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Inhibitory Effect of Panax notoginseng and Berberine on LPS-induced iNOS, COX-2 and Prostaglandin E2

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주제분류한의학

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Abstract

Many traditional herbal remedies exhibit several beneficial effects including anti-inflammation. Panax notoginseng Buck F.H. Chen. is used as a therapeutic agent to stop haemorrhages and a tonic to promote health in Korean and Chinese medicine. The pharmacokinetic profiles of the main P. notoginseng are still not accurately investigated. The exact mechanism of the anti-inflammatory action of P. notoginseng, however, has not been determined. In the present study, we examined the effect of P. notoginseng on lipopolysaccharide (LPS)-induced nitric oxide (NO) production, and inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene expression in RAW264.7 macrophages. The results indicated that P. notoginseng concentration-dependently inhibited LPS-induced NO production. Furthermore, P. notoginseng inhibited the expression of LPS-induced iNOS and COX-2 proteins without an appreciable cytotoxic effect on RAW264.7 cells. Berberine also inhibited LPS-induced iNOS protein as potently as P. notoginseng. This was consistent with the findings that P. notoginseng and also berberine inhibited prostaglandin E2 synthesis induced by LPS.

참고문헌 (29)

  1. A, "Macrophage oxidation of - arginine to nitrite and nitrate Nitric oxide is an intermediate", wish(k), pp.8706~8711 (1988)
  2. Nathan, "Nitric oxide as a secretory product of mammalian cells", pp.3051~3064 (1992)
  3. A, "Mammalian synthesis of nitrite", pp.249~257 (1988)
  4. D.L. Duval, "Characterization of hepatic nitric oxide synthase Identification as the cytokine-inducible form primarily regulated by oxidants", pp.277~284 (1996)
  5. T. Yuan, "Characterization of the Ca2+-dependent and -independent interactions between calmodulin and its binding domain of inducible nitric oxide synthase", pp.210~214 (1998)
  6. R.H. Liu, "Potential genotoxicity of chronically elevated nitric oxide", pp.73~89 (1995)
  7. A. Pedoto, "Treatment of septic shock in rats with nitric oxide synthase inhibitors and inhaled nitric oxide", pp.2021~2028 (1998)
  8. B. Tunctan, "Effects of nitric oxide synthase inhibition in lipopolysaccharide-induced sepsis in mice", pp.405~411 (1998)
  9. Ueno, "Endotoxin decreases the contractile responses of the porcine basilar artery to vasoactive substances", pp.712~719 (1993)
  10. C. Cao, "Induction by lipopolysaccharide of cyclooxygenase-2 mRNA in rat brain its possible role in the febrile response" (1995)
  11. N. Quan, "Cyclooxygenase 2 mRNA expression in rat brain after peripheral injection of lipopolysaccharide" (1998)
  12. Lee, "Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide", pp.2593~25938 (1992)
  13. Fujiwara, "Treatment trial of traditional oriental medicine in chronic viral hepatitis New Trends in Peptic Ulcer and Chronic Hepatitis", pp.141~146 (1987)
  14. Li, X.C, "Chinese herbal study on anti-ischemia- reperfusion-injury", pp.1~4 (1998)
  15. Zhang, G.Q.,, "Panax notoginseng saponins induced of human renal interstitial fibroblasts and its mechanisms Chinese Journal of Nephrology 14", pp.93~95 (1998)
  16. Haslam, "The role of plant polyphenols", mag(lato), pp.1~8 (1989)
  17. K. Aoki, "suppresses mouse mammary tumor gene expression via inhibition of poly", hata oe(thein b), pp.329~337 (1995)
  18. Takeuchi, "Inhibitory effects of flavonol glycosides on 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion", pp.774~776 (1990)
  19. Miyamoto, "Anti-tumor activity of oenothein B", hata, pp.99~103 (1993)
  20. Ojima, "Simultaneous determination of berberine palmatine and coptisine in crude drugs and oriental pharmaceutical preparations by ion-pair high-performance liquid chromatography", pp.354~357 (1982)
  21. H.S. Lee, "Narrowbore high performance liquid chromatography of berberine and palmatine in crude drugs and pharmaceuticals with ion-pair extraction using cobalt thiocyanate reagent", pp.59~63 (1999)
  22. H. Kim, "Chloromethyl ketones block induction of nitric oxide synthase in murine macrophages by preventing activation of nuclear factor-B", j immu(l 154), pp.4741~4748 (1995)
  23. Muller, "a mediator of lipopolysaccharide effects", immu(biology 187), pp.233~256 (1993)
  24. F. Yang, "Green tea polyphenols block endotoxin-induced tumor necrosis factor-production and lethality in a murine model", pp.2334~2340 (1998)
  25. J.K. Lin, "Cancer chemoprevention by tea polyphenols through mitotic signal transduction blockade", pp.911~915 (1998)
  26. Huang, "Immunosuppressive effect of Berberine", pp.359~364 (1992)
  27. Ma, L.Y, "Effects of saponins of Panax notoginseng on intracellular free Ca2+ concentration in dissociated neurons", pp.467~469 (1998)
  28. U.K. Meßmer, "Nitric oxide-induced apoptosis in RAW 264 7 macrophages is antagonized by protein kinase C- and protein kinase A-activating compounds", pp.757~765 (1995)
  29. U.K. Meßmer, "Nitric oxide-induced apoptosis in RAW 264 7 macrophages is antagonized by protein kinase C- and protein kinase A-activating compounds", pp.757~765 (1995)

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