용한 생식용 천연복합식품이 고지방 식이에 의한 당뇨병 유발쥐의 혈청 glucose 함량 및 지질구성에 미치는 영향

Abstract

Taxonomic studies on morphological, principal component analysis (PCA), palynological,RAPD and PCR-RFLP analysis were conducted to intraspecific relationships of Rubusoldhami. Three types of Rubus oldhami based on the flower characters such as petal lengthand number were used in this study. Among the 14 morphological characters, perianthlength, calyx lobe length, apical leaflet shape and leaflet length were used to distinguish foreach type. The pollen characters such as shape, aperture number, surface sculpture wereshowed very similar among three types. Eight primers out of 20 arbitrary primers werescreened for three types, and were revealed 33 (60%) polymorphic bands. The phenogramby RAPD data showed incongruent with morphological analysis. Even though ten restrictionendonucleases produced 20 restriction sites, polymorphic bands were not observed. Basedon the results, three types of Rubus oldhami divided well by morphological characters, butpollen and DNA data were not supported. Therefore, type 1 and 2 which different from type3 by flower characters considered as a temporary hybrid or ecotype because of their similarhabitats.