본문 바로가기
KERD
논문

LPS로 자극된 RAW 264.7 대식세포에서의 고광나무 메탄올 추출물의 항염증 활성 Anti-inflammatory Activity of Philadelphus schrenkii Methanol Extract in Lipopolysaccharide-stimulated RAW 264.7 Cells

Anti-inflammatory Activity of Philadelphus schrenkii Methanol Extract in Lipopolysaccharide-stimulated RAW 264.7 Cells

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Background: Philadelphus schrenkii Rupr. is a widespread in East Asia and has historically been used as a medicinal herb in Korea to reduce heat, swelling, and pain. This study investigated the anti-inflammatory effects of P. schrenkii methanol extract (PSE) on lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Methods and Results: PSE inhibited the expression of nitric oxide, prostaglandin E2, inducible nitric oxide synthase, and cyclooxygenase-2 at concentrations that did not affect cell viability as determined by the tetrazolium salt (WST-1) assay. The quantitative reverse transcription poly- merase chain reaction and enzyme-linked immunosorbent assay measured that pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and monocyte chemoat- tracted protein-1, were decreased in PSE-treated groups. Western blotting confirmed that PSE inhibited the phosphorylation of nuclear factor kappa B and mitogen-activated protein kinases. Gas chromatography-mass spectrometry analysis revealed the presence of active anti-inflammatory compounds in PSE. Conclusions: This study demonstrated that PSE has potent anti-inflammatory activity in vitro and pro- vides a reasonable basis for the traditional use of P. schrenkii in treating inflammation-related diseases.

Abstract

Background: Philadelphus schrenkii Rupr. is a widespread in East Asia and has historically been used as a medicinal herb in Korea to reduce heat, swelling, and pain. This study investigated the anti-inflammatory effects of P. schrenkii methanol extract (PSE) on lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Methods and Results: PSE inhibited the expression of nitric oxide, prostaglandin E2, inducible nitric oxide synthase, and cyclooxygenase-2 at concentrations that did not affect cell viability as determined by the tetrazolium salt (WST-1) assay. The quantitative reverse transcription poly- merase chain reaction and enzyme-linked immunosorbent assay measured that pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and monocyte chemoat- tracted protein-1, were decreased in PSE-treated groups. Western blotting confirmed that PSE inhibited the phosphorylation of nuclear factor kappa B and mitogen-activated protein kinases. Gas chromatography-mass spectrometry analysis revealed the presence of active anti-inflammatory compounds in PSE. Conclusions: This study demonstrated that PSE has potent anti-inflammatory activity in vitro and pro- vides a reasonable basis for the traditional use of P. schrenkii in treating inflammation-related diseases.

참고문헌 (75)

  1. Al-Marzoqi AH, "Determination of metabolites products by Cassia angustifolia and evaluate antimicrobial activity", Journal of Pharmacognosy and Phytotherapy, 8, pp.25~48 (2016)
  2. Alonso-Castro AJ, "Myristic acid reduces skin inflammation and nociception", Journal of Food Biochemistry, 46, pp.e14013~ (2022)
  3. Ameamsri U, "Oleamide in Ipomoea and Dillenia species and inflammatory activity investigated through ion channel inhibition", Current Pharmaceutical Biotechnology, 22, pp.254~261 (2021)
  4. Aparna V, "Anti-inflammatory property of n-hexadecanoic acid : Structural evidence and kinetic assessment", Chemical Biology and Drug Design, 80, pp.434~439 (2012)
  5. Asanka Sanjeewa K, "Ecklonia cava(Laminariales)and Sargassum horneri(Fucales)synergistically inhibit the lipopoly-saccharide-induced inflammation via blocking NF-κB and MAPK pathways", Algae, 34, pp.45~56 (2019)
  6. Ben Neriah Y, "Inflammation meets cancer, with NF-κB as the matchmaker", Nature Immunology, 12, pp.715~723 (2011)
  7. Cao J, "Dehydroepiandrosterone attenuates LPS-induced inflammatory responses via activation of Nrf2 in RAW264. 7 macrophages", Molecular Immunology, 131, pp.97~111 (2021)
  8. Chakrabarti M, "Investigating the underlying mechanism of cadmium-induced plant adaptive response to genotoxic stress", Ecotoxicology and Environmental Safety, 209, pp.111817~ (2021)
  9. Chang MC, "Antiplatelet effect of catechol is related to inhibition of cyclooxygenase, reactive oxygen species, ERK", p38 signaling and thromboxane A2 production, 2014, pp.9, e104310~
  10. Chen L, "Inflammatory responses and inflammation-associated diseases in organs", Oncotarget, 9, pp.7204~7218 (2018)
  11. Chinnadurai V, "Comparative studies of phytochemical analysis and pharmacological activities of wild and micropropagated plant ethanol extracts of Manihot esculenta", Biocatalysis and Agricultural Biotechnology, 19, pp.101166~ (2019)
  12. Chippada SC, "In vitro Anti-inflammatory activity of methanolic extract of centella asiatica by HRBC membrane stabilisation", Rasayan Journal of Chemistry, 4, pp.457~460 (2011)
  13. Chuang LT, "In vitro and in vivo screening of wild bitter melon leaf for anti-inflammatory activity against Cutibacterium acnes", Molecules, 25, pp.4277~ (2020)
  14. Closse A, "2, 3-Dihydrobenzofuran-2-ones : A new class of highly potent anti-inflammatory agents", Journal of Medicinal Chemistry, 24, pp.1465~1471 (1981)
  15. da Silva RM, "The postprandial inflammatory response is attenuated by a dietary structured lipid containing behenic acid", Journal of Functional Foods, 58, pp.350~354 (2019)
  16. Das U, "Arachidonic acid has anti-inflammatory and anti-diabetic actions in vitro and in vivo", Current Developments in Nutrition, 4, pp.747~747 (2020)
  17. Dinarello CA, "Proinflammatory cytokines", Chest, 118, pp.503~508 (2000)
  18. Dong L, "Anti-inflammatory effects of ononin on lipopolysaccharide-stimulated RAW 264. 7 cells", Molecular Immunology, 83, pp.46~51 (2017)
  19. Funk CD, "Prostaglandins and leukotrienes : Advances in eicosanoid biology", Science, 294, pp.1871~1875 (2001)
  20. Gabay C, "Interleukin-6 and chronic inflammation", Arthritis Research and Therapy, 8, pp.1~6 (2006)
  21. Habib S, "Biochemistry of nitric oxide", Indian Journal of Clinical Biochemistry, 26, pp.3~17 (2011)
  22. Hamed A, "Methyl palmitate : The naturally occurring cardioprotective agent", Archives of Pharmaceutical Sciences Ain Shams University, 4, pp.47~62 (2020)
  23. Holanda Pinto S, "Anti-inflammatory effect of α, β-amyrin, a pentacyclic triterpene from Protium heptaphyllum in rat model of acute periodontitis", Inflammopharmacology, 16, pp.48~52 (2008)
  24. Insuan O, "Antidesma thwaitesianum Müll. Arg. fruit extract rich in 5-hydroxymethylfurfural exhibits anti-inflammatory effects in lipopolysaccharide-stimulated RAW264. 7 macrophages", Journal of Herbmed Pharmacology, 11, pp.278~285 (2022)
  25. Islam MT, "Phytol : A review of biomedical activities", Food and Chemical Toxicology, 121, pp.82~94 (2018)
  26. Jananie R, "Determination of bioactive components of Cynodon dactylon by GC-MS analysis", New York Science Journal, 4, pp.16~20 (2011)
  27. Jayawardena TU, "Loliolide, isolated from Sargassum horneri; abate LPS-induced inflammation via TLR mediated NF-κB, MAPK pathways in macrophages", Algal Research, 56, pp.102297~ (2021)
  28. Kim AL, "Role of p38 MAPK in UVB-induced inflammatory responses in the skin of SKH-1 hairless mice", Journal of Investigative Dermatology, 124, pp.1318~1325 (2005)
  29. Kim BH, "Anti-inflammatory activity of compounds isolated from Astragalus sinicus L. in cytokine-induced keratinocytes and skin", Experimental and Molecular Medicine, 46, pp.e87~ (2014)
  30. Kim EA, "Tuberatolide B isolated from Sargassum macrocarpum inhibited LPS-stimulated inflammatory response via MAPKs and NF-κB signaling pathway in RAW264. 7 cells and zebrafish model", Journal of Functional Foods, 52, pp.109~115 (2019)
  31. Kim EA, "Anti-inflammatory effect of Apo-9′-fucoxanthinone via inhibition of MAPKs and NF-κB signaling pathway in LPS-stimulated RAW 264. 7 macro phages and zebrafish model", International Immunopharmacology, 59, pp.339~346 (2018)
  32. Kim J, "Antioxidant activity of edible sprouts and phytosterol contents by HPLC", UV analysis, 2022, pp.63, 769~778
  33. Kim J, "In vitro Anti-inflammatory activities and phenolic acid analysis of tree sprout extracts", Korean Journal of Pharmacognosy, 52, pp.257~266 (2021)
  34. Konishi I, "Suppressive effects of alloxanthin and diatoxanthin from Halocynthia roretzi on LPS-induced expression of pro-inflammatory genes in RAW264. 7 cells", Journal of Oleo Science, 57, pp.181~189 (2008)
  35. Kumar PP, "Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo", African Journal of Biochemistry Research, 4, pp.191~195 (2010)
  36. Lee CK, "Screening and isolation of antibiotic resistance inhibitors from herb materials-resistance inhibition of volatile components of Korean aromatic herbs", Archives of Pharmacal Research, 21, pp.62~66 (1998)
  37. Lee SM, "Anti-inflammatory effects of cowpea(Vigna sinensis K. )seed extracts and its bioactive compounds", Journal of the Korean Society for Applied Biological Chemistry, 54, pp.710~717 (2011)
  38. Liu X, "LPS-induced proinflammatory cytokine expression in human airway epithelial cells and macrophages via NF-κB, STAT3 or AP-1 activation", Molecular Medicine Reports, 17, pp.5484~5491 (2018)
  39. Marasinghe CK, "Anti- inflammatory action of ark shell(Scapharca subcrenata) protein hydrolysate in LPS-stimulated RAW264.7 murine macrophages", Journal of Food Biochemistry, 46, pp.e14493~ (2022)
  40. Mazimba O, "Umbelliferone: Sources, chemistry and bioactivities review", Bulletin of Faculty of Pharmacy, Cairo University, 55, pp.223~232 (2017)
  41. Miyasaka N, "Nitric oxide and inflammatory arthritides", Life Sciences, 61, pp.2073~2081 (1997)
  42. Mohammed GJ, "Antibacterial and phytochemical analysis of Piper nigrum using gas chromatography-mass spectrum and Fourier-transform infrared spectroscopy", International Journal of Pharmacognosy and Phytochemical Research, 8, pp.977~996 (2016)
  43. Munn LL, "Cancer and inflammation", WIREs Mechanisms of Disease, 9, pp.e1370~ (2017)
  44. Myung DB, "Oral intake of hydrangea serrata(Thunb. )ser. leaves extract improves wrinkles, hydration, elasticity, texture, and roughness in human skin : A randomized, double-blind, placebo-controlled study", Nutrients, 12, pp.1588~ (2020)
  45. Nakamura S, "Search for antidiabetic constituents of medicinal food", Journal of the Pharmaceutical Society of Japan, 131, pp.909~915 (2011)
  46. Newman DJ, "Natural products as sources of new drugs over the nearly four decades from 01", 1981 to 09, Journal of Natural Products, pp.2020 (2019), 83[DOI]
  47. Newton K, "Signaling in innate immunity and inflammation", Cold Spring Harbor Perspectives in Biology, 4, pp.e006049~ (2012)
  48. Nore KG, "Elevated levels of anti-inflammatory eicosanoids and monocyte heterogeneity in Mycobacterium tuberculosis infection and disease", Frontiers in immunology, 11, pp.579849~ (2020)
  49. Nunes CDR, "Plants as sources of anti-inflammatory agents", Molecules, 25, pp.3726~ (2020)
  50. Okur ME, "In vivo wound healing and in vitro anti-inflammatory activity evaluation of Phlomis russeliana extract gel formulations", Molecules, 25, pp.2695~ (2020)
  51. Olukanni A, "Antioxidant and in vitro anti-inflammatory activities of Albizia zygia(DC)JF mebr and the evaluation of its phytochemical constituents", Journal of Medicinal Plants Studies, 8, pp.317~323 (2020)
  52. Omeje K, "Pharmacological potentials, characterization and fatty acids profile of Persea americana Mill. (avocardo)seed oil using gas chromatography-mass spectroscopy", Biochemistry and Analytical Biochemistry, 7, pp.1000361~ (2018)
  53. Park S, "Taxonomic reconsideration of the Philadelphus schrenkii complex", Korean Journal of Plant Taxonomy, 35, pp.247~272 (2005)
  54. Rajeswari G, "GC-MS analysis of bioactive components of Hugonia mystax L.(Linaceae)", Research Journal of Pharmaceutical, Biological and Chemical Sciences, 3, pp.301~308 (2012)
  55. Ramadass V, "Small molecule NF-κB pathway inhibitors in clinic", International Journal of Molecular Sciences, 21, pp.5164~ (2020)
  56. Seo J, "Anti-inflammatory and antioxidant activities of methanol extract of Piper betle Linn. (Piper betle L. )leaves and stems by inhibiting NF-κB", MAPK, Biomedicine and Pharmacotherapy, pp.2022, 155[DOI]
  57. Shalini K, "Preliminary phytochemical studies, GC-MS analysis and in vitro antioxidant activity of selected medicinal plants and its polyherbal formulation", Pharmacognosy Journal, 13, pp.648~659 (2021)
  58. Sharma JN, "Role of nitric oxide in inflammatory diseases", Inflammopharmacology, 15, pp.252~259 (2007)
  59. Shi J, "Monoterpenes from the leaves of Hydrangea paniculata and their hepatoprotective activities", Journal of Asian Natural Products Research, 17, pp.512~518 (2015)
  60. Shin KH, "Antimicrobial activities of volatile essential oils from Korean aromatic plants", Natural Product Sciences, 3, pp.141~147 (1997)
  61. Simon LS, "Role and regulation of cyclooxygenase-2 during inflammation", The American Journal of Medicine, 106, pp.37S~42S (1999)
  62. Smale ST, "Hierarchies of NF-κB target-gene regulation", Nature Immunology, 12, pp.689~694 (2011)
  63. Sung YY, "Anti-obesity effects of Philadelphus tenuifolius extract via improvement of lipid metabolism in high-fat diet-induced obese mice", Planta Medica, 89, pp.273~ (2023)
  64. Taciak B, "Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages", PLOS ONE, 13, pp.e0198943~ (2018)
  65. Tsai FS, "Lupeol and its role in chronic diseases", Drug Discovery from Mother Nature, 2016, pp.145~175 (2016)
  66. Vadivel E, "GC-MS analysis of some bioactive constituents of Mussaenda frondosa Linn", International Journal of Pharma and Bio Sciences, 2, pp.313~320 (2011)
  67. Venn-Watson SK, "Broader and safer clinically-relevant activities of pentadecanoic acid compared to omega-3: Evaluation of an emerging essential fatty acid across twelve primary human cell-based disease systems", PLOS ONE, 17, pp.e0268778~ (2022)
  68. Viatour P, "Phosphorylation of NF-κB and IκB proteins : Implications in cancer and inflammation", Trends in Biochemical Sciences, 30, pp.43~52 (2005)
  69. Vuolteenaho K, "The role of nitric oxide in osteoarthritis", Scandinavian Journal of Rheumatology, 36, pp.247~258 (2007)
  70. Wang J, "Function and inhibition of P38 MAP kinase signaling: Targeting multiple inflammation diseases", Biochemical Pharmacology, pp.115973~ (2023)
  71. Wink DA, "Nitric oxide and redox mechanisms in the immune response", Journal of Leukocyte Biology, 89, pp.873~891 (2011)
  72. Xiao P., "Zhongguo ben cao tu lu(Vol. 7)", pp.68~ (1989), Ren min wei sheng chu ban she
  73. Yadav A, "MCP-1 : Chemoattractant with a role beyond immunity : A review", Clinica Chimica Acta, 411, pp.1570~1579 (2010)
  74. Yang GS, "Inhibition of lipopolysaccharide-induced nitric oxide and prostaglandin E2 production by chloroform fraction of Cudrania tricuspidata in RAW 264. 7 macrophages", BMC Complementary and Alternative Medicine, 12, pp.1~7 (2012)
  75. Zhang D, "LAMC2 regulated by microRNA-125a-5p accelerates the progression of ovarian cancer via activating p38 MAPK signalling", Life Sciences, 232, pp.116648~ (2019)

관련 논문AI추천

이 간행물의 다른 논문 (3건)