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Papain으로 유도된 골관절염 생쥐 모델에서 四逆湯의 항골관절염 효능에 관한 연구

Effects of Sayeok-tang on Papain-Induced Osteoarthritis in Mice

오민석|공상은
한의병리학회 (The Society of Pathology in Korean Medicine)|2013.04|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제27권 제2호|pp. 212-224

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참고문헌

키워드

OsteoarthritisCytokinemRNAArachidonic acidSayeok-tang(SYT)Papain-induced osteoarthritis
주제분류한의학

초록

This study intends to clarify how Sayeok-tang(here in after reffered to SYT) affect C57BL/10 mice whose osteoarthritis was induced by papain. Osteoarthritis was induced by injecting papain in the knee joint of 3 groups(n=6) of mice. Normal group was non-treatment group and was not injected papain, whereas control mice were orally administered with 200 ㎕ of physiological saline. Positive comparison group was medicated with 100 mg/kg of Joins® mixed with 200 ㎕ of physiological saline. Experimental group was medicated with 400 mg/kg of SYT mixed with 200 ㎕ of physiological saline. Both Positive and experimental comparison groups were orally medicated once per day for 4 weeks. After the experiment, the functions of liver and kidney, inflammation cytokine values within serum, degree of revelation for inflammation cytokine genes, immune cells within blood, metabolism of arachidonic acid and amount of cartilage were measured and histopathological changes in the knee joint structures were observed. As results, SYT had no significant effect on the liver and kidney functions. Interleukin-1β(IL-1β), interleukin-6(IL-6), monocyte chemo attractant protein-1(MCP-1) and tumor necrosis factor-α(TNF-α) were significantly decreased. Inflammation cytokines in joints were all significantly decreased. Prostaglandin E2(PGE2), thromboxane B2(TXB2) were significantly decreased. Destruction of cartilage on micro computed tomography(CT)-arthrography was meaningfully decreased. In terms of histopathology, infiltration of inflammation, proliferation of synovial membrane, subsidence of cartilage and bone due to penetration of excessive formation of synovial cell and destruction of cartilage were small. Based on all results mentioned above, Sayeok-tang(SYT) is believed to be meaningful for suppressing the progress of osteoarthritis and its treatments because of its anti-inflammatory effects and alleviation of pain with histopathological effective efficacy.

Abstract

This study intends to clarify how Sayeok-tang(here in after reffered to SYT) affect C57BL/10 mice whose osteoarthritis was induced by papain. Osteoarthritis was induced by injecting papain in the knee joint of 3 groups(n=6) of mice. Normal group was non-treatment group and was not injected papain, whereas control mice were orally administered with 200 ㎕ of physiological saline. Positive comparison group was medicated with 100 mg/kg of Joins® mixed with 200 ㎕ of physiological saline. Experimental group was medicated with 400 mg/kg of SYT mixed with 200 ㎕ of physiological saline. Both Positive and experimental comparison groups were orally medicated once per day for 4 weeks. After the experiment, the functions of liver and kidney, inflammation cytokine values within serum, degree of revelation for inflammation cytokine genes, immune cells within blood, metabolism of arachidonic acid and amount of cartilage were measured and histopathological changes in the knee joint structures were observed. As results, SYT had no significant effect on the liver and kidney functions. Interleukin-1β(IL-1β), interleukin-6(IL-6), monocyte chemo attractant protein-1(MCP-1) and tumor necrosis factor-α(TNF-α) were significantly decreased. Inflammation cytokines in joints were all significantly decreased. Prostaglandin E2(PGE2), thromboxane B2(TXB2) were significantly decreased. Destruction of cartilage on micro computed tomography(CT)-arthrography was meaningfully decreased. In terms of histopathology, infiltration of inflammation, proliferation of synovial membrane, subsidence of cartilage and bone due to penetration of excessive formation of synovial cell and destruction of cartilage were small. Based on all results mentioned above, Sayeok-tang(SYT) is believed to be meaningful for suppressing the progress of osteoarthritis and its treatments because of its anti-inflammatory effects and alleviation of pain with histopathological effective efficacy.

참고문헌 (51)

  1. 한방재활의학과학회, "한방재활의학과학", pp.84~98 (2011), 군자출판사
  2. Patrick, D. W., "Textbook of pain", pp.575~589 (2002), 정담
  3. "장래인구추계(2010～2060)", 통계청[URL]
  4. 정영복, "정형외과학 제 6 판", pp.253~258 (2006), 최신의학사
  5. 송영준, "골관절염의 치료에서 COX-2 선택적 비스테로이드성 소염진통제의 이용", 대한슬관절학회지, 21(2), pp.84~92 (2009)
  6. 골관절염 환자에서 글루코사민의 임상적 효과, "김가은", 한국보건의료연구원, 1~83, pp.Neca-Rapid 보고서 11-002 (2011)
  7. 서승석, "슬관절의 대퇴골과 골연골 결손에 대한 자가 골연골 이식술", 대한정형외과학회지, 44(3), pp.301~310 (2009), 대한정형외과학회
  8. Kuo, A. C., "Microfracture and bone morphogenetic protein 7(BMP-7) synergistically stimulate articular cartilage repair", Osteoarthritis Cartilage, 14, pp.1126~1135 (2006)
  9. 김덕희, "活絡丹의 Adjuvant 유발 관절염 진행 억제 효과", 박사, 한의학, pp.2009
  10. 임동욱, "한약복합물 HT008이 흰쥐 관절염 모델에 미치는 효과", 대한본초학회지, 24(4), pp.197~204 (2009), 대한본초학회
  11. 박동수, "蠲痺湯이 Monosodium Iodoacetate로 유발된 골관절염의 초기변화에 미치는 영향", 동의생리병리학회지, 25(1), pp.84~91 (2011), 대한동의병리학회
  12. 김지영, "부자탕이 Monosodium Iodoacetate로 유발된 골관절염의 초기변화에 미치는 영향", 한방재활의학과학회지, 21(2), pp.87~100 (2011), 한방재활의학과학회
  13. 이승헌, "秦艽⋅威靈仙⋅夏枯草 복합방이 Monosodium Iodoacetate로 유발된 흰쥐의 골관절염에 미치는 영향", 한방재활의학과학회지, 18(1), pp.75~94 (2008), 한방재활의학과학회
  14. 노의준, "古方類聚", pp.592~596 (2009), 고방출판사
  15. 복치의학회, "도해유취방", pp.324~325 (2010), 복치의학회출판국
  16. 李載熙, "도설 한방진료요방", pp.395~397 (2004), 의방출판사
  17. 한태륜, "재활의학", pp.905~908 (2009), 군자출판사
  18. 진수희, "2009년 국민건강통계. 국민건강영양조사 제4기 3차년도(2009)", pp.2010~ (2009), 보건복지부 건강정책과
  19. 김현아, "퇴행성 관절염의 임상연구", 대한류마티스학회지, 7(3), pp.205~211 (2000)
  20. 김기현, "痺證", pp.23~29 (1993), 정담출판사
  21. 王琦, "黃帝內經素問今釋", pp.206~ (1983), 成輔社
  22. 蔡仁植, "傷寒論譯詮", pp.9~141 (1984), 高文社
  23. 張仲景, "金匱要略方論", pp.30~39 (1950), 大聯國風出版社
  24. "Herbal healing" (2002)[URL]
  25. 황도연, "대역 증맥 방약합편", pp.129~ (1992), 남산당
  26. 정선희, "부자약침이 진통 및 소염작용에 미치는 영향", 대한침구학회지, 14(1), pp.334~336 (1997)
  27. 김윤희, "부자약침이 흰쥐의 Collagen 유발 관절염에 미치는 영향", 박사, 침구학, pp.2006
  28. 朱自平, "부자온중지통약리연구", 중국중약잡지, 17(4), pp.238~241 (1992)
  29. 육창수, "한약의 약리, 성분, 임상응용", pp.695~699 (1982), 계축문화사
  30. 송인성, "감초건강탕의 임상적 효능에 관한 소고", 석사, 임상약학, pp.2000
  31. Nergis, M., "Quantification of papain-induced rat osteoarthritis in relation to time with the mankin score", Acta orthop traumatol turc, 41(3), pp.233~237 (2007)
  32. George, B., "Papain-induced degenerative arthritis of the hip in rabbits", The journal of bone and joint surgery, 53(2), pp.324~337 (1971)
  33. Panickery, S., "Oral glucosamine modulates the response of the liver and lymphocytes of the mesenteric lymph nodes in a papain-induced model of joint damage and repair", Osteoarthritis and Cartilage, 17, pp.1014~1021 (2009)
  34. 이삼규, "단일관절염 유발 백서에서의 관절통의 동적 정량화", 박사, 의과, pp.2002
  35. Dinarello, C.A., "The interleukin-1 family: 10years of discovery", FASEB J, 8(15), pp.1314~1325 (1994)
  36. Hulejova, H., "Increased level of cytokines and matrix metalloproteinases in osteoarthritic subchondral bone", Cytokine, 38(3), pp.151~156 (2007)
  37. Martel-pelletier, J., "Cyclooxygenase-2 and prostaglandins in articular tissues", Semin Arthritis Rheum, 33(3), pp.155~167 (2003)
  38. Simi, T., "Inhibition of IL-6, IL-10 Signaling and star activation by inflammatory and stress pathways", J. Immunol, 165, pp.5227~5237 (2000)
  39. Jacqueline, U., "Soluble IL-6 receptor and different arthropathies", J. Rheumatol, 24, pp.2069~2075 (1997)
  40. Katherine, L., "Cytokine level in synovial fluid and serum of patient with juvennile rheumatoid arthritis", J. Rheumatol, 21, pp.2359~2363 (1994)
  41. Conti, P., "Monocyte chemotactic protein-1 provokes mast cell aggregation and〔3H〕5HT release", Immunology, 86(3), pp.434~440 (1995)
  42. Bischoff, S. C., "Monocyte chemotactic protein 1 is a potent activator of human basophils", J. Exp. Med, 175(5), pp.1271~1275 (1992)
  43. 백대현, "흰쥐에서 Leukotriene B4와 Thromboxane B2가 요추 신경근에 미치는 영향", 대한척추외과학회지, 8(1), pp.8~14 (2001)
  44. Ford-hutchinson, A. W., "a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes", Natur, 286, pp.264~265 (1980)
  45. Roitt, I., "Immunology", pp.66~69 (1998), Mosby
  46. Kasper, "Harrison's 내과학", pp.2083~2084 (2006), 도서출판
  47. Hirai, Y., "Effects of nitric oxide on matrix metalloproteinase-2 production by rheumatoid synovial cells", Life Sci, 68(8), pp.913~920 (2001)
  48. Beuerova, K., "Role of reactive oxygen and nitrogen species in etiopathogenesis of rheumatoid arthritis", Gen Physiol Biophys, 18, pp.15~20 (1999)
  49. Aley, K. O., "Nitric Oxide signaling in pain and nociceptor sensitization in the rat", J Neurosci, 18(17), pp.7008~7014 (1998)
  50. Smith, W. L., "Prostaglandin endoperoxide H synthase (cyclooxygenase)-1 and 2", Adv immunol, 62, pp.167~215 (1996)
  51. Brune, K., "Selective cyclooxygenase-2 inhibitors: similarities and diffences", Scand J Pheumatol, 33, pp.1~6 (2004)

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