블루베리(Vaccinium ashei)잎 에탄올 추출물의 단회투여독성 및 피부 안전성 평가 연구
Oral Toxicity and Skin Safety Evaluation of Blueberry(Vaccinium ashei) Leaf Ethanol Extrac
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초록
This study investigated the single oral dose toxicity and skin safety, such as a primary skin irritation test, eye irritation test and skin sensitization test, of an ethanol extract of blueberry leaf(BBLE) to evaluate its use as a functional ingredient in cosmetics. In a single oral dose toxicity study, no differences were observed between the control and treated groups regarding clinical signs, weight gain, and gross findings. The results indicated that BBLE did not show any toxic effects at 10 mL/kg in mice, and the LD50 of BBLE was found to be higher than 10 mL/kg in this experiment. The results of a primary skin irritation test and eye irritation test in vitro showed that BBLE is included in the non-irritation area. Skin sensitization test results by Guinea Pig Maximization (GPMA) indicated that there was no allergic reaction. These results suggest that the ethanol extract of blueberry leaf in a 1% concentration can be a useful functional ingredient in cosmetics.
Abstract
This study investigated the single oral dose toxicity and skin safety, such as a primary skin irritation test, eye irritation test and skin sensitization test, of an ethanol extract of blueberry leaf(BBLE) to evaluate its use as a functional ingredient in cosmetics. In a single oral dose toxicity study, no differences were observed between the control and treated groups regarding clinical signs, weight gain, and gross findings. The results indicated that BBLE did not show any toxic effects at 10 mL/kg in mice, and the LD50 of BBLE was found to be higher than 10 mL/kg in this experiment. The results of a primary skin irritation test and eye irritation test in vitro showed that BBLE is included in the non-irritation area. Skin sensitization test results by Guinea Pig Maximization (GPMA) indicated that there was no allergic reaction. These results suggest that the ethanol extract of blueberry leaf in a 1% concentration can be a useful functional ingredient in cosmetics.
참고문헌 (22)
- 김계정, "화장품 발전 방향에 대한 피부과의 제안", 대한화장품과학회지, 24(2), pp.9~15 (1998)
- 이진, "화장품 소비욕구가 구매동기, 브랜드 만족, 애착 및 충성도에 미치는 영향", 한국의류학회지, 37(7), pp.882~893 (2013), 한국의류학회
- 장아람, "무궁화 (Hibiscus syriacus) 추출물의 화장품 생리 및 피부세포 활성 연구", 석사, 향장미용학과, pp.2012
- 김현희, "로즈마리 추출물의 화장품 생리활성 및 세포 독성 연구", 한국인체미용예술학회지, 14(4), pp.235~248 (2013), 한국인체미용예술학회
- 김영례, "동백나무 잎 추출물의 피부질환균에 대한 항균효과 및 항산화 활성", 대한미용학회지, 10(1), pp.13~19 (2014), 대한미용학회
- 이진만, "감나무(Diospyros kaki Thunb)잎 추출물에 대한 화장품약리활성 검증", 대한미용학회지, 9(4), pp.38~45 (2013), 대한미용학회
- 이진영, "도인승기탕 및 그 구성약재의 화장품약리활성", 대한본초학회지, 27(5), pp.65~75 (2012), 대한본초학회
- 안봉전, "한국산 약용식물의 화장품천연소재로서 응용에 관한 연구", 생명자원과 산업, 7, pp.58~77 (2003)
- 김용숙, "천연화장품 소재 탐색을 위한 명감나무잎 추출물과 분획물의 피부 균주에 대한 항균활성 조사", 한국미용학회지, 19(3), pp.557~564 (2013), 한국미용학회
- 최명신, "한국, 유럽 및 미국의 화장품 안전성 확보 동향", FDC 법제연구, 6(1), pp.19~28 (2011)
- Westwood, M. N., "Temperate-zone pomology", pp.100~101 (1993), Timber Press
- Azuma, K., "Phenolic antioxidant from the leaves of Corchorus olitorium I", J. Agric. Food. Chem, 47, pp.3963~3966 (1999)
- Ham, S. S., "Antimutagenic effects of juices from edible Korean wild herbs", J. Food Sci, Nutr, 2, pp.155~161 (1997)
- Martineau, L. C., "Anti-diabeti properties of the Canadian lowbush blueberry Vaccinium angustifolium Ait", Phytomedicine, 13, pp.612~623 (2006)
- Parry, J., "Chemical compositions, antioxidant capacities and antiproliferative activities of selected fruit seed flours", J. Agric. Food Chem, 54, pp.3773~3778 (2006)
- Seeram, N. P., "Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro", J. Agric. Food Chem, 54, pp.9329~9339 (2006)
- 이세나, "블루베리가 인체 유방암세포 MCF7에서 세포 사멸 관련 유전자 발현에 미치는 영향", 동아시아식생활학회지, 20(1), pp.30~36 (2010), 동아시아식생활학회
- 이준구, "배지의 종류와 조성이 Highbush 블루베리 삽수의 생육 및 발근에 미치는 영향", 원예과학기술지, 25(4), pp.355~359 (2007), 한국원예학회
- 식품의약품안전처, "의약품 등의 독성시험 기준. 식품의약품안전처 고시 제2009-116호", ~ (2009)
- 식품의약품안전처, "기능성화장품 등의 심사에 관한 규정. 식품의약품안전처 고시 제2009-166호", ~ (2009)
- Draize, J. H., "Appraisal of the safety of chemical in foods, drugs and cosmetics, the staff of the division of pharmacology" (1959), Food and Drug Education and Welfare, Pub
- Magnusson, B., "The identification of contact allergens by animal assay. The guinea pig maximization test", J. Inv. Derm, 52, pp.268~276 (1969)