蜈蚣 에테르 추출물의 RAW 264.7 cell에서 LPS로 유도된 염증반응 억제 효과
Inhibitory Effect of Scolopendra Morsitans L. Ether Extract on Lipopolysaccharide-Induced Inflammatory Response in RAW 264.7 Cells
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
It has been reported that Scolopendra morsitans L.(SML) has beneficial effects on human health and diverse diseases. The purpose of this study was to investigate the anti-inflammatory effects of ether extract from Scolopendra morsitans L. on lipopolysaccharide(LPS)-induced inflammatory response. Thus, we examined the inhibitory effect of SML ether fraction on LPS-induced increase of inflammatory mediators(NO, iNOS, COX-2, and IκBα) and pro-inflammatory cytokines(TNF-α) in RAW 264.7 cells. In the present study, SML ether extract itself decreased cell viability in a dose dependent manner(> 100 μg/ml). In addition, LPS increased NO production, iNOS expression and phosphorylation of Iκ-Bα, which were blocked by the treatment of SML ether fraction in a dose dependent manner. Furthermore, the treatment of LPS increased TNF-α production. However, the pretreatment of SML ether fraction prevented the LPS-induced TNF-α production in dose dependant manner. Taken together, our results suggest that SML may be a beneficial drug against inflammatory diseases such as sepsis.
Abstract
It has been reported that Scolopendra morsitans L.(SML) has beneficial effects on human health and diverse diseases. The purpose of this study was to investigate the anti-inflammatory effects of ether extract from Scolopendra morsitans L. on lipopolysaccharide(LPS)-induced inflammatory response. Thus, we examined the inhibitory effect of SML ether fraction on LPS-induced increase of inflammatory mediators(NO, iNOS, COX-2, and IκBα) and pro-inflammatory cytokines(TNF-α) in RAW 264.7 cells. In the present study, SML ether extract itself decreased cell viability in a dose dependent manner(> 100 μg/ml). In addition, LPS increased NO production, iNOS expression and phosphorylation of Iκ-Bα, which were blocked by the treatment of SML ether fraction in a dose dependent manner. Furthermore, the treatment of LPS increased TNF-α production. However, the pretreatment of SML ether fraction prevented the LPS-induced TNF-α production in dose dependant manner. Taken together, our results suggest that SML may be a beneficial drug against inflammatory diseases such as sepsis.
참고문헌 (22)
- Kim, S.N., "Study on genetic analysis and extract cytotoxicity of Scolopenda subspinipes mulitilans", 석사, pp.2005
- 중약대사전편찬위원회, "중약대사전", pp.3026~3030 (1997), 도서출판 정담
- 이동희, "오공의 항고혈압작용에 관한 연구", 대한본초학회지, 12(2), pp.39~49 (1997)
- 정병태, "오공수침이 진통 및 진경효과에 미치는 영향", 대한침구학회지, 14(2), pp.219~230 (1997)
- 최용건, "蜈蚣의 항산화효과에 관한 연구", 동의신경정신과학회지, 19(3), pp.129~142 (2008), 대한한방신경정신과학회
- 김성철, "水醇추출법으로 조제된 오공 약침액의 생리활성 효과", Journal of Pharmacopuncture, 13(3), pp.5~14 (2010), 대한약침학회
- 조일주, "마우스 대식세포주인 RAW 264.7 세포에서 蜈蚣의 항염증 효과", 대한본초학회지, 26(3), pp.23~29 (2011), 대한본초학회
- Kim, G. S., "Effects of Scolopendrae corpus on immune response in mice of different ages", Korean J. Orient Int Med, 19, pp.477~490 (1998)
- 송인석, "경희약대논문집", 경희약대논문집, 24, pp.91~93 (1996)
- 홍남두, "오공의 약물학적 연구", 경희약대논문집, 5, pp.20~27 (1977)
- 생약학교재편찬위원회, "생약학", pp.679~680 (2008), 동명사
- Erwig, L. P., "Macrophage activation and programming and its role for macrophage function in glomerular inflammation", Kidney Blood Press Res, 22(1), pp.21~25 (1999)
- McDaniel, M. L., "Cytokines and nitric oxide in islet inflammation and diabetes", Proc Soc Exp Biol Med, 211, pp.24~32 (1996)
- Willeaume, V., "Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages : similarities and differences", J Inflamm, 46, pp.1~12 (1996)
- Takeda, K., "Toll-like receptors", Annu Rev Immunol, 21, pp.335~376 (2003)
- Lu, C. N., "Saikosaponin a and its epimer saikosaponin d exhibit anti-inflammatory activity by suppressing activation of NF-κB signaling pathway", Int Immunopharmacol, 14(1), pp.121~126 (2012)
- 김대희, "LPS로 활성화된 대식세포에서 黃連解毒湯 물추출물의 염증매개물질 억제효과", 대한본초학회지, 24(4), pp.39~47 (2009), 대한본초학회
- 박상미, "桑葉 추출물의 LPS로 유도된 RAW 264.7 세포에서의 항염증 효과", 대한본초학회지, 27(3), pp.31~38 (2012), 대한본초학회
- La, Sala, A., "G(i)-protein-dependent inhibition of IL-12 production is mediated by activation of the phosphatidylinositol 3-kinase-protein 3 kinase B", Akt pathway and JNK, 2005, pp.175, 2994~2999
- Wang, M. T., "Cyclooxygenases, prostanoids, and tumor progression", Cancer Metastasis Rew, 26, pp.525~534 (2007)
- Delgado, A. V., "Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides, 37, pp.355~361 (2007)
- Kranzhofer R, Schmidt J, Pfeiffer C. A., "Angiotensin induces inflammatory activation of human vascular smooth muscle cells", Arterioscler Thromb Vasc Biol, 19, pp.1623~1629 (1999)