Immunomodulatory Effects of Supplementation with Extracts from the Marine Brown Alga Eisenia bicyclis on Macrophages
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
A large number of edible seaweeds are consumed by the coastal peoples of Asia. Some of them are used in traditional remedies in many parts of the world. In this study we investigated effects of supplementation with ethyl acetate extracts of the brown alga Eisenia bicyclis (EBE) on rat macrophage to evaluate the possibilities as immune-modulators. Twelve male SD rats were divided into two groups and the treatments were as follows: A, no Eisenia bicyclis extract (EBE) intake and distilled water ; B, oral supplemented with EBE 200 mg/kg. After 5 weeks of supplementation, rats were sacrificed to assess the effect on peritoneal macrophage functions. We showed no increasing effects on tumoricidal activity, phagocytic activity and NO production in macrophages in EBE supplementation group. However, EBE supplementation suppressed NO-iNOS production and p65 translocation into the nucleus in LPS-stimulated macrophages. Overall, these results suggest that the supplementation of EBE might have an anti-inflammatory effects on NO-iNOS production in macrophages throughout the inhibition of NF-κB activation.
Abstract
A large number of edible seaweeds are consumed by the coastal peoples of Asia. Some of them are used in traditional remedies in many parts of the world. In this study we investigated effects of supplementation with ethyl acetate extracts of the brown alga Eisenia bicyclis (EBE) on rat macrophage to evaluate the possibilities as immune-modulators. Twelve male SD rats were divided into two groups and the treatments were as follows: A, no Eisenia bicyclis extract (EBE) intake and distilled water ; B, oral supplemented with EBE 200 mg/kg. After 5 weeks of supplementation, rats were sacrificed to assess the effect on peritoneal macrophage functions. We showed no increasing effects on tumoricidal activity, phagocytic activity and NO production in macrophages in EBE supplementation group. However, EBE supplementation suppressed NO-iNOS production and p65 translocation into the nucleus in LPS-stimulated macrophages. Overall, these results suggest that the supplementation of EBE might have an anti-inflammatory effects on NO-iNOS production in macrophages throughout the inhibition of NF-κB activation.
참고문헌 (22)
- Adams, D.O., "The cell biology of macrophage activation", Annu. Rev. Immunol, 2, pp.283~318 (1984)
- Barnes, P.J., "Role of GATA-3 in allergic diseases", Curr. Mol. Med, 8, pp.330~334 (2008)
- Bredt, D.S., "Nitric oxide: a physiologic messenger molecule", Annu. Rev. Biochem, 63, pp.175~195 (1994)
- Ding, A.H., "Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production", J. Immunol, 141, pp.2407~2412 (1988)
- Fidler, I.J., "Therapy of cancer metastasis by systemic activation of macrophages: from the bench to the clinic", Res. Immunol, 144, pp.284~287 (1983)
- Hahn, H., "The role of cell mediated immunity in bacterial infections", Rev. Infect. Dis, 3, pp.1221~1250 (1981)
- Hemmi, H., "The immune response of splenic lymphocytes after cimicifugoside treatment in vitro and pretreatment in vivo", J. Pharmacobiodyn, 3, pp.643~648 (1980)
- Hibbs, J.B. Jr., "Macrophage cytotoxicity: role for L-arginine deaminase and imino nitrogen oxidation to nitrite", Science, 235, pp.473~476 (1987)
- Hibbs, J.B. Jr., "Nitric oxide: a cytotoxic activated macrophage effector molecule", Biochem. Biophys. Res. Commun, 157, pp.87~94 (1998)
- Hu, T., "Antioxidant activity of sulfated polysaccharide fractions extracted from Undaria pinnitafida in vitro", Int. J. Biol. Macromol, 46, pp.193~198 (2010)
- Joe, M.J., "The inhibitory effects of eckol and dieckol from Ecklonia stolonifera on the expression of matrix metalloproteinase-1 in human dermal fibroblasts", Biological and Pharmaceutical Bulletin, 29(8), pp.1735~1739 (2006), Pharmaceutical Society of Japan
- Kang, K., "Antioxidant and antiinflammatory activities of ventol, a phlorotannin-rich natural agent derived from Ecklonia cava, and its effect on proteoglycan degradation in cartilage explant culture", Res. Commun. Mol. Pathol. Pharmacol, 115, pp.77~95 (2004)
- Karin, M., "NF-κB at the crossroads of life and death", Nat. Immunol, 3, pp.221~227 (2002)
- Keller, R., "Abilities of activated macrophages to manifest tumoricidal activity and to generate reactive nitrogen intermediates: a comparative study in vitro and ex vivo", Biochem. Biophys. Res. Commun, 164, pp.968~973 (1989)
- Keller, R., "Lymphokines and bacteria that induce tumoricidal activity, trigger a different secretory response in macrophages", Eur. J. Immunol, 20, pp.695~698 (1990)
- Klimp, A.H., "A potential role of macrophage activation in the treatment of cancer", Crit. Rev. Oncol. Hematol, 44, pp.143~161 (2002)
- Mosmann, T., "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", J. Immunol. Methods, 65, pp.55~63 (1983)
- Nathan, C.F., "Secretory products of macrophages", J. Clin. Invest, 79, pp.319~326 (1987)
- Okimura, T., "Stress and immune responses. III. Effect of resistant stress on delayed type hypersensitivity (DTH) response, natural killer (NK) activity and phagocytosis in mice", Japanese J. Pharmacol, 41, pp.229~235 (1986)
- Speziali, E., "Specific immune responses but not basal functions of B and T cells are impaired in aged mice", Cell. Immunol, 256, pp.1~5 (2009)
- Verstovsek, S., "Tumoricidal activation of murine resident peritoneal macrophages by interleukin-2 and tumor necrosis factor", Cancer Res, 52, pp.3880~3885 (1992)
- Yang, L., "Essential role of nuclear factor- κB in the induction of eosinophilia in allergic airway inflammation", J. Exp. Med, 188, pp.1739~1750 (1998)