加味腎氣湯 및 加味淸肺湯이 기도점액 분비 및 기관평활근 긴장도에 미치는 영향
Effects of Gamisingi-tang and Gamicheongpye-tang on Airway Mucus Secretion
1
조회
0
다운로드
5
인용
0
참고문헌
초록
Abstract
In the present study, the author intended to investigate whether two oriental medical prescriptions named GSGT and GCPT significantly affect mucin release from cultured hamster tracheal surface epithelial (HTSE) cells. Confluent HTSE cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presen+ce of GSGT or GCPT to assess the effect of each agent on 3H-mucin release. Possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase(LDH) release. Also, the effects of GSGT and GCPT on contractility of isolated tracheal smooth muscle were investigated. (1) GSGT did not affect mucin release without cytotoxicity ; (2) GCPT significantly stimulated mucin release from cultured HTSE cells, with significant cytotoxicity ; (3) GSGT and GCPT did not affect contractility of isolated tracheal smooth muscle. We suggest that the effects of GCPT and its components should be further investigated and it is of great value to find, from oriental medical prescriptions, novel agents which have potent expectorant effects on mucin secretion from airway goblet cells.
참고문헌 (17)
- "소아만성 재발성 호흡기 증상에 대한 고찰", 16(2), pp.84~ (2002)
- "설치류 기관 뮤신유리 억제에서의 폴리양이온성 작용기전" (1997), 서울대학교대학원
- "textbook of pulmonary disease" (1983), Little Brown and Company
- "Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma", pp.345~353 (1981)
- "Drug actions", pp.410~411 (1995), CRC press Inc
- "Secretions from primary hamster tracheal surface epithelial cells in culture", pp.299~314 (1989), Opaskar-Hincman
- "Possible requirement of collagen gel substratum for production of mucinlike glycoproteins by primary rabbit tracheal epithelial cells in culture", pp.617~621 (1985)
- "Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture", pp.4021~4027 (1985)
- "Gel contraction causes mucin release in primary hamster tracheal epithelial cells growing on a collagen gel", pp.105~ (1987)
- "Continuous multiplication of rabbit tracheal epithelial cells in a defined", pp.800~881 (1982)
- "Expression of tracheal differentiated function in serum-free hormone- supplemented medium", pp.167~181 (1985)
- "東醫小兒科學", pp.28~30 (2002), 정담출판사
- "동의폐계내과학", pp.52 102 144~55 114 199 (2002), 한문화사
- "Involvement of a signal transduction mechanism in ATP-induced mucin release from cultured airway goblet cells", pp.121~125 (1993)
- "Measurement of viability and cytotoxicity Culture of animal cells" (1994), Inc
- "Physiologic modulation of bronchial epithelial cell barrier function by polycationic exposure" (1994)
- "Physiologic modulation of bronchial epithelial cell barrier function by polycationic exposure" (1994)