본문 바로가기
KERD
논문KCI 등재

Microsatellite 표지를 이용한 부안지역 소나무 집단의 화분 유동과 교배양식 추정

Estimating the Parameters of Pollen Flow and Mating System in Pinus densiflora Population in Buan, South Korea, Using Microsatellite Markers

박재인|박유진|홍용표|홍경낙|김영미
한국자원식물학회 (The Plant Resources Society Of Korea)|2015.02|한국자원식물학회지 (Korean Journal of Plant Reources.)|제28권 제1호|pp. 101-110|DOI http://dx.doi.org/10.7732/kjpr.2015.28.1.101

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

Genetic diversityPinus densifloraCorrelation of paternityOutcrossing ratePollen dispersal distance

초록

부안지역 소나무 집단의 화분유동과 교배양식 모수를 추정하기 위하여 7개 microsatellite 표지로 모수, 주변 성목 및 종자에 대한 유전변이를 분석하였다. 이형접합도 기대치(He)와 근교계수(F)는 각각 모수에서 0.614과 0.018, 종자에서 0.624과0.087이며, 각 세대간에 차이는 없었다(P ＞ 0.05). MLTR로 추정한 타가교배율(tm)은 0.967이며, 양친간 근연계수(tm-ts)는0.057, 부계상관(rp)은 0.012로 나타났다. 기존에 보고된 소나무의 동위효소 분석 결과에 비하여 타가교배율은 높고 근친교배 및 부계상관은 낮았으나, microsatellite 표지를 이용한 소나무류의 결과들과는 유사하였다. TwoGener로 추정한 최적 화분비산 모델은 유효밀도(d = 220 trees/ha)를 가정한 정규확산모델로 판명되었으며, 평균 화분비산거리(δ )는 11.42 m로 계산되었다. 화분원 유전적 분화(Φft)는 0.021이며, Mental 검증에서모수간 지리적 거리와 화분원의 유전적 분화는 상관성이 없는것으로 나타났다(r = -0.141, P ＞ 0.05). 부안지역 소나무 집단은 대부분의 화분이 가까운 거리에서 공급되지만, 화분수의 유전다양성이 높고 화분원의 유전적 차이가 작은 상태로 추정된다. 이러한 조건에서 완전한 임의교배가 이루어지기 때문에 종자의 유전자형이 다양하며 세대간 유전변이의 감소가 없는 것으로 사료된다.

Abstract

Parameters of mating system and pollen flow of a Pinus densiflora population in Buan, South Korea, wereestimated using seven nuclear microsatellite markers. The expected heterozygosity (He) was 0.614 in mother trees and 0.624in seeds. Fixation index (F) was 0.018 and 0.087 in each generation. There was no significant genetic difference between thegenerations (P > 0.05). From MLTR, the outcrossing rate (tm), the biparental inbreeding (tm-ts), and the correlation ofpaternity (rp) were 0.967, 0.057, and 0.012, respectively. tm was larger but tm-ts and rp were smaller than those of allozymemarkers in Pinus densiflora. These values were similar to those of microsatellite markers in other pine species. The optimalpollen dispersal model from TwoGener was the normal dispersal model with the effective density of 220 trees/ha and itslevel of genetic differentiation in pollen pool structure (Φft) was 0.021. The average radial distance of pollen flow (δ ) wascalculated as 11.42 m, but no correlation between the pairwise-Φft and the geographical distance among mother trees was atMantel test (r = -0.141, P > 0.05). Although the effective pollen dispersal in the population seems to be restricted, theamount of genetic variation might be maintained in each generation without a loss of genetic diversity. It might be becausethe genetic diversity in pollen pool was high but the genetic difference between pollen donors was small under the completerandom mating condition in the Pinus densiflora population in Buan.

참고문헌 (49)

  1. Adams, W.T, "Gene dispersal within forest tree populations", New Forests, 6, pp.217~240 (1992)
  2. Austerlitz, F, "Two-Generation analysis of pollen flow across a landscape. II. relation between Φft, pollen dispersal and inter female distance", Genet. Soc. Am, 157, pp.851~857 (2001)
  3. Austerlitz, F, "Two-Generation analysis of pollen flow across a landscape. Ⅲ. Impact of adult population structure", Genet. Res, pp.271~280 (2001)
  4. Bittencourt, J.V.M, "Pollen movement within a continuous forest of wind-pollinated Araucaria angustifolia, inferred from paternity and TwoGener analysis", Conservation Genetics, 9, pp.855~868 (2007)
  5. Bower, A.D, "Mating system and inbreeding depression in whitebark pine (Pinus albicaulis Engelm.)", Tree Genet. Genomes, 3, pp.379~388 (2007)
  6. Brown, A.H.D, "Enzyme polymorphism in plant populations", Theor. Pop. Biol, 15, pp.1~42 (1979)
  7. Burczyk, J, "Mating patterns and pollen dispersal in a natural knob-cone pine (Pinus attenuata Lemmon.) stand", Heredity, 77, pp.251~260 (1996)
  8. Carneiro, F.S, "Effects of selective logging on the mating system and pollen dispersal of Hymenaea courbaril L. (Leguminosae) in the Eastern Brazilian Amazon as revealed by microsatellite analysis", For. Ecol. Manag, 262, pp.1758~1765 (2011)
  9. Carvers, S, "Optimal sampling strategy for estimation of spatial genetic structure in tree populations", Heredity, 95, pp.281~289 (2005)
  10. Choi, J.W, "A study for plant community structure and management plan of Pinus densiflora Forest in ByeonsanBando National Park", Kor. J. Env. Ecol, 23(5), pp.447~457 (2009)
  11. Cockerham, C.C, "Estimation of gene flow from F-statistics", Evolution, 47, pp.855~863 (1993)
  12. De-Lucas, A.I, "Mating system and pollen gene flow in Mediterranean maritime pine", Heredity, 100, pp.390~399 (2008)
  13. Dow, B.D, "High levels of gene flow in bur oak revealed by paternity analysis using microsatellites", Heredity, 89, pp.62~70 (1998)
  14. Dyer, R.J, "Contemporary pollen movement in shortleaf pine, Pinus echinata Mill", Ph.D, pp.2002
  15. Ennos, R.A, "Effect of population substructuring on estimates of outcrossing rate on plant populations", Heredity, 48, pp.283~292 (1982)
  16. Eriksson, G, "Dynamic gene conservation for uncertain futures", For. Ecol. Manag, 62, pp.15~37 (1993)
  17. Finkeldey, R, "Genetic implications of silvicultural regimes", For. Ecol. Manag, 197, pp.231~244 (2004)
  18. Guan, L, "Isolation and characterization of tetranucleotide microsatellite loci in Pinus massoniana (Pinaceae)", Am. J. Bot, pp.216~217 (2011)
  19. Han, S.U, "Estimation of effective population numbers and sexual asymmetry based on flowering assessment in clonal seed of orchard Pinus densiflora", Korean. J. Breed, 33(1), pp.29~34 (2001)
  20. Han, S.D, "Estimation of mating system parameters on the natural population in Pinus densiflora of Mt. Juwang", Proceedings of J. Kor. For. Soc, pp.315~316 (2004)
  21. 홍용표, "동위효소 표지와 cpSSR 표지를 이용한 설악산 잣나무 집단의 교배양식", 한국임학회지, 102(2), pp.264~271 (2013), 한국임학회
  22. Jump, A.S, "Environmental change and the option value of genetic diversity", Trends in Plant Sci, 14, pp.51~58 (2009)
  23. KFRI, "Textbook for Forest Management Ⅱ", pp.19~ (2006), KFRI
  24. Kim, Y.M, "Mating system of Japanese red pines in seed orchard using DNA markers", J. Korean Plant Res, 25, pp.63~75 (2012)
  25. 이석우, "Estimation of Mating System Parameters in the Natual Population of Pinus densiflora of Anmyun Island, Korea Using Allozyme Markers", 한국임학회지, 92(2), pp.121~128 (2003), 한국임학회
  26. Lewandowski, A, "Mating system and genetic diversity in natural populations of european larch (Larix decidua) and stone pine (Pinus cembra) located at higher elevations", Silvae Genet, 49(3), pp.158~161 (2000)
  27. Lian, C, "Isolation and characterization of microsatellite loci from the Japanese red pine, Pinus densiflora", Mol. Ecol, 9, pp.1186~1188 (2000)
  28. Lian, C, "Outcrossing and paternity analysis of Pinus densiflora (Japanese red pine) by microsatellite polymorphism", Heredity, 87, pp.88~98 (2001)
  29. Liewlaksaneeyanawin, C, "Genetic evaluation of natural and domesticated lodgepole pine populations using molecular markers", Ph.D, pp.2006
  30. Liewlaksaneeyanawin, C, "Single-copy, species-transferable microsatellite markers developed from lobolly pine ESTs", Theor. Appl. Genet, 109, pp.361~369 (2004)
  31. Lynch, M, "Mutation accumulation and the extinction of small populations", American Naturalist, 146, pp.489~518 (1995)
  32. Mitton, J.B, "The dynamic mating systems of conifers", New Forests, 6, pp.197~216 (1992)
  33. 오구균, "변산반도국립공원의 식생분포 및 녹지자연도 사정에 관한 연구", 한국환경생태학회지, 23(2), pp.161~168 (2009), 한국환경생태학회
  34. Ozawa, H, "Genetic diversity of Pinus densiflora pollen flowing over fragmented populations during a mating season", J. For. Res, 17, pp.488~498 (2012)
  35. Peakall, R, "GENALEX 6.41: Genetic analysis in Excel. Population genetic software for teaching and research", Mol. Ecol. Notes, 6, pp.288~295 (2006)
  36. Raijmann, L.N, "Genetic variation and outcrossing rate in relation to population size in Gentiana pneumontane L", Conservation Biology, 8, pp.1014~1026 (1994)
  37. Rieseberg, L.H, "The biological reality of species: gene flow, selection, and collective evolution", Taxon, 50, pp.47~67 (2001)
  38. Ritland, K, "Extensions of models for the estimation of mating systems using n independent loci", Heredity, 88, pp.211~228 (2002)
  39. "Multilocus mating system program MLTR. Version 3.1" (2004)[URL]
  40. Robledo-Arnuncio, J.J, "Pollen movement under alternative silvicultural practices in native populations of Scots pine (Pinus sylvestris L.) in central Spain", For. Ecol. Manag, 197, pp.245~255 (2004)
  41. Robledo-Arnuncio, J.J, "A new method of estimating the pollen dispersal curve independently of effective density", Genet. Soc. Am, 173, pp.1033~1045 (2006)
  42. Salmela, M.J, "Adaptive genetic variation in Scots pine (Pinus sylvestris L.) in Scotland", Ph.D, pp.2011
  43. Shaw, D.W, "A multilocus estimator of mating system parameters in plant populations", Proc. Natl. Acad. Sci, 78(2), pp.1298~1302 (1981)
  44. Smouse, P.E, "Two-generation analysis of pollen flow across a landscape. I. male gamete heterogeneity among females", Evolution, 55(2), pp.260~271 (2001)
  45. Smouse, P.E, "Measuring pollen flow in forest trees: an exposition of alternative approaches", For. Ecol. Manag, 197, pp.21~38 (2004)
  46. Sork, V.L, "Pollen movement in declining populations of California valley oak, Quercus lobata: where have all the fathers gone", Mol. Ecol, 11, pp.1657~1668 (2002)
  47. Waples, R.S, "LDNe: A program for estimating effective population size from data on linkage disequilibrium", Mol. Ecol. Resour, 8, pp.753~756 (2008)
  48. Watanabe, A, "Isolation of microsatellite markers from Pinus densiflora Sieb. et Zucc. using a dual PCR technique", Mol. Ecol. Notes, 6, pp.80~82 (2006)
  49. Young, A.G, "The population genetic consequences of habitat fragmentation for plants", Trends in Ecol. Evol, 11, pp.413~418 (1996)

관련 논문AI추천

DNA 표지를 이용한 채종원내 소나무의 교배양식 분석김영미, 박재인, 안지영, 홍용표한국자원식물학회 · 한국자원식물학회지2012 · 제25권 제1호ESTP 표지를 이용한 국내 소나무 집단의 유전변이강호덕, 안지영, 이제완, 홍경낙, 홍용표한국자원식물학회 · 한국자원식물학회지2015 · 제28권 제2호Analysis of Cone and Seed Characteristics from Different Mating Design Strategies of Pinus densiflora for. multicaulis강준원, 이현석, Kyungmi Lee산림과학연구소 · Journal of forest and environmental science2021 · 제37권 제4호소나무 풍매차대의 생장 특성에 대한 유전 모수의 수령간 변화강길남, 김영중, 김장수, 오창영, 이석면, 한상억한국육종학회 · 한국육종학회지2007 · 제39권 제4호설악산 대청봉 눈잣나무(Pinus pumila (Pall.) Regel) 집단의 유전다양성과 공간적 유전구조송정호, 임효인, 장경환, 홍경낙, 홍용표한국자원식물학회 · 한국자원식물학회지2012 · 제25권 제4호Genetic variation and structure of Juniperus chinensis L. (Cupressaceae) in Korea김은혜, 신재권, 이창석, 정금선, 정재민한국생태학회 · Journal of Ecology and Environment2018 · 제42권 제3호

이 간행물의 다른 논문 (12건)

산약이 흰쥐의 급성 위궤양에 의한 위장 장애에 미치는 효과임재환, 서을원, 박영미pp. 1-8임상에서 분리한 메치실린 내성 황색포도상구균에 대한 녹나무잎 추출물의 항균활성전아림, 황승미, 차정단, 최미래, 강제란, 오좌섭, 박영진, 최경민, 정용환, 고은실pp. 9-15온주밀감 유전자원의 과즙 중 플라보노이드 특성 및 군집 분석김진영, 양영택, 박영철, 강종훈, 강상훈, 이창훈pp. 16-25국내 윤노리나무 6개 집단의 형태적 특징과 변이조사남재익, 김영미, 박재인, 최고은pp. 26-34월악산 국립공원의 관속식물상김기홍, 장현도, 이로영, 장창석, 양선규, 박민수, 오병운pp. 35-63
원문 보기