둥근마 에탄올 추출물의 항염증 효과
Anti-inflammatory Effect of Ethanol Extracts from Dioscorea bulbifera
0
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
Dioscorea bulbifera has been cultivated in Korea, China and Japan as a food, and also widely used as a traditional medicine for a long time. This study was conducted to determine the anti-inflammatory effect of Dioscorea bulbifera ethanol extracts. The inhibitory effect of Dioscorea bulbifera ethanol extracts were evaluated regarding production or expression of inflammatory mediators in macrophage cells. Dioscorea bulbifera ethanol extracts significantly inhibited the expression of IL-4, IFN-γ and PGE2 and expression of COX-2, iNOS, TNF-α, in LPS-stimulated RAW264.7 cells. In addition, we evaluated the inhibitory effect of Dioscorea bulbifera ethanol extracts on an atopic dermatitis-like mouse model. An atopic dermatitis-like skin lesion was induced by repeated treatment of DNCB on the ear skin of ICR mice. The efficacy of Dioscorea bulbifera ethanol extracts was tested by histophathologic analysis. Histophathologic analysis revealed a reduction in the ear thickness in Dioscorea bulbifera ethanol extracts treated mice. These results show that Dioscorea bulbifera ethanol extracts have an anti-inflammatory effect. Therefore, this research suggests that Dioscorea bulbifera may be a potential agent for ameliorating inflammation.
Abstract
Dioscorea bulbifera has been cultivated in Korea, China and Japan as a food, and also widely used as a traditional medicine for a long time. This study was conducted to determine the anti-inflammatory effect of Dioscorea bulbifera ethanol extracts. The inhibitory effect of Dioscorea bulbifera ethanol extracts were evaluated regarding production or expression of inflammatory mediators in macrophage cells. Dioscorea bulbifera ethanol extracts significantly inhibited the expression of IL-4, IFN-γ and PGE2 and expression of COX-2, iNOS, TNF-α, in LPS-stimulated RAW264.7 cells. In addition, we evaluated the inhibitory effect of Dioscorea bulbifera ethanol extracts on an atopic dermatitis-like mouse model. An atopic dermatitis-like skin lesion was induced by repeated treatment of DNCB on the ear skin of ICR mice. The efficacy of Dioscorea bulbifera ethanol extracts was tested by histophathologic analysis. Histophathologic analysis revealed a reduction in the ear thickness in Dioscorea bulbifera ethanol extracts treated mice. These results show that Dioscorea bulbifera ethanol extracts have an anti-inflammatory effect. Therefore, this research suggests that Dioscorea bulbifera may be a potential agent for ameliorating inflammation.
참고문헌 (33)
- Pratt D. E., "Phenilic Compounds in Food and Their Effects on Health(Ⅱ)", pp.57~71 (1992), Amesican Chemical Society
- 안정희, "부채마 (Dioscorea nipponica Makino)의 부정근 배양조건과 Steroidal Saponin의 기내생산", Journal of Plant Biotechnology, 32(3), pp.217~223 (2005), 한국식물생명공학회
- 김지인, "마(Dioscorea batatas Decne)의 항균, 항산화 및 항혈전 활성 평가", 한국미생물·생명공학회지, 37(2), pp.133~139 (2009), 한국미생물·생명공학회
- 김대성, "마 에탄올추출물의 피부 collagen 합성 촉진 및 MMPs 활성 억제효과", 동의생리병리학회지, 27(2), pp.183~188 (2013), 한의병리학회
- Development of functional new materials from some korean medical plants, "Kim H. K.", Korea Food Research Institute, ~, pp.E1218-0420 (1993)
- Goktepe I., "Probiotics in food safety and human health(Food Biorechnol 6)", pp.12~19 (2006), Taylor & Fracis
- Wang L., "Diosgenin inhibits IL-1β-induced expression of inflammatory mediators in human osteoarthritis chondrocytes", International Journal of Clinical and Experimental Pathology, 8(5), pp.4830~4836 (2015)
- Lin Y., "Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways", Free Radical Research, 48(12), pp.1485~1493 (2014)
- 오혜숙, "산수유 열수추출물을 첨가한 찜류용 기능성 간장소스 개발", 한국식생활문화학회지, 21(5), pp.550~558 (2006), 한국식생활문화학회
- Lee M. Y., "Protective effects of allantoin against ovalbumin (OVA)-induced lung inflammation in a murine model of asthma", International Immunopharmacology, 10(4), pp.474~480 (2010)
- Jang M, "Extraction Optimization for Obtaining Artemisia capillaris Extract with High Anti-Inflammatory Activity in RAW 264.7 Macrophage Cells", BioMed. Research International (2015)[DOI]
- Lawrence T, "Anti-inflammatory lipid mediators and insights into the resoution of inflammation", Nature Reviews Immunology, 2, pp.787~759 (2002)
- Nathan C., "Nitric oxide synthase: roles, tolls, and controls", Cell, 78, pp.915~918 (1984)
- MaCartney-Francis N., "Suppression of arthritis by an inhibitor of nitric oxide synthase", The Journal of Experimental Medicine, 178, pp.749~754 (1993)
- 김희정, "청도 반시의 과육과 감꼭지(시체: 柿滯)의 항산화 및 항염증 활성 연구", Journal of Applied Biological Chemistry, 54(2), pp.71~78 (2011), 한국응용생명화학회
- 강보경, "Lipopolysaccharide로 유도된 RAW 264.7 세포에서 다시마 뿌리에탄올 추출물의 항염증 효과", 한국식품과학회지, 46(6), pp.729~733 (2014), 한국식품과학회[DOI]
- 김보운, "LPS로 유도된 RAW 264.7 대식세포에 대한 애기외톨개 모자반(Myagropsis yendoi) 에틸아세테이트 분획물의 항염증 효과", 한국수산과학회지, 47(5), pp.527~536 (2014), 한국수산과학회
- 남상해, "두충 추출물이 DNCB로 유발된 알레르기성 접촉피부염과 산화적 손상에 미치는 영향", 한국식품영양과학회지, 36(12), pp.1517~1522 (2007), 한국식품영양과학회
- Chen W., "Role of p38 MAP kinases and ERK in mediating ultraviolet-B induced cyclooxygenase-2gene expression in human keratinocytes", Oncogene, 20, pp.3921~3926 (2001)
- Hanisch U. K., "Microglia as a source and target of cytokines", Glia, 40, pp.140~155 (2002)
- Beutler B., "The biology of cachectin", TNF-α primary mediator of the host response, 1989, pp.7, 625~655
- Balkwill F., "TNF-alpha in promotion and progression of cancer", Cancer and Metastasis Reviews, 25(3), pp.409~416 (2006)
- 배재천, "말초조직염증 혹은 신경손상 후 흰쥐 등쪽뿌리신경절에서 mitogen-activated protein kinase의 활성화", Anatomy and Cell Biology, 36(3), pp.9~246 (2003), 대한해부학회
- 양혜, "LPS 자극 RAW 264.7 대식세포에 있어서 아로니아 열매 열수 추출물의 항염증 효과", 한국식품영양과학회지, 44(1), pp.7~13 (2015), 한국식품영양과학회
- Li C. C., "Genipin inhibits lipopolysaccharideinduced acute systemic inflammation in mice as evidencedby nuclear factor-kappa B bioluminescent imaging-guided transcriptomic analysis", Food and Chemical Toxicology, 5, pp.2978~2986 (2012)
- Waetzig V., "c-Jun N-terminal kinases(JNKs)mediate pro-inflammatory actions of microglia", Glia, 50, pp.235~246 (2005)
- 주영현, "NC", Nga 마우스에서의 아토피피부염 모델 개발 및 피부염 병변에 대한 다래 추출물(Actinidia Extract)의 효과, 2009, pp.47(10), 1105~1112[DOI]
- 김영언, "송이즙의 항산화 활성, 혈전용해활성 및 Angiotensin Ⅰ Converting Enzyme의 저해활성 검색", 한국식품영양과학회지, 37(5), pp.535~541 (2008), 한국식품영양과학회
- 최원석, "둥근마를 이용한 기능성 건강음료개발에 관한 연구", 식품산업과 영양, 17(2), pp.20~22 (2012)
- 김미경, "블루베리 잎 추출물의 항산화 및 항염증 효과", 한국인체미용예술학회지, 15(4), pp.109~120 (2014), 한국인체미용예술학회
- 윤미연, "질경이 seed 추출물의 항산화 활성 및 미백작용에 관한 연구", 한국인체미용예술학회지, 14(2), pp.259~269 (2013), 한국인체미용예술학회
- 유선희, "백량금 열매 추출물이 항산화 및 항염작용에 미치는 영향", 한국인체미용예술학회지, 14(3), pp.197~208 (2013), 한국인체미용예술학회
- 김영례, "녹차 초임계 추출물의 피부 염증 유발균에 대한 항균활성 및 항산화 효과", 한국인체미용예술학회지, 15(2), pp.71~80 (2014), 한국인체미용예술학회