봉독약침이 연골세포 활성에 미치는 영향
Effect of Bee Venom Acupuncture on the Recovery of Chondrocyte Phenotype in Rabbit Cartilage
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
Articular cartilage is an important factor for studying the arthritic diseases. The chondrocyte isolated from cartilage has the characteristic of differentiation and re-differentiation when cultured in monolayer. The bee venom (BV) acupuncture was investigated to examine their abilities on chondrocyte re-differentiation via rabbit chondrocyte primary culture. And the expression profiles of type II collagen (COL2) was analyzed using RT-PCR and western hybridization at third passage chondrocyte. In general, the mRNA expression of type II collagen was reduced step by step with the subculture of the chondrocyte. However, after the BV treatment for 48hr at third passage, the expressions of type II collagen were found to be significantly up-regulated, the same result was confirmed by western blotting. These results suggested that the diluted solution of BV for herb-acupuncture was very effective on the recovery of articular chondrocyte phenotype.
참고문헌 (28)
- 서동민, "蜂毒藥鍼이 adjuvant 誘發 關節炎에 미치는 鎭痛效果 및 그 機轉에 관한 硏究", 20(2), pp.85~97 (2003)
- Yoon, "Maintenance of differentiated phenotype of articular chondrocytes by protein kinase C and extracellular signal-regulated protein kinase The Journal of Biological chemistry 277", 277(10), pp.8412~8420 (2002)
- Mika Tadokoro, "Chondrogenic differentiation of human mesenchymal stem cells cultured in a cobweb-like biodegradable scaffold", 322, pp.50~55 (2004)
- Watt,F, "Effect of seeding density on stability of the differentiated phenotype of pig articular chondrocytes in culture", 89, pp.373~378 (1988)
- Benya, "De-differentiated chondrocytes re-express the differentiated collagen phenotype when cultured in agarose gels", pp.373~384 (1982)
- Ng, "Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads", pp.97~104 (1994)
- Molly, "Journal of Orthopaedic Research 22", 22, pp.1114~1119 (2004)
- Weisser, "Role of growth factors in rabbit articular cartilage repair by chondrocytes in agarose", 9(Supplement A), pp.48~54 (2001)
- 高炯均, "봉독침요법이 항염, 진통 및 해열에 미치는 효능에 관한 실험의 연구", 13(1), pp.283~292 (1992)
- 都垣錫, "봉독요법이 횐쥐의 슬관절 염증성 부종에 미치는 영향", 12(1), pp.211~220 (1995)
- 孔賢淑, "봉독침요법이 항경란에 미치는 영향", 10(1), pp.159~165 (1993)
- 李宗錫, "약침용 봉독액의 급성독성에 관한 연구", 11(1), pp.177~195 (1994)
- 李泓錫,, "봉독약침자극이 Methothrxate로 유발된 생쥐의 면역기능저하에 미치는 영향", 21(1), pp.347~359 (1998)
- Barbara Rudolf, "Chemistry and pharmacology of honey bee venom", pp.329~402 (1986)
- Spoerri, P, "Apamin from bee venom", pp.207~3 214 (1973)
- 서병관, "退行性關節炎 韓方治療에 對한 最近 硏究 動向 -臨床硏究 方法論을 中心으로", 21(3), pp.265~282 (2004)
- Friedman, "Preliminary results with abrasion arthroplasty in osteoarthritic knee"
- 성진형, "홍삼사포닌이 사람의 관절연골세포에 미치는 영향", 33(7), pp.1921~1927 (1998)
- 김범수, "토끼의 성장판 연골세포에서 배양방법에 따른 TGF-β1의 역할 변화", 34(5), pp.849~857 (1999)
- 이영재, "퇴행성 슬관절염에 대한 침치료 및 연구의 임상고찰", 11(1), pp.465~472 (1994)
- 이성노, "蜂藥鍼治療의 退行性膝關節炎에 대한 臨床의 考察", 20(5), pp.73~81 (2003)
- 왕오호, "퇴행성 슬관절염의 봉독약침 치료효과에 대한 임상의 관찰", 18(3), pp.35~47 (2001)
- Kim, M.J.,, "Effects of deer antler Water Extract (pilose antler of Cervus Korean TEMMINCK var. mantchuricus Sinhoe) on chondrocytes", 21(2), pp.73~88 (2004)
- 이상훈, "봉독약침이 류마티스 관절염 환자의 관절 통증, 종창 및 급성 염증 반응에 미치는 영향", 20(2), pp.77~84 (2003)
- David, "Regulation of type-II collagen gene expression during human chondrocyte de-differentiation and recovery of chondrocyte-specific phenotype in culture involves Sry-type high-mobility-group box", 360, pp.461~470 (2001)
- Laurence Borge, "Type II transglutaminase expression in rabbit articular chondrocytes in culture relation with cell differentiation", 1312, pp.117~124 (1996)
- Jakob, "Chondrogenesis of expanded adult human articular chondrocyte is enhanced by specific prostaglandins", 43, pp.852~857 (2004)
- 朴贊烈,, "약침용봉독액이 흑색종세포에 미치는 항암효과에 대한 분자생물학적 연구", 2000, pp.183~200 (2001)