염화 제 2철의 적합치가 유도하는 쥐의 경동맥 혈전증 모델 : 은행 나무 추출물의 효과
Optimization of Ferric Chloride Induced Carotid Artery Thrombosis Model in a Rat: Effect of Ginkgo biloba Extracts
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Abstract
Animal models are important tools in thrombosis research and preclinical drug development. In recent studies, ferric chloride (FeCl3) has been widely used to induce arterial thrombosis in a variety of species. The purpose of this study was to find an optimal concentration of FeCl3 and validate this model suited better for thrombosis research. A small piece of filter paper, soaked in FeCl3 solution (10, 20 or 35%, v/v, in distilled water) was topically applied on the carotid artery of SD rats to measure the time to occlusion (TTO) and thrombus weight (TW) to ascertain 35%, as an optimal FeCl3 concentration (8.63 ± 0.92 min; p =0.000, 0.79 ± 0.03 mg/mm; p =0.000, respectively). To validate this experimental model, Ginkgo biloba special extract EGb761 (5, 10 or 30 mg/kg) as a reference agent administered by peritoneal route for 1h prior to the induction of thrombosis, showed significantly delayed TTO in a dose dependent manner (18.50 ± 2.17, 29.17 ± 1.83, and 38.00 ±1.79 min, respectively) and significantly reduced TW and repaired collagen fibre in the injured vessel compare to vehicle group. Our results provide a simple, reproducible and well controlled in vivo screening system to induce thrombosis in rats by the topical application of 35% FeCl3 to assess the efficacy of the new anti-thrombotic agents.
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