OP9 세포에서 포도당 흡수능에 대한 대계 추출물의 효과
Effects of Herba Cirsii Extracts on Glucose Uptake in OP9 Cells
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Although the Herba Cirsii is known to posses beneficial health effects, the anti-diabetic effects and the mechanism of action have not been elucidated. In the present study we have shown that Herba Cirsii Extract (HCE) can stimulate glucose uptake in OP9 adipocytes. Unlike insulin, HCE did not stimulate the Ser473 phosphorylation and activation of Akt. The increasing effects of HCE on glucose uptake were inhibited by PD680509 and compound C pretreatment, which means that the glucose uptake effects by HCE were carried out by extracelluar signal-regulated kinase1/2(ERK1/2) and AMP-activated protein kinase (AMPK) activation. Further studies revealed that HCE stimulated glucose transport occurs through a mechanism involving ERK1/2 activation and AMPK activation.
Abstract
Although the Herba Cirsii is known to posses beneficial health effects, the anti-diabetic effects and the mechanism of action have not been elucidated. In the present study we have shown that Herba Cirsii Extract (HCE) can stimulate glucose uptake in OP9 adipocytes. Unlike insulin, HCE did not stimulate the Ser473 phosphorylation and activation of Akt. The increasing effects of HCE on glucose uptake were inhibited by PD680509 and compound C pretreatment, which means that the glucose uptake effects by HCE were carried out by extracelluar signal-regulated kinase1/2(ERK1/2) and AMP-activated protein kinase (AMPK) activation. Further studies revealed that HCE stimulated glucose transport occurs through a mechanism involving ERK1/2 activation and AMPK activation.
참고문헌 (17)
- 신민교, "임상본초학", pp.495~496 (1986), 남산당
- 박성규, "DMN으로 유발된 흰쥐의 간섬유화에 미치는 대계의 효과", 동의생리병리학회지, 18(2), pp.413~418 (2004), 대한동의병리학회
- 이정주, "Antioxidant Property of Aqua-Acupuncture Solution from Circium japonicum", Korean Journal of Acupuncture, 22(4), pp.57~65 (2005), 경락경혈학회
- 이정주, "肝兪？中脘의 大？ 藥鍼이 급성 산화적 간손상에 미치는 효과", Korean Journal of Acupuncture, 20(4), pp.4~52 (2003), 경락경혈학회
- Krentz, A.J., "Insulin resistance", BMJ, 313, pp.1385~1389 (1996)
- Cefalu, W.T., "Insulin resistance: celluar and clinical concepts", Exp Biol Med, 226, pp.13~26 (2001)
- James, D.E., "Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein", Nature, 333, pp.183~185 (1998)
- Cushman, S.W., "Potential mechanism of insulin action on glucose transport in the isolated rat adipose cell. Apparent translocation of intracellular transport systems to the plasma membrane", J Biol Chem, 255, pp.4758~4762 (1980)
- Marette, A., "Abundance, localization, and insulin-induced translocation of glucose transporters in red and white muscle", Am J Physiol, 263, pp.C443~C452 (1992)
- Fujii, N., "AMP-activated protein kinase and the regulation of glucose transport", Am J Physiol Endocrinol Metab, 291, pp.E867~E877 (2006)
- Wolins, N.E,., "OP9 mouse stromal cells rapidly differentiate into adipocytes: characterization of a useful new model of adipogenesis", Journal of Lipid Research, 47, pp.450~460 (2006)
- Abdin, A.A., "Effects of rosiglitazone and aspirin on experimental model of induced type 2 diabetes in rats: focus on insulin resistance and inflammatory markers", J Diabetes Complications, 24, pp.168~178 (2010)
- Hayden, M.R.., "Treating hypertension while protecting the vulnerable islet in the cardiometabolic syndrome", J Am Soc Hypertens, 2, pp.239~266 (2008)
- Fukushima, T., "Insulin receptor substrates form high-molecular-mass complexes that modulate their availability to insulin", insulin-like growth factor-I receptor tyrosine kinases, 2011, pp.404, 767~773
- Zhu, Y., "Cardiac PI3K-Akt impairs insulin-stimulated glucose uptake independent of mTORC1 and GLUT4 translocation", Mol Endocrinol, 27, pp.172~184 (2013)
- Kramer, H.F., "Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle", Diabetes, 55, pp.2067~2076 (2006)
- Chen, H.C., "Activation of the ERK pathway and atypical protein kinase C isoforms in exercise- and aminoimidazole-4-carboxamide-1-beta-D-riboside (AICAR)-stimulated glucose transport", J Biol Chem, 277, pp.23554~23562 (2002)