마이크로어레이를 이용한 봉독 처리된 돼지 초대배양 연골세포에서의 유전자 발현 프로파일링
Gene Expression Profiling in Porcine Primary Chondrocyte Cells Treated with Bee Venom using Microarray
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초록
골관절염(osteoarthritis)은 연령이 증가하면서 발병율이증가하고 있으며 성인에서 흔하게 발생하는 중요한 질환이다. 이러한 골관절염의 치료는 외과적 치료방법과 소염진통제류(non-steroidal anti-inflammatory drugs, NSAIDs)의약물치료가 있지만 부작용이 증가하는 등, 치료수단으로는미흡한 점이 많다. 한편 봉독은 대표적인 천연물질로 각종염증, 통증성 질환에 활용되어 관절염에 미치는 효과에 대한 연구가 많이 진행되어 왔으나 봉독이 연골세포 분화에
Abstract
Osteoarthritis is one of the commonest causes associated with age-related damage of articular cartilage. Non-steroidal anti-inflammatory drugs are commonly used in osteoarthritic patient. However, long term administration of these drugs results gastrointestinal disorders. Though, most studies have demonstrated in the past that bee venom has therapeutic effect on diseases related to inflammation and pains, but its anti-inflammatory properties have not been so far studied on inflamed chondrocytes (LPS induced) invitro. For the purpose, the study was carried out to determine the effect of bee venom on porcine articular chondrocyte cell using microarray. In this study, we found that 2,235 significantly associated gene (1,404 up-regulated genes and 831 down-regulated genes) that were expressed on inflamed and non inflamed chondrocytes during proliferation. Among the 1,404 up-regulated genes and 831 down-regulated genes, known genes were 372 and 237, respectively. On the other hand, bee venom significantly reduced expression of fetuin involved in acute inflammatory reaction. Our results suggest that this study could be useful database in gene expression profiling of chondrocyte cell treated with bee venom.
참고문헌 (11)
- Altman, R., "Development of criteria for the classification and reporting of osteoarthritis: classification of osteoarthritis of the knee", Arthritis Rheum, 29(8), pp.1039~1049 (1986)
- 안현진, "봉독이 Papain으로 유도된 골관절염 동물 모델에 미치는 영향", 생약학회지, 43(2), pp.167~172 (2012), 한국생약학회[DOI]
- 방준석, "골관절염 치료제 히알우론산 임상연구결과의 Systemic Review", 한국임상약학회지, 17(1), pp.38~45 (2007), 한국임상약학회
- Dunn, J. D., "Effect of melittin on pituitary-adrenal responsiveness to stress", Acta. Endocrinol, 119(3), pp.339~344 (1998)
- Gi, L. K., "An experimental study with bee venom therapy on anti-inflammatory and analgesic effects", The Journal of Korean Acupuncture & Moxibustion Society, 15(2), pp.97~103 (1998)
- 김선광, "Effects of bee venom on the pro-inflammatory responses in RAW264.7 macrophage cell line", JOURNAL OF ETHNOPHARMACOLOGY, 99(1), pp.157~160, ELSEVIER IRELAND LTD
- Lee, S. S., "Clinical efficacy safety of Hyuanⓡ (sodium Hyaluronate) in patients with osteoarthritis of the knee", Journal of Rheumatic Diseases, 6(1), pp.53~61 (1996)
- 이상훈, "봉독약침이 류마티스 관절염 환자의 관절 통증, 종창 및 급성 염증 반응에 미치는 영향", 대한침구의학회지, 20(2), pp.7~ (2003), 대한침구의학회
- King-Woo Nam, "Inhibition of COX-2 Activity and Proinflammatory Cytokines (TNF-α and IL 1 β) Production by Water-Soluble Sub-Fractionated Parts from Bee(Apis mellifera) Venom", Archives of Pharmacal Research, 26(5), pp.383~388 (2003), 대한약학회
- 노성숙, "퇴행성 슬관절염에 대한 히알루론산 나트륨의 치료효과", The Korean Journal of Pain, 17(2), pp.12~12 (2004), 대한통증학회
- Wang, W. H., "clincal investigation compared with the effectsof the bee venom-acupuncture on knee joint withosteoarthritis", The Journal of Korean Acupuncture &Moxibustion Society, 18(3), pp.35~47 (2001)