까마귀쪽나무 열매 추출물의 골관절염 억제 효과
Anti-osteoarthritis Effects on Fruit Extract of Litsea japonica
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본 연구는 까마귀쪽나무 열매 70% 주정에탄올 추출물을 가지고 제작된 인체적용시험시료(LJTM)의 항염증 및 골관절염biomarker의 변화를 통한 관절건강 기능성식품을 개발하기 위하여 인체적용시험시료를 제작한 후 이에 대한 유효성분의 함량을 평가하고, 인체적용시험전에 그 효력이 유지됨을 확인하기 위하여 수행되었다. 본 연구에 사용된 시료 LJTM은 NO생성이 억제되는 농도에서 세포독성이 관찰되지 않았으며, TNF-α 와 IL-6 생성이 농도 의존적으로 억제되고 PGE2를 억제하였다. 또한 동물시험에서 골관절염의 biomarker인 MMP-2, 3, 7, 9 와 TIMP-1, 2에 대한 mRNA 발현이 농도 의존적으로 억제되었으며, 중추신경계 및 말초신경계에 의한 통증을 억제하는 것으로 평가되었다. 따라서 까마귀쪽나무 열매 70% 추출물이 함유된 인체적용시험시료(LJTM)는 골관절염과 진통억제에 우수한효과가 있는 것으로 확인되었다.
Abstract
Osteoarthritis (OA) is a degenerative disease characterized by the progressive degradation of joint cartilage and is accompanied by secondary inflammation of synovial membranes. The purpose of this study describes a preliminary evaluation of the anti-inflammatory activity on test material of Litsea japonica. fruit (LJTM) Also, this study was to evaluate the effects of LJTM on the joint cartilage of rat with OA induced by monosodium iodoacetate (MIA). To study for anti-inflammatory agents effectively, we first examined the inhibitory effect of the LJTM on the production of pro-inflammatory factors and cytokines stimulated with lipopolysaccharide. We identified anti-nociceptive effects of the LJTM by using in vivo peripheral and central nervous pain models. In addition, the aim of this study was to evaluate the effects on mRNA expression of MMP-2, -3, -7, -9, -13, TIMP-1 and –2 in cartilage of OA. In the LJTM inhibited production of pro-inflammatory mediators (NO and PGE2) and pro-inflammatory cytokines (TNF-α and IL-6). In cartilage, Expression of MMPs and TIMPs mRNA was suppressed in LJTM treatment group than in the control group. This study suggests that LJTM are potential candidates as anti-inflammation and anti-osteoarthritis agents (painkillers) for the treatment of OA.
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