何首烏가 고혈압과 수축혈관에 미치는 영향
Effect of Aqueous Extract of Polygoni Multiflori Radix on Hypertension and Arterial Contraction in Animal Models
0
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
This research was aimed to examine the effect of Polygoni Multiflori Radix extract on the blood pressure in spontaneous hypertensive rat (SHR) and norepinephrine - induced arterial contraction in rabbit. In order to investigate the effect of Polygoni Multiflori Radix on rabbit's contracted vascular ring detached from common carotid artery, vascular ring with intact or damaged endothelium was used for the experiment using organ bath. To analyze the mechanism of Polygoni Multiflori Radix-induced relaxation, Polygoni Multiflori Radix extract was infused into contracted vascular ring which had been pretreated by Nω-nitro-L-arginine(L-NNA), Methylene blue(MB), and Ca2+ was infused into contracted vascular ring induced by NE after treatment of Polygoni Multiflori Radix extract in Ca2+-free solution. The results were as follows: Systolic blood pressure was significantly attenuated by administration of Polygoni Multiflori Radix. Blood flow and aldosterone were significantly decreased, but velocity and renin were not affected by Polygoni Multiflori Radix. Polygoni Multiflori Radix had an effective relaxation to the contracted vascular ring by NE in 0.03 mg/ml, 0.1 mg/ml and 0.3 mg/ml level. Polygoni Multiflori Radix had an effective relaxation to the intact endothelium vascular ring, but when endothelium was removed, vascular ring did not relax. Polygoni Multiflori Radix-induced relaxation was inhibited by the pretreatment of L-NNA and MB. Pretreatment of Polygoni Multiflori Radix extract inhibit the contraction by influx of extra-Ca2+ in contracted vascular ring induced by NE in Ca2+-free solution. As mentioned above, we suggest that Polygoni Multiflori Radix relaxes vascular ring through suppress influx of extra-cellular Ca2+ by the action of nitric oxide from endothelium.
Abstract
This research was aimed to examine the effect of Polygoni Multiflori Radix extract on the blood pressure in spontaneous hypertensive rat (SHR) and norepinephrine - induced arterial contraction in rabbit. In order to investigate the effect of Polygoni Multiflori Radix on rabbit's contracted vascular ring detached from common carotid artery, vascular ring with intact or damaged endothelium was used for the experiment using organ bath. To analyze the mechanism of Polygoni Multiflori Radix-induced relaxation, Polygoni Multiflori Radix extract was infused into contracted vascular ring which had been pretreated by Nω-nitro-L-arginine(L-NNA), Methylene blue(MB), and Ca2+ was infused into contracted vascular ring induced by NE after treatment of Polygoni Multiflori Radix extract in Ca2+-free solution. The results were as follows: Systolic blood pressure was significantly attenuated by administration of Polygoni Multiflori Radix. Blood flow and aldosterone were significantly decreased, but velocity and renin were not affected by Polygoni Multiflori Radix. Polygoni Multiflori Radix had an effective relaxation to the contracted vascular ring by NE in 0.03 mg/ml, 0.1 mg/ml and 0.3 mg/ml level. Polygoni Multiflori Radix had an effective relaxation to the intact endothelium vascular ring, but when endothelium was removed, vascular ring did not relax. Polygoni Multiflori Radix-induced relaxation was inhibited by the pretreatment of L-NNA and MB. Pretreatment of Polygoni Multiflori Radix extract inhibit the contraction by influx of extra-Ca2+ in contracted vascular ring induced by NE in Ca2+-free solution. As mentioned above, we suggest that Polygoni Multiflori Radix relaxes vascular ring through suppress influx of extra-cellular Ca2+ by the action of nitric oxide from endothelium.
참고문헌 (36)
- Franz, H, "Eberhard Ritz. Essential hypertension", Lancet, pp.370~603 (2007)
- Lee Goldman, "Cecil Textbook of Medicne", pp.346~350 (2004), United States of America, SAUNDERS
- 김종설, "고혈압의 이해와 치료", pp.10~13 (2002), 서울, 고려의학
- 해리슨내과학편찬위원회, "내과학", pp.1203~1205 (1997), 서울, 정담
- 조려화, "선학초 부탄올 추출물의 혈관 이완 효과의 기전에 대한 연구", 생약학회지, 37(2), pp.67~73 (2006), 한국생약학회
- 배명효, "山査의 내피세포 의존성 혈관이완효과 기전에 대한 연구", 大韓本草學會誌, 18(3), pp.169~180 (2003)
- 신형섭, "烏藥順氣散이 고혈압과 동맥혈관에 미치는 영향", 한국한의학연구원 논문집, 10(2), pp.79~92 (2004)
- 김대영, "祛風湯이 고혈압과 수축혈관에 미치는 영향", 한국한의학연구소 논문집, 8, pp.37~46 (2005)
- 全國韓醫科大學 本草學敎授, "本草學", pp.583~ (1998), 永林社
- 許浚, "東醫寶鑑", pp.15~1196 (2001), 서울, 한미의학
- 이영효, "何首烏가 고지혈증 흰쥐의 허혈성 뇌손상에 미치는 영향", 대한한의학회지, 26(3), pp.146~161 (2005), 대한한의학회
- R., Fattorusso, "Traditional Chinsese medicines with caspase-inhibitory activity", Phytomedicine, 13, pp.16~22 (2006)
- Liu, C, "Effect of the root of Polygonum multiflorum Thumb. and its processed products on fat accumulation in the liver of mice", Chung Kuo Chung yao Tsa Chih, 17(10), pp.595~596 (1992)
- Ching-Hsin Huang, "Chinsese herb Radix Polygoni Mutiflori as a therapeutic drug for live cirrhosis in mice", J. of Ethnopharmacology, 114, pp.199~206 (2007)
- 이원철, "赤何首烏(何首烏)가 고 cholesterol식이에 의하여 유발된 가토(家兎) 관상동맥이 죽상경화에 미치는 영향", 대한한의학회지, 16(1), pp.425~436 (1995)
- Yin, J.H, "Pharmacological and clinical studies on the processed products of radix Polygonum multiflori", Chung Kuo Chung yao Tsa Chih, 17(12), pp.722~724 (1992)
- 郑虎占, "中藥現代硏究與應用 卷三", pp.2319~2332 (1998), 學苑出版社
- 이영종, "何首烏가 고지혈증 흰쥐의 혈중 지질 및 효소활성에 미치는 영향", 대한본초학회지, 14(1), pp.69~77 (1999)
- 박원환, "내관족삼리혈의 何首烏약침이 고콜레스테롤혈증 병태 백서에 미치는 영향", 동의병리학회지, 14(1), pp.135~147 (2000)
- 옥은성, "何首烏추출물이 고혈당증 흰쥐에 미치는영향", 호남대학교논문집, 16, pp.261~267 (1998)
- 李濟馬, "東醫壽世保元", pp.73~ (1986), 杏林出版社
- Levi, R, "Therapies for perioperative hypertension. Phama- codynamic considerations. Acta Anaesthesiol", Scand. Suppl, 37(99), pp.16~19 (1993)
- Guyton, A.C, "Medical Physiology", W.B. Saunders Campany, 20, pp.230~243 (1986)
- Vanhoutte, P.M, "Platelets and vascular occlusion, Platelets, endothelium, and vasospasm", Circulation, 72(4), pp.728~734 (1985)
- Weir, M.R, "The renin- angiotensin - aldosterone system. A specific Target for hypertension management", Am. J. of Hypertension, 12, pp.205S~213S (1999)
- Furchgott, R.F, "The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine", Nature, 288, pp.373~376 (1980)
- Furchgott, R.F, "Evidence for endothelium-dependent vasodilation of resistance vessels by acetylcholine", Blood Vessels, 24, pp.145~149 (1987)
- Ignarro, L.J, "Pharmacological evidence that endothelium-derived relaxing factor is nitric oxide: use of pyrogallol and superoxide dismutase to study endothelium-dependent and nitric oxide-elicited vascular smooth muscle relaxation", J Pharmacol Exp Ther, 244, pp.181~189 (1988)
- Vanhoutte, P.M, "Modulation of vascular smooth muscle contraction by the endothelium", Ann. Rew. Physiol, 48, pp.307~320 (1986)
- Demirel, E, "TEA inhibits ACh-induced EDRF release: endothelial Ca2+-dependent K+-channels contribute to vascular tone", Am. J. Physiol. 267(heart Circ Physiol), 36, pp.H1135~H1141 (1994)
- Cheung, D.W, "Calcium activation of hyperpo- larization response to acetylcoholine in coronary endothelial cells", Journal of Cardiovascular Pharmacol, 20(12), pp.S120~S123 (1992)
- Johns, R.A, "Endothelium-derived relaxing factor. Basic review and clinical implications, J. Cardiothorac", Vasc. Anesth, 5(1), pp.69~79 (1991)
- Moore, P.K, "L-NG-nitro arginine(L-NOARG), a novel, L-arginine-reversible inhibitor of endothelium-dependent vasodilatation in vitro", Br. J. Pharmacol, 99, pp.408~412 (1990)
- Fabiato, A, "Calcium-induced release of calcium from the sarcoplasmic reticulum of skinned cells from adult human, drug, cat, rabbit, rat, and frog hearts and from fetal and new-born rat ventricles", pp.491~521 (1978), Annuals New York cademy of Sciences
- Cynthia Cauvin, "Cornelis van Breemen. Different Ca2+ Channels Along the arterial Tree", Journal of Cardio vascular Pharmacology. New York, 7(4), pp.4~10 (1985)
- 성호경, "생리학", pp.103~209 (1997), 서울. 의학문화사