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반지련으로부터 분리한 luteolin의 세포고사효과

Apoptotic Effect of Luteolin Isolated from Scutellaria barbata

박영두|김성훈|안규석|김진형|이은옥
한의병리학회 (The Society of Pathology in Korean Medicine)|2005.08|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제19권 제4호|pp. 955-959

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주제분류한의학

초록

Abstract

We previously demonstrated that the methylene chloride fraction of Scutellaria barbata suppessed human leukemic U937 cell proliferation by inducing apoptosis. In the present study, we have isolated luteolin from Scutellaria barbata and evaluated its apoptotic mechanism in Lewis lung carcinoma cells. Luteolin inhibited the proliferation of Lewis lung carcinoma cells in a concentration-dependent manner. Luteolin effectively increased the portion of sub-G1 DNA content (apoptotic portion) and apoptotic Annexin-V positive cells in a concentration-dependent manner by FACS analysis. Caspase 9 and caspase 3 were activated and PARP was effectively cleaved by luteolin. It also increased the ratio of Bax to Bcl-2 through the decrease of Bcl-2 expression by Western blotting and reduced mitochondrial membrane potential following TMRE staining. These results suggest that luteolin can induce apoptosis through the mitochondrial mediated pathway.

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관련 논문AI추천

과루인 Methylene Chloride층의 세포고사 유도 효과강인철, 김성훈, 박영두, 안규석, 이은옥, 이주령, 차윤이한의병리학회 · 동의생리병리학회지2003 · 제17권 제3호반지련의 항암활성에 관한 연구김동일, 윤종원, 이태균대한한방부인과학회 · 대한한방부인과학회지2005 · 제18권 제2호半枝蓮이 HeLa Cell의 增殖抑制와 死滅에 미치는 影響이경섭, 장준복, 조정훈, 하지연대한한방부인과학회 · 대한한방부인과학회지2006 · 제19권 제4호황금으로부터 항산화 활성 성분의 분리김석창한국약용작물학회 · 한국약용작물학회지2006 · 제14권 제4호하고초 ethyl acetate분획의 U937세포에 대한 세포고사 유도효과김성훈, 이은옥한의병리학회 · 동의생리병리학회지2003 · 제17권 제2호Bufalin이 U937세포의 Apoptosis에 미치는 영향박민철, 육상원, 이광규, 이상룡, 이창현, 정한솔한의병리학회 · 동의생리병리학회지2001 · 제15권 제3호

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