Anti-inflammatory Activity of Complex Saponins Isolated from a Puerariae lobata Flower and Phaseolus angularis Mixture Extract
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초록
Puerariae lobata flower and Phaseolus angularis contain several bioactive compounds, including saponins, oleanolic acid, and flavones. Traditionally, P. lobata flower and P. angularis have been used to treat disorders of antioxidant activity, diabetes, and liver detoxication, and they have anti-nociceptive and anti-inflammatory properties. However, the anti-inflammatory activities of complex saponins has not been scientifically investigated. The study aim was to determine the anti-inflammatory activities of the complex saponins extracted from a mixture of P. lobata flower and P. angularis on nitric oxide (NO) and in a prostaglandin E2 assay. The anti-inflammatory activities of the complex saponins extracted from a P. lobata flower and P. angularis mixture extract were evaluated for their inhibitory activities against lipopolysaccharide- induced NO and prostaglandin E2 expression in RAW264.7 cell lines. The extracted complex saponins exhibited inhibitory activity in both tests, with high percentage depression values in the range of 50–100 μg/ml. Inhibition of inflammation was higher in the prostaglandin E2 tests than in the NO tests. These results suggest that the complex saponins extracted from a mixture of P. lobata flower and P. angularis could be used as an anti-inflammatory agent.
Abstract
Puerariae lobata flower and Phaseolus angularis contain several bioactive compounds, including saponins, oleanolic acid, and flavones. Traditionally, P. lobata flower and P. angularis have been used to treat disorders of antioxidant activity, diabetes, and liver detoxication, and they have anti-nociceptive and anti-inflammatory properties. However, the anti-inflammatory activities of complex saponins has not been scientifically investigated. The study aim was to determine the anti-inflammatory activities of the complex saponins extracted from a mixture of P. lobata flower and P. angularis on nitric oxide (NO) and in a prostaglandin E2 assay. The anti-inflammatory activities of the complex saponins extracted from a P. lobata flower and P. angularis mixture extract were evaluated for their inhibitory activities against lipopolysaccharide- induced NO and prostaglandin E2 expression in RAW264.7 cell lines. The extracted complex saponins exhibited inhibitory activity in both tests, with high percentage depression values in the range of 50–100 μg/ml. Inhibition of inflammation was higher in the prostaglandin E2 tests than in the NO tests. These results suggest that the complex saponins extracted from a mixture of P. lobata flower and P. angularis could be used as an anti-inflammatory agent.
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