복강 대식세포에서 피페린의 일산화질소, 인테루킨-10과 인테루킨-12의 억제 효과
Inhibitory Effects of Piperine on the Production of Nitric Oxide, Interleukin-10 and Interleukine-12 in Murine Peritoneal Macrophages
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The purpose of this study was to investigate the anti-inflammatory effects and cellular mechanism of piperine on murine peritoneal macrophages. To evaluate the effects of piperine, we examined the production of nitric oxide (NO), interleukin (IL)-10 and IL-12. To investigate inhibitory mechanism of piperine, we examined the MAPKs and Ik-Ba in murine peritoneal macrophages. Piperine itself does not have any cytotoxic effect and reduced lipopolysaccharid (LPS), Poly(I:C), CpG-ODN -induced production of NO, IL-10 and IL-12 in peritoneal macrophages. Piperine inhibited the activation of extracelluar signal-regulated kinase (ERK 1/2) and c-Jun NH2-terminal kinase (JNK 1/2) not the activation of p38 and the degradation of inhibitory kappa B a (Ik-Ba) in the LPS-stimulated murine peritoneal macrophages.In conclusion, Piperine down-regulated LPS-induced production of NO, IL-10 and IL-12, which could provide a clinical basis for anti-inflammatory properties of piperine.
Abstract
The purpose of this study was to investigate the anti-inflammatory effects and cellular mechanism of piperine on murine peritoneal macrophages. To evaluate the effects of piperine, we examined the production of nitric oxide (NO), interleukin (IL)-10 and IL-12. To investigate inhibitory mechanism of piperine, we examined the MAPKs and Ik-Ba in murine peritoneal macrophages. Piperine itself does not have any cytotoxic effect and reduced lipopolysaccharid (LPS), Poly(I:C), CpG-ODN -induced production of NO, IL-10 and IL-12 in peritoneal macrophages. Piperine inhibited the activation of extracelluar signal-regulated kinase (ERK 1/2) and c-Jun NH2-terminal kinase (JNK 1/2) not the activation of p38 and the degradation of inhibitory kappa B a (Ik-Ba) in the LPS-stimulated murine peritoneal macrophages.In conclusion, Piperine down-regulated LPS-induced production of NO, IL-10 and IL-12, which could provide a clinical basis for anti-inflammatory properties of piperine.
참고문헌 (26)
- Singh, Y.N, "Kava as over review", J. Ethnopharmacol, 37, pp.18~45 (1992)
- Srinivasan, K, "Black pepper and its pungent principle- piperine: a review of diverse physiological effects", Crit. Rev. Food Sci. Nutr, 47(8), pp.735~748 (2007)
- Pradeep, C.R, "Effect of Piperine on the Inhibition of Nitric Oxide (NO) and TNF-a Production", Imunopharmacology and Immunotoxicology, 25(3), pp.337~346 (2003)
- Kumar, S, "Piperine inhibits TNF-alpha induced adhesion of neutrophils to endothelial monolayer through suppression of NF-kappaB and IkappaB kinase activation", Eur. J. Pharmacol, 575(1), pp.117~186 (2007)
- Pradeep, C.R, "Piperine is a potent inhibitor of nuclear factor-kappaB (NF-kappaB), c-Fos, CREB, ATF-2 and proinflammatory cytokine gene expression in B16F-10 melanoma cells", Int. Immunopharmacol., 4(14), pp.1795~1803 (2004)
- Charles, A. Janeway, Jr, "Ruslan Medzhitov.Innate immune recognition", Annu. Rev. Immunol, 20, pp.197~216 (2002)
- Lopez-Bojorques, L.N, "Molecular mechanisms involved in the pathogenesis of septic shock", Arch. Med. Res, 35, pp.465~479 (2004)
- Nathan, C, "Nitric oxide synthases: roles, tolls and controls", Cell, 78, pp.915~918 (1994)
- Kwqamata, H, "Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW264.7 cells, a murine macrophage cell line", Am J Chin Med, 28, pp.217~226 (2000)
- Lee, B.G, "Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach", Eur J Pharmacol, 406, pp.301~309 (2000)
- Seo, W.G, "Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-and lipopolysaccharide", Gen Pharmacol, 35, pp.21~28 (2000)
- Chiou, W.F., "Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages", Life Sci, 69, pp.625~635 (2001)
- Horwood, N.J, "Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6, production", J Immunol, 176(6), pp.3635~3641 (2006)
- Hirohashi, N, "Low-dose lipopolysaccharid (LPS) pretreatment of mouse macrophage modulates LPS-dependent interleukin-6 production in vitro", Infect Immun, 64(3), pp.1011~ (1996)
- Matsuda, H, "Structural requirements of flavonoids for nitric oxide production inhibitory activity and mechanism of action", Bioorganic Med. Chem., 11, pp.1995~2000 (2003)
- Calixto, J.B, "Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion molecules", Planta Med, 70(2), pp.93~103 (2004)
- Atal, C.K, "Biochemical basis of enhanced drug bioavailability by piperine: evidence that piperine is a potent inhibitor of drug metabolism", J. Pharmacol. Exp. Ther, 232(1), pp.258~262 (1985)
- Shoba, G, "Influence of piperine on the pharmacokinetics of curcumin in animals and human volunteers", Planta Med, 64(4), pp.353~356 (1998)
- Mujumdar, A.M, "Anti-inflammatory activity of piperine", Jpn. J. Med. Sci. Biol, 43(3), pp.95~100 (1999)
- Hansson, G.K, "Toll to be paid at the gateway to the vessel wall", Arterioscler. Thromb. Vasc. Biol., 25(6), pp.1085~1087 (2005)
- Bhattacharyya, A, "Mitogen-activated protein kinases and NF-kappaB regulate H. pylori-mediated IL-8 release from macrophages", Biochem J., 366, pp.376~382 (2002)
- Binetruy, B, "Ha-Ras augments9 c-Jun activity and stimulates phosphoylation of its activation domain", Nature, 351, pp.122~127 (1991)
- Garrington, T.P, "Organization and regulation of mitogen-activated protein kinase signaling pathways", Curr Opin Cell Biol, 11, pp.211~218 (1999)
- Seo, J.H, "Helicobacter pylori in a Korean isolate activates mitogen-activated protein kinases. AP-1, and NF-kappaB and induces chemokine expression in gastric epithelial AGS cells", lab Invest, 84, pp.49~62 (2004)
- Lee, A.K, "Inhibition of lipopolysaccharide-inducible nitiric oxide synthase.TNF-α and COX-2 expression by suchinone effects on Iκ-Bα phosphorylation.C", EBP and AP-1 activation, 2003, pp.139, 11~20
- Meng, F, "Lipopolysaccharide(LPS)-induced macrophage activation and signal transduction in the absence of Src-family kinase Hck, Fgr, and Lyn", J Exp Med, 185(9), pp.1661~ (1997)