Biological Effects of Allium monanthum Extracts on Lipid Metabolism, Anti‐oxidation and the Production of Pro‐inflammatory Cytokines in Rats Fed a High‐Fat Diet
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This study is a basic study on the development of functional substances involved in obesity prevention, lipid metabolism, and immune regulation. Male Sprague‐Dawley rats were fed a high‐fat diet for 10 weeks. Allium monanthum extracts (AME) were administered orally to obesity‐induced rats, and their lipid‐lowering, antioxidative and various types of biological effects related to the immune system were examined. Blood free fatty acid and triglyceride concentrations decreased as the dose of AME increased. Total cholesterol and LDL cholesterol concentrations in the blood decreased as the dose of AME increased. The total cholesterol concentrations in the liver of the AME‐treated groups were lower than the control group. The thiobarbituric acid reactive concentrations were lower in the plasma and liver of all AME‐treated groups than the control group. Plasma AST and ALT activities did not show any significant differences among the treatment groups. IL‐1β and IL‐6 concentrations in the liver tended to decrease as the dose of AME increased. TNF‐α and IL‐10concentrations did now show any significant differences compared to the control group. Lower expression levels of TNF‐α,Apo‐B and Apo‐E genes were found in the AME‐treated groups. Taken together, these results indicate that AME may show positive effects in lipid lowering, antioxidation and anti‐inflammation.
Abstract
This study is a basic study on the development of functional substances involved in obesity prevention, lipid metabolism, and immune regulation. Male Sprague‐Dawley rats were fed a high‐fat diet for 10 weeks. Allium monanthum extracts (AME) were administered orally to obesity‐induced rats, and their lipid‐lowering, antioxidative and various types of biological effects related to the immune system were examined. Blood free fatty acid and triglyceride concentrations decreased as the dose of AME increased. Total cholesterol and LDL cholesterol concentrations in the blood decreased as the dose of AME increased. The total cholesterol concentrations in the liver of the AME‐treated groups were lower than the control group. The thiobarbituric acid reactive concentrations were lower in the plasma and liver of all AME‐treated groups than the control group. Plasma AST and ALT activities did not show any significant differences among the treatment groups. IL‐1β and IL‐6 concentrations in the liver tended to decrease as the dose of AME increased. TNF‐α and IL‐10concentrations did now show any significant differences compared to the control group. Lower expression levels of TNF‐α,Apo‐B and Apo‐E genes were found in the AME‐treated groups. Taken together, these results indicate that AME may show positive effects in lipid lowering, antioxidation and anti‐inflammation.
참고문헌 (34)
- Ahn, E. M., "Development of biologically active compounds from edible plant sources-III. Isolation of flavonoid-glycoside from the Allium monanthum Max", J. Korean Soc. Appl. Biol. Chem, 43, pp.314~316 (2000)
- Baek, N. I., "Development of biologically active compounds from edible plant sources-IV. Isolation of galactosyldiglyceride from the Allium monanthum Max", Korean J. Life Science, 11, pp.93~96 (2011)
- Baker, J., "Role of insulin-like growth factors in embryonic and postnatal growth", Cell, 75, pp.73~82 (1993)
- Bray, G. A, "Obesity increase risk for diabetes", Int. J. Obes. Relat. Metab. Disord., 16, pp.S13~S17 (1992)
- Buege, J. A., "Microsomal lipid peroxidation", Methods Enzymol, 52, pp.302~310 (1978)
- Charrière, G., "Preadipocyte conversion to macrophage : evidence of plasticity", J. Biol. Chem, 278, pp.9850~9855 (2003)
- Choi, J. Y., "Effects of wild garlic on serum component of cholesterol fed rats", J. Korean Oil Chemists' Soc, 9, pp.73~79 (1992)
- Considine, R. V., "Serum immunoreactive leptin concentration in normal-weight and obese humans", N. Engl. J. Med, 334, pp.292~295 (1996)
- Cook, D. G., "Creactive protein concentration in children : relationship to adiposity and other cardiovascular risk factors", Atherosclerosis, 149, pp.139~150 (2000)
- Delgado, A. V., "Production of tumor necrosis factor-α, interleukin-1β, interleukin-2 and interleukin-6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides, 37, pp.355~361 (2003)
- Eckel, R. H., "American Heart Association call to action : obesity as a major risk factor for coronary heart disease. AHA Nutrition Committee", Circulation, 97, pp.2099~2100 (1998)
- Flohe, L., "Convenient assasy for superoxide dismutase, In CRC Handbook of Free Radicals and Antioxidants in Biomedicine", pp.287~293 (1998), CRC Press
- Hill, J. O., "Development of dietary obesity in rats : influence of amount and composition of dietary fat", Int. J. Obes. Relat. Metab. Disord, 16, pp.321~333 (1992)
- Hotamisligil, G. S., "Adipose expression of tumor necrosis factor-α : direct role in obesity-linked insulin resistance", Science, 259, pp.87~91 (1993)
- Ishikawa, T., "Effect of tea flavonoid supplementation on the susceptibility of low-density lipoprotein to oxidative modification", Am. J. Clin. Nutr, 66, pp.261~266 (1997)
- 지태정, "알리인 투여 후 혈소판의 방사선 방어기전 연구", 방사선기술과학, 33(3), pp.185~191 (2010), 대한방사선과학회
- Johansson, L. H., "A spectrophotometric method for determination of catalase activity in small tissue samples", Anal. Biochem, 174, pp.331~336 (1988)
- Kang, Y. H., "Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet", J. Korean Soc. Food Nutr, 25, pp.367~373 (1996)
- Kissebah, A. H., "Role of insulin resistance in adipose tissue and liver in the pathogenesis of endogenous hypertriglyceridaemia in man", Diabetologia, 12, pp.563~571 (1976)
- Kono, Y., "Superoxide radical inhibits catalase", J. Biol. Chem, 257, pp.5751~5754 (1982)
- Krauss, R. M., "Obesity : impact on cardiovascular disease", Circulation, 98, pp.1472~1476 (1998)
- Laganiere, S., "Anti-lipoperoxidation action of food restriction", Biochem. Biophys. Res. Comm, 145, pp.1185~1191 (1987)
- Lee, E., "Effects of powdered Siho(Bupleuri radix)on serum and liver lipid composition and antioxidative capacity in rat fed high oxidized fat", Korean J. Nutrition, 33, pp.502~506 (2000)
- 이은, "솔잎분말이 과산화지질을 급여한 흰쥐의 혈장 및 간장의 지질구성과 항산화능에 미치는 영향", 한국식품영양과학회지, 32(6), pp.926~930 (2003), 한국식품영양과학회
- Lee, K. U., "Artificial induction of intravascular lipolysis by lipid-heparin infusion leads to insulin resistance in man", Diabetologia, 31, pp.285~290 (1988)
- Levander, O. A., "Platelet glutathione peroxidase activity as an index of selenium status in rats", J. Nutr, 113, pp.55~63 (1983)
- Mohamed-Ali, V., "Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-α, in vivo", J. Clin. Endocrinol. Metab, 82, pp.4196~4200 (1997)
- Mohamed-Ali, V., "Adipose tissue as an endocrine and paracrine organ", Int. J. Obes. Relat. Metab. Disord, 22, pp.1145~1158 (1998)
- Nielsen, S., "Obesity and cardiovascular disease : is body structure a factor?", Curr. Opin. Lipidol, 8, pp.200~204 (1997)
- Oppenheim, J. J, "Cytokines : past, present and future", Int. J. Hematol, 74, pp.3~8 (2001)
- Pedersen, B. K., "Exercise and cytokines with particular focus on muscle-derived IL-6", Exerc. Immunol. Rev, 7, pp.18~31 (2001)
- 서정화, "혈관 생리 활성에 미치는 alliin의 효능", 생명과학회지, 19(7), pp.976~982 (2009), 한국생명과학회
- Ueda, H., "Growth-depressing and cholesterollowering effects of quillaja and tea saponins in chicks as influenced by diet composition", Anim. Sci. J, 71, pp.393~399 (2000)
- Weigle, D. S, "Appetite and the regulation of body composition", FASEB J, 8, pp.302~310 (1994)