갈근으로부터 추출한 β-sitosterol이 HEI-OC1 세포의 항산화 효소 활성에 미치는 영향
Effect of β-sitosterol from Pueraria thunbergiana on the Antioxidant Enzyme Activities in HEI-OC1 Cells
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Abstract
A mechanism of hair cell damage caused by noise and ototoxic agents is mediated through generation of free radicals and reactive oxygen species(ROS). It is known that most of animals have defense systems of ROS that protect against ROS, and the cochlea of animals also has ROS defense system, which appear efficient in detoxifying ROS generated under normal condition. This system includes several antioxidant enzymes such as superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase (GPX), and glutathione reductase(GR). The radix of Pueraria thunbergiana(P. thunbergiana) is traditionally prescribed to attenuate the clinical manifestation of inner ear dysfunction and various clinical situations including fevers, gastrointestinal disorders, skin problems, migraine headaches, lowering cholesterol, and treating chronic alcoholism in Oriental Medicine. In the present study, to investigate the protection mechanism of β-sitosterol from P. thunbergiana on cisplatin cytotoxicity toward HEI-OC1, we measured the effects of β-sitosterol on activities of SOD, CAT, GPX, and GR in cisplatin treated cells. SOD, CAT, GPX, and GR activities were significantly increased in the presence of 0.001-0.1 μg/ml of β-sitosterol compared to the control group. These results indicate that β-sitosterol protects cisplatin-induced HEI-OC1 cell damage through increasing the antioxidant enzyme system such as SOD, CAT, GPX, and GR.
참고문헌 (31)
- "Antioxidant enzyme levels asa function of growth state in cell culture", 19, pp.53~65 (1995)
- "Time course of changes in antioxidant enzymes in human skin fibroblasts after UVA irradiation", 14(3), pp.225~232 (1997)
- "from basic research to clinical application. The American Journal of Medicine 91" (1991)
- "Free radicals and antioxidants in the year 2000. A historical look to the future", 899, pp.136~147 (2000)
- "Reactive oxygen species in immune responses", 36(12), pp.1479~1480 (2004)
- "Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines", 271(18), pp.3646~3656 (2004)
- "Influence of Podophyllum hexandrum on endogenous antioxidant defence system in mice: possible role in radioprotection", 76(3), pp.253~262 (2001)
- "7761-7764", pp.1989~
- "Over expression of catalase in cytosolic or mitochondrial compartment protects HepG2 cells against oxidative injury", 274, pp.26217~26224 (1999)
- "Catalase-overexpressing thymocytes are resistant to glucocorticoid-induced apoptosis and exhibit increased net tumor growth", 61(6), pp.2766~2773 (2001)
- "9397-9400", pp.1994~
- "Effect of alpha-tocopherol succinate on free radical and lipid peroxidation levels in BL6 melanoma cells", 22(7), pp.1145~1151 (1997)
- "갈화와 갈근 열수추출물이 에탄올 투여 흰쥐의 혈청성분에 미치는 영향", 30(1), pp.92~96 (2001)
- "갈근이 뇌허혈 손상 흰쥐의 해마신경세포 손상에 미치는 영향", 19(1), pp.77~82 (2004)
- "갈근 에탄올 추출물이 흰쥐의 항산화계에 미치는 영향", 37(10), pp.872~880 (2004)
- "갈근추출물이 고지방식이를 섭취한 흰쥐의 지질대사에 미치는 영향", 28(1), pp.218~224 (1999)
- "갈근추출물이 에탄올을 투여한 흰쥐의 지질과산화에 미치는 영향", 28(4), pp.901~906 (1999)
- "갈근 추출물이 납을 투여한 흰쥐의 혈청 효소활성도 및 조직의 납 축적에 미치는 영향", 32(4), pp.914~919 (2000)
- "카드뮴을 급여한 흰쥐에서 갈근 열수 추출액의 해독작용효과", 17(4), pp.456~464 (2002)
- "A cochlear cell line as an in vitro system for drug ototoxicity screening", 8(4), pp.177~189 (2003)
- "K. Re-examination and further development of a precise and rapid dye method for measuring cell growth", cell kill. Journal of Immunological Methods 119, 1989., 203~210
- "Selective potentiation of gynecologic cancer cell growth in vitro by electromagnetic fields", 71(1), pp.64~71 (1998)
- "Superoxide produced by activated neutrophils efficiently reduces the tetrazolium salt, WST-1 to produce a soluble formazan: a simple colorimetric assay for measuring respiratory burst activation and for screening anti-inflammatory agents", 238(1-2), pp.59~68 (2000)
- "deprenyl induces activities of both superoxide dismutase and catalase but not of glutathione peroxidase in the striatum of young male rats. Life Sciences 48", (kubo), pp.517~521 (1991)
- "W.A. Assays of glutathione peroxidase. Methods in enzymology 105", pp.114~121 (1984)
- "B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. The Journal of Biological Chemistry 250", pp.5475~5480 (1975)
- "R.J. The Journal of Biological Chemistry 193", pp.265~275 (1951)
- "정세영. 천연성분을 이용한 cis-platin 신장독성 억제물질 개발 전통약물로부터 신약개발. 서울 경희대학교 경희공서약학연구소", pp.71~79 (1994)
- "Temporal bone histopathology of cisplatin ototoxicity", 16(6), pp.731~740 (1995)
- "Ototoxicity caused by cisplatin is ameliorated by melatonin and other antioxidants", 28(2), pp.73~80 (2000)
- "Up-regulation of adenosine receptors in the cochlea by cisplatin", 111(1-2), pp.143~152 (1997)