경동맥 손상 및 혈전을 유발한 동물 모델에서 續命湯의 효능 검증
Effects of Sokmyong-tang on Ferric Chloride-induced Carotid Injury and Thrombosis in a Rat Model
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The aim of this study is examine the protection effect for artery and antithrombotic effect of Sokmyong-tang(SMT; xuming-tang) on carotid artery thrombosis in a rat model. Thirty minutes before FeCl3 treatment, SD rats were intraperitoneal injected with SMT. We tested the effects of SMT on time to occlusion (TTO) in thrombosis model by induced FeCl3 using the laser Doppler flow meter and accessed thrombus weight (TW) inhibition and measured collagen fibers in the vessel after injury using Masson's trichrome stain. SMT(100 mg/kg, i.p.) showed significantly delayed TTO (13.17±1.33 min, P < 0.001) compared to vehicle control group (8.63±0.92 min) and inhibiting effect on TW with 0.72±0.02 mg/mm (P < 0.05). In addition, SMT prevented collagen fibres damage in injured vessel (22.24±4.48%, P < 0.001). These results provide experimental evidence for SMT can be used to prevent vascular injury and thrombosis such as hypertension, arteriosclerosis, and so on.
Abstract
The aim of this study is examine the protection effect for artery and antithrombotic effect of Sokmyong-tang(SMT; xuming-tang) on carotid artery thrombosis in a rat model. Thirty minutes before FeCl3 treatment, SD rats were intraperitoneal injected with SMT. We tested the effects of SMT on time to occlusion (TTO) in thrombosis model by induced FeCl3 using the laser Doppler flow meter and accessed thrombus weight (TW) inhibition and measured collagen fibers in the vessel after injury using Masson's trichrome stain. SMT(100 mg/kg, i.p.) showed significantly delayed TTO (13.17±1.33 min, P < 0.001) compared to vehicle control group (8.63±0.92 min) and inhibiting effect on TW with 0.72±0.02 mg/mm (P < 0.05). In addition, SMT prevented collagen fibres damage in injured vessel (22.24±4.48%, P < 0.001). These results provide experimental evidence for SMT can be used to prevent vascular injury and thrombosis such as hypertension, arteriosclerosis, and so on.
참고문헌 (26)
- 양영이, "정향 추출물의 항산화·항혈소판 응집효과 및 혈전 용해능 탐색", 동의생리병리학회지, 25(3), pp.471~481 (2011), 대한동의병리학회
- Rader, D. J., "Translating molecular discoveries into new therapies for atherosclerosis", Nature, 451, pp.904~913 (2008)
- Lee, H, "How safe is the readministration of streptokinase?", Drug Saf, 13, pp.76~80 (1995)
- Burggraf, D., "Different therapy options protecting microvasculature after experimental cerebral ischaemia and reperfusion", Thromb Haemost, 103(5), pp.891~900 (2010)
- 백경민, "柿蒂의 in vitro와 in vivo 항혈전 효능 연구", 대한본초학회지, 26(4), pp.139~147 (2011), 대한본초학회
- Wang Ang., "YiFangJiJie", pp.153~154 (1992), DaXingWenHuaShe
- 李時珍, "本草鋼目", pp.571~572 (1993), 上海科學技術出版社
- 李克光, "金櫃要略譯釋", pp.137~138 (1993), 上海科學技術出版社
- Tohda, C., "Repair of amyloid β(25-35)-induced memory impairment and synaptic loss by a Kampo formula, Zokumei-to", Brian Res, 990, pp.141~147 (2003)
- Kurz, K. D., "Rat model of arterial thrombosis induced by ferric chloride", Thromb Res, 60, pp.269~280 (1990)
- Lee, I. S., "Optimization of ferric chloride induced carotid artery thrombosis model in a rat: Effect of Ginko biloba extracts.", Kor J Clinical Laboratory Sciences, 43(4), pp.179~187 (2011)
- Konstantinides, S., "Plasminogen activator inhibitor-1 and its cofactor vitronectin stabilize arterial thrombi after vascular injury in mice", Circulation, 103, pp.576~583 (2001)
- 양선아, "단삼 메탄올 추출물의 항혈전 및 항산화 효과", 한국식품과학회지, 39(1), pp.83~87 (2007), 한국식품과학회
- Deaths: Final Data for 2005, "Kung, H.", 56~ (2005)
- Ross R, "Atherosclerosis-an inflammatory disease", New Engl J Med, 340(2), pp.115~126 (1999)
- 이정희, "장기적인 인삼섭취가 인체의 혈전 관련 요인에 미치는 영향", 한국영양학회지, 28(9), pp.862~871 (2005)
- Mustard, J. F., "Factors influencing platelet function:adhesion, release and aggregation", Pharmacol Rev, 22, pp.97~137 (1970)
- Blaha, M. J., "A practical "ABCDE" approach Lo the metabolic syndrome", Mayo Clin.Proc, 83, pp.932~941 (2008)
- De Meyer SF, "Anitplatelet drugs", British J Hamatology, 142, pp.515~528 (2008)
- Liu, S., "Identification of inhibitors of the HIV-1 gp41 six-helix bundle formation from extracts of Chinese medicinal herbs. Prunella vulgaris and Rhizoma cibotte", Life Sci, 71, pp.1779~1791 (2002)
- 최승훈, "황제내경에서의 어혈의 인식에 대한 병리적 연구", 대전대학교논문집, 6(2), pp.313~320 (2006)
- Jeon, W. K., "Study on anti-thrombotic activities of KIOM2003-080", Korea J Ori Med, 13(2), pp.143~148 (2007)
- JongSooKim, "Inhibitory Effects of Silsosangami on the Platelet Aggregation", 동의생리병리학회지, 16(4), pp.30~829 (2002), 대한동의병리학회
- 이혜경, "加味通經湯의 항혈전, 소염 및 진통작용에 대한 연구", 대한한방부인과학회지, 16(1), pp.118~135 (2003), 대한한방부인과학회
- TaeWooPark, "A study on anti-thrombotic activity of Hwao-tang", 동의생리병리학회지, 16(1), pp.29~185 (2002), 대한동의병리학회
- 李德新, "氣血論", pp.161~ (1990), 遼寧科學技術出版社