흑삼의 제조 및 항암효과
Preparation of Black Ginseng and its Antitumor Activity
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Abstract
The purpose of this study is to prepare black ginseng and evaluate its antitumor activity. In order to achieve such aim, 5 year fresh ginsenges were steamed at 95℃ for 3 hr in pottery apparatus and dried at 60℃ for 12-36 hr. This process was repeated again nine times in same condition. Among the ginseng saponins in black ginseng, the amount of Ginsenoside Rg3 was examined by HPLC. 10.05 ㎎ of Ginsenoside Rg3 was obtained from 1 g of dried black ginseng prepared. The extract of black ginseng exhibited stronger cytotoxic activity against MCF-1, HT-1080 and Hepa 1C1C7 tumor cell lines in vitro than the extract of red ginseng. Also, the extract of black ginseng exhibited stronger antitumor activity(33%) in BDF1 mice bearing Lewis lung carcinoma cells(LLC) than the extract of red ginseng(23%). From these results, it was concluded that black ginseng had antitumor activity suggesting its application for the prevention and treatment of cancer.
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