파삼의 항암활성 증진이 가능한 고압 추출 공정
High Pressure Extraction Process of Low Quality Fresh Ginseng for Enhancing Anticancer Activities
2
조회
0
다운로드
2
인용
0
참고문헌
초록
Abstract
The low quality fresh ginseng was extracted by water at 80℃ and 240 bar for 20 min (HPE, High pressure extraction process). The cytotoxicity on human normal kidney cell (HEK293) and human normal lung cell (HEL299) of the extracts from HPE showed 28.43% and 21.78% lower than that from conventional water extraction at 100℃ in adding the maximum concentration of 1.0 ㎎/㎖. The human breast carcinoma cell and lung adenocarcinoma cell growth were inhibited up to about 86%, in adding 1.0 ㎎/㎖ of extracts from HPE. This values were 9-12% higher than those from conventional water extraction. On in vivo experiment using ICR mice, the variation of body weight of mice group treated fresh ginseng extracts from HPE of 100 ㎎/㎏/day concentration was very lower than control and other group. The extracts from HPE was showed longer survival times as 35.65% than that of the control group, and showed the highest tumor inhibition activities compared with other group, which were 70.64% on Sarcoma-180 solid tumor cells. On the high performance liquid chromatogram (HPLC), amount of ginsenoside-Rg2, Rg3, Rh1 and Rh2 on fresh ginseng were increased up to 43-183% by HPE, compared with conventional water extracts. These data indicate that HPE definitely plays an important role in effectively extracting ginsenoside, which could result in improving anticancer activities. It can be concluded that low quality fresh ginseng associated with this process has more biologically compound and better anticancer activities than that from normal extraction process.
참고문헌 (38)
- Chang YK, "The expression of multidrug resistance-associated protein(MRP) and the chemosensitivity in gastric cancer cell lines", Journal of the Korean Surgical Society, 58, pp.752~759 (2000)
- Choi CH, "Reversal of P-glycoprotein-mediated multidrug resistance by protopanaxtriol ginsenoside from Korean red ginseng", Planta Medica, 69, pp.235~240 (2003)
- 최영진, "갑상선암 세포주에서 Na-4-Phenylbutyrate의 세포 증식 억제 및 분화 유도 효과", 대한외과학회지, 75(3), pp.162~170 (2008), 대한외과학회
- Doll, "The cause of cancer: quantitative estimates of avoidable risks of cancer in the United states today", Journal of the National Cancer Institute, 66, pp.1191~1308 (1983)
- Han CJ, "New treatments for hepatocellular carcinoma : Systemic chemotherapy", The Korean Journal of Hepatology, 14, pp.37~43 (2008)
- Han GD, "High pressure processing on foods", Food Industry and Nutrition, 10, pp.30~36 (2005)
- 한성태, "흑삼의 인삼 사포닌 분석", 약 학 회 지, 49(6), pp.490~494 (2005), 대한약학회
- Jang JW, "Review of currently used staging systems for hepatocellular carcinoma", of Korean Liver Cancer Study Group, 8, pp.19~23 (2008)
- 정향숙, "초고압 추출 처리에 의한 마황과 당귀의 항암 활성 증진", 한국약용작물학회지, 17(2), pp.102~108 (2009), 한국약용작물학회
- Jia WW, "Rh2, a compound extracted from ginseng, hypersensitizes multidrug-resistant tumor cells to chemotherapy", Canadian Journal of Physiology and Pharmacology, 82, pp.431~437 (2004)
- 김문일, "건강식품 C-22의 항암 효과", 생약학회지, 39(3), pp.237~240 (2008), 한국생약학회
- Kang SS, "Effect of ginseng on the expression of the onco-suppressor gene p21 in human breast-cancer cell lines", Journal of the Korean Surgical Society, 57, pp.782~788 (1999)
- Keum YS, "Inhibitory effects of the ginsenoside Rg3 on phorbol ester-induced cyclooxygenase-2 expression, NK- B activation and tumor promotion", Mutation Research, 523, pp.75~85 (2003)
- 김동청, "인삼의 지용성 추출물 투여가 면역기능에 미치는 영향", Journal of Ginseng Research, 32(1), pp.19~25 (2008), 고려인삼학회
- Kim DH, "The inhibitory effect of ginseng saponins on the stress-induced plasma interleukin-6 level in mice", Neuroscience Letters, 353, pp.13~16 (2003)
- 김은혜, "인삼의 항산화 작용", Journal of Ginseng Research, 33(1), pp.1~7 (2009), 고려인삼학회
- Kim HM, "Comparative studies of adriamycin and 28-deacetyl sendanin on in vitro growth inhibition of human cell lines", Archives of Pharmacal Research, 17, pp.100~103 (1994)
- 김홍태, "인진쑥 methanol 추출물의 투여가 암이 유발된 마우스에서 보인 혈액생화학적 및 종양 무게에 미치는 영향", 한국임상수의학회지, 24(3), pp.372~378 (2007), 한국임상수의학회
- 백남인, "식용 식물자원으로부터 활성물질의 탐색-XXIV.- 식용 식물 추출물의 항암 활성 -", 생약학회지, 38(4), pp.312~320 (2007), 한국생약학회
- 남기열, "홍삼과 백삼의 비교 고찰", Journal of Ginseng Research, 29(1), pp.1~18 (2005), 고려인삼학회
- Noh YH, "Ginsenoside-Rb2 and lovastatim on the expression of mRNAs for HNG CoA reductase and LDL receptor", Korean Journal of Ginseng Science, 20, pp.241~247 (1996)
- 이은주, "영양소 섭취 수준과 유방암 위험 - 대구·경북지역 환자-대조군 연구 -", 한국영양학회지, 41(8), pp.754~766 (2008), 한국영양학회
- Lee KY, "Ginsenoside-Rh2 blocks the cell cycle of SK-HEP-1 cells at the G1", S boundary by selectively inducing the protein expression of p27kip1, 1996, pp.110, 193~200
- 이미라, "백삼과 홍삼추출물의 학습수행과 Acetylcholinesterase 억제에 미치는 효과", Journal of Ginseng Research, 32(4), pp.341~346 (2008), 고려인삼학회
- 이재준, "지금초 추출물에 의한 TRAIL 저항성 인체위암세포의 세포사멸 유도", 생명과학회지, 18(1), pp.120~128 (2008), 한국생명과학회
- Lee SJ, "Antitumor activity of a novel ginseng saponin metabolite in human pulmonary adenocarcinoma cells resistant to cisplatin", Cancer Letters, 144, pp.39~43 (1999)
- Lee YJ, "A ginsenoside-Rh1, a component of ginseng saponin, activates estrogen receptor in human breast carcinoma MCF-7 cells", Journal of Steroid Biochemistry & Molecular Biology, 84, pp.463~468 (2003)
- Oh SH, "A ginseng saponin metabolite-induced apoptosis in HepG2 cells involves a mitochondria-mediated pathway and it downstream caspase-8 activation and Bid cleavage", Toxicology and Applied Pharmacology, 194, pp.221~229 (2004)
- Park JD, "Recent studies on the chemical constituents of Korean ginseng (Panax ginseng C.A. Meyer)", Journal of Ginseng Research, 20, pp.389~415 (1996)
- Schiller JH, "Comparison of four chemotherapy regimens for advanced non-small-cell lung cancer", New England Journal of Medicine, 346, pp.92~98 (2002)
- 손가현, "Platinum 제제가 포함된 항암화학요법을 받은 부인암 환자에서의 당뇨병 발생 위험", Korean Journal of Obstetrics and Gynecology, 51(2), pp.167~172 (2008), 대한산부인과학회
- 송용범, "홍삼물추출물이 고콜레스테롤 식이로 유도된 고콜레스테롤 혈증에서 간 기능 회복에 미치는 영향", Journal of Ginseng Research, 32(4), pp.283~290 (2008), 고려인삼학회
- Suh SO, "The effect of red ginseng for postoperative immune response in gastrointestinal carcinoma", Korean Journal of Ginseng Science, 22, pp.32~42 (1998)
- 서성옥, "진행성 위암 환자에서 수술 후 홍삼엑기스에 의한 면역 조절자 역할에 관한 전향적 연구", Journal of Ginseng Research, 28(2), pp.104~110 (2004), 고려인삼학회
- Xu TM, "Inhibitory effect of ginsenoside Rg3 combined with cyclophosphamide on growth and angiogenesis of ovarian cancer", Chinese Medical Journal, 120, pp.584~488 (2007)
- Yang JW, "Current status of processing and research trends in ginseng products", Journal of Ginseng Research, 20, pp.501~519 (1996)
- 윤이관, "암세포 특이적 세포 사멸을 유도하는 자생식물 추출물의 항암 효과", 생약학회지, 40(1), pp.6~12 (2009), 한국생약학회
- 윤탁, "진행성 비소세포폐암의 표적치료 및 맞춤항암치료", 대한내과학회지, 77(1), pp.9~17 (2009), 대한내과학회