말초 신경병증성 통증 모델에서 소경활혈탕의 진통 효과
Analgesic Effects of Sokyungwhalhyul-tang on Constriction Nerve Injury-Induced Neuropathic Pain in Rats
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This study was conducted to determine the analgesic effect of Sokyungwhalhyul-tang(SKWHT) using the model of peripheral neuropathic pain model. A model of neuropathic pain was made by ligating left 5th lumbar spinal nerve of rats. After 1 days, the extract of SKWHT was orally administered daily. Rats were divided into four groups; (1) Control group(n=6), (2) Experimental group Ⅰ(SKWHT-OA1, 100 ㎎/㎏, n=6), (3) Experimental group Ⅱ(SKWHT-OA2, 300 ㎎/㎏, n=6), (4) Experimental group Ⅲ(SKWHT-OA3, 500 ㎎/㎏, n=6). After that, we examined the withdrawl response of neuropathic rats legs by von Frey filament and Hot plate at pre, 1^th, 4^th, 7^th, 14^th, 21^th days after the induction of neuropathic pain. And also we examined c-fos, GOT, GPT and histological study of Liver at 21^th days. von Frey filament and Hot plate were increase in experimental group Ⅰ,Ⅱ,Ⅲ than Con. especially group Ⅲ was most significantly analgesic effect than the other groups at 14th, 21^th days. In c-fos protein expression on spinal cord, group Ⅲ was most significantly reduction immunoreactivity at 21^th days and in blood serum GOT & GPT levels and histologic finding of Liver in all experimental groups were no significant difference with Con at 21^th days. According to the above results, SKWHT(500 ㎎/㎏) may have a significant analgesic effect on the neuropathic pain.
Abstract
This study was conducted to determine the analgesic effect of Sokyungwhalhyul-tang(SKWHT) using the model of peripheral neuropathic pain model. A model of neuropathic pain was made by ligating left 5th lumbar spinal nerve of rats. After 1 days, the extract of SKWHT was orally administered daily. Rats were divided into four groups; (1) Control group(n=6), (2) Experimental group Ⅰ(SKWHT-OA1, 100 ㎎/㎏, n=6), (3) Experimental group Ⅱ(SKWHT-OA2, 300 ㎎/㎏, n=6), (4) Experimental group Ⅲ(SKWHT-OA3, 500 ㎎/㎏, n=6). After that, we examined the withdrawl response of neuropathic rats legs by von Frey filament and Hot plate at pre, 1^th, 4^th, 7^th, 14^th, 21^th days after the induction of neuropathic pain. And also we examined c-fos, GOT, GPT and histological study of Liver at 21^th days. von Frey filament and Hot plate were increase in experimental group Ⅰ,Ⅱ,Ⅲ than Con. especially group Ⅲ was most significantly analgesic effect than the other groups at 14th, 21^th days. In c-fos protein expression on spinal cord, group Ⅲ was most significantly reduction immunoreactivity at 21^th days and in blood serum GOT & GPT levels and histologic finding of Liver in all experimental groups were no significant difference with Con at 21^th days. According to the above results, SKWHT(500 ㎎/㎏) may have a significant analgesic effect on the neuropathic pain.
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