pCAMBIA 1300 벡터를 이용한 일미벼의 형질전환 및 잎의 전자현미경적 관찰
Transformation of‘ Ilmibyeo’using pCAMBIA 1300 andMicrostructural Investigation of Leaves
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Abstract
- The argEgene of E.coliwas introduced into ‘Ilmibyeo’cultivar of rice by Agrobacterium tumefaciensand alarge number of transgenic plants were produced. Embryogenic calli were co-cultivated with A. tumefaciensstrain AGL1carrying the plasmid pCAMBIA1300 containing hygromycin resistance (HygR). Transgenic plants showing in vitroresistance to 50 mg/L hygromycin were obtained using a selection procedure. Stable integration of argEand HPTgenesinto chromosomal DNA was proven by southern blot analysis and PCR analysis of genomic isolated from T0 progenies.The fragments of 650 bp (HPT) were detected in transgenic rice lines. The 230 bp (argE) fragments were showed inagarose gel, and detected fragments were matched with size of argEspecific primer. The microscopic feature of leaf onscanning electron microscope (SEM) revealed differences between clear and chalky in shape and arrangement of stomabut did not discriminate.Key words - argE, Hygromycin, Ilmibyeo, SEM
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