마 추출물이 방사선처리 식물세포의 생장과 핵 DNA 손상에 미치는 영향
Effects of Yam(Dioscorea batatas Dence) Extracts on the Growth and Nucleus-DNA Damage of the Plant Cells Treated with γ-Radiation
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\식물세포에 마(Dioscorea batatas Dence) 추출액의 전처리가 방사선 스트레스에 노출된 배양세포의 활력, 생장 및 핵 DNA 손상에 미치는 영향을 조사하였다. 마의 분획추출물 중 EtOAc 분획추출물을 식물세포에 전처리하고 20Gy의 방사선에 노출시키면, 마 추출물을 전처리하지 않고 방사선 20Gy만 처리한 세포보다 세포의 활력과 생체중이 20%이상 증가하였다. Comet 분석에서 꼬리부분의 길이(T)와 머리부분의 길이(H)를 측정하여 T/H 비율을 조사하였다. 무처리 세포와 방사선 20Gy를 처리한 세포의 T/H 비율은 각각 1.05 및 1.68로 나타났고, head DNA 량은 각각 86.7% 및 71.3%로 무처리 세포와 방사선을 처리한 세포간에는 큰 차이를 보여, 방사선에 의한 심각한 핵 DNA 손상을 관찰할 수 있었다. 그러나 마 추출물 중 MeOH, EtOAc 및 n-BuOH 분획추출물을 식물세포에 전처리하고 20Gy 방사선을 처리하면, T/H 비율은 각각 1.37, 1.01 및 1.10이었고, head DNA량은 81.5%, 87.6% 및 88.7%로 방사선을 처리 하지 않은 무처리세포 수준으로 회복되었다.
Abstract
\This study was carried out to evaluate the effects of yam(Dioscorea batatas Dence) extracts on the cell viability, growth and nucleus-DNA damage of tobacco cells which were exposed to γ-radiation stress. The viability and growth of tobacco cells exposed to 20Gy of radiation stress were effectively recovered by pretreatment of 10mg/L ethylacetate (EtOAc) yam extract. Pretreatment of EtOAc extract showed 20% higher cell viability and fresh weight growth than that of cells without pretreatment in 20Gy radiation treated tobacco cells. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length(H) in individual comet image isolated from tobacco cells. The T/H ratio of control-cells and treated-cells at 20 Gy were 1.05 and 1.68, and % head DNA of those cell were 86.7 and 71.3%, respectively, suggesting that nuclei of tobacco cells were severely damaged in the integrity of DNA by the treatment of γ-radiation. However, pretreatment of MeOH, EtOAc and n-BuOH extracts decreased radiation induced DNA-damage in the tobacco cells, showing T/H ratio of 1.37, 1.01 and 1.10 and % head DNA of 81.5, 87.6 and 88.7%, respectively.
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