황금 추출물의 신경병증성 통증 유발 흰쥐에 미치는 영향
Effects Study of Scutellariae Radix Extract on the Neuropathic Pain in Tibial and Common Peroneal Nerve Transected Rats
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
TRPA1 and TRPV1 are members of the TRP superfamily of structurally related, nonselective cation channels. TRPA1 and TRPV1 are often co-expressed in sensory neurons and play an important role in mechanical hyperalgesia and allodynia during neuropathic pain. Scutellariae Radix was reported to possess anti-inflammation properties and similar patterns of therapeutic action against different diseases. also Baicalin(a known principal constituent of Scutellaria Radix) was shown to down-regulate the mRNA expression levels of TRPV1. In this study, we observed the effects of Scutellariae Radix extract(SRE) in neuropathic pain induced SD rats via modulation of TRPV1 and TRPA1. Oral administration of a Scutellaria Radix extract(in doses of 300mg/kg, SRE(300)) showed a meaningful increase in the withdrawal threshold of mechanical allodynia and showed a meaningful decrease in the expression of c-fos compared to the control group. SRE(100) and SRE(300) showed a meaningful decrease in the expression of TRPV1 level compared to the control group. These results suggest that Scutellariae Radix extract could decrease mechanical allodynia by down-regulate the TRPV1 on the model of neuropathic pain.
Abstract
TRPA1 and TRPV1 are members of the TRP superfamily of structurally related, nonselective cation channels. TRPA1 and TRPV1 are often co-expressed in sensory neurons and play an important role in mechanical hyperalgesia and allodynia during neuropathic pain. Scutellariae Radix was reported to possess anti-inflammation properties and similar patterns of therapeutic action against different diseases. also Baicalin(a known principal constituent of Scutellaria Radix) was shown to down-regulate the mRNA expression levels of TRPV1. In this study, we observed the effects of Scutellariae Radix extract(SRE) in neuropathic pain induced SD rats via modulation of TRPV1 and TRPA1. Oral administration of a Scutellaria Radix extract(in doses of 300mg/kg, SRE(300)) showed a meaningful increase in the withdrawal threshold of mechanical allodynia and showed a meaningful decrease in the expression of c-fos compared to the control group. SRE(100) and SRE(300) showed a meaningful decrease in the expression of TRPV1 level compared to the control group. These results suggest that Scutellariae Radix extract could decrease mechanical allodynia by down-regulate the TRPV1 on the model of neuropathic pain.
참고문헌 (39)
- Jensen TS, "A new definition of neuropathic pain", Pain, 152, pp.2204~2205 (2011)[DOI]
- Maag R, "Neuropathic pain : translational research and impact for patient care", Curr Pain Headache Rep, 10(3), pp.191~198 (2006)[DOI]
- Devor M, "Nerve pathophysiology and mechanisms of pain in causalgia", J Auton Nerv syst, 7, pp.371~384 (1983)[DOI]
- Bonica JJ, "Causalgia and other reflex sympathetic dystrophies. The management of pain", pp.220~243 (1990), Lea and Febiger Publishers
- R. Baron, "Mechanisms of disease: neuropathic pain. A clinical perspective", Nature Clinical Practice Neurology, 2(2), pp.95~106 (2006)[DOI]
- Caterina MJ, "The capsaicin receptor: a heatactivated ion channel in the pain pathway", Nature, 389, pp.816~824 (1997)[DOI]
- Jung SJ, "The Role of Transient Receptor Potential Channel in Pain", hanyang medical reviews, 31(2), pp.116~122 (2011)[DOI]
- Vellani V, "Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones", Mol Pain, 6, pp.61~ (2010)
- Story GM, "ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures", Cell, 112, pp.819~829 (2003)[DOI]
- Kim, HC., "Textbook of herbal pharmacology", pp.129~133 (2001), Jipmundang
- SB Yoon, "Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-Activated RAW264,7 Macrophages", J Ethnophar, 125, pp.286~290 (2009)[DOI]
- Choi J, "Flavones from Scutellaria baicalensis Georgi attenuate apoptosis and protein oxidation in neuronal cell lines", Biochimica et Biophysica Acta, 1571, pp.201~210 (2002)[DOI]
- 양기영, "척수압박손상 흰쥐의 척수조직 염증반응에 黃芩이 미치는 영향", 한방재활의학과학회지, 21(3), pp.1~11 (2011), 한방재활의학과학회
- 김은석, "黃芩이 척수압박손상 흰쥐의 운동기능장애에 미치는 영향", Journal of Acupuncture Research, 28(3), pp.33~42 (2011), 대한침구의학회
- Sui F, "Anti-nociceptive mechanism of baicalin involved in intervention of TRPV1 in DRG neurons in vitro", Journal of Ethnopharmacology, 129, pp.361~366 (2010)[DOI]
- Butera JA, "Current and emerging targets to treat neuropathic pain", J Med Chem, 50(11), pp.2543~2546 (2007)[DOI]
- Nickel FT, "Mechanisms of neuropathic pain", Eur Neuropsychopharmacology, 22, pp.81~91 (2012)[DOI]
- Yun DM, "Neuropathic pain", The Korean Journal of Pain, 8(2), pp.219~224 (1995)
- Honore PH, "Neuropathic pain models in the development of analgesic drugs", Scandinavian Journal of Pain, 2(4), pp.172~177 (2011)
- Bennett GJ, "A peripheral mononeuropathy in rats that produces disorders of pain sensation like those seen in man", Pain, 33, pp.87~107 (1988)[DOI]
- Seltzer Z, "A novel behavioral model of neuropathic pain disorders produced in rats by partial sciatic nerve injury", Pain, 43, pp.205~218 (1990)[DOI]
- Kim SH, "An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat", Pain, 50, pp.355~363 (1992)[DOI]
- Decosterd I, "Spared nerve injury: an animal model of persistent peripheral neuropathic pain", Pain, 87, pp.149~158 (2000)[DOI]
- Dowdall T, "Comparison of five different rat models of peripheral nerve injury", Pharmacol Biochem Behav, 80(1), pp.93~108 (2005)[DOI]
- E. Palazzo, "Moving towards supraspinal TRPV1 receptors for chronic pain relief", Molecular Pain, 6 (2010)
- Walker KM, "The VR1 antagonist capsazepine reverses mechanical hyperalgesia in models of inflammatory and neuropathic pain", Journal of Pharmacology and Experimental Therapeutics, 304(1), pp.56~62 (2003)[DOI]
- Wan HY, "Effect of traditional Chinese medicines with different properties on thermoregulation and temperature-sensitive transient receptor potentialion channel protein of rats with yeast-induced fever", China Journal of Chinese Materia Medica, 39(19), pp.3813~3818 (2014)
- 임병우, "Inhibitory Effect of Scutellaria baicalensis Root Extract on ChemicalMediator Release and Immune Response", 한국약용작물학회지, 15(2), pp.132~137 (2007), 한국약용작물학회
- R.M. Levy, "A Medicinal Extract of Scutellaria baicalensis and Acacia catechu Acts as a Dual Inhibitor of Cyclooxygenase and 5-Lipoxygenase to Reduce Inflammation", Journal of Medicinal Food, 10(3), pp.442~451 (2007), 한국식품영양과학회
- Gao, Z, "Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi", Biochim. Biophys. Acta., 1472, pp.643~650 (1999)[DOI]
- Huang, Y, "Biological properties of baicalein in cardiovascular system", Curr Drug Targets Cardiovasc Haematol Disord, 5, pp.177~184 (2005)[DOI]
- Tu XK, "Neuroparotective effect of baicalin in a rat model of permanent focal cerebral ishemia", Neurochem Res, 34(9), pp.1626~1634 (2009)[DOI]
- 반효정, "黃芩이 척수압박손상 흰쥐의 척수신경세포 자연사에 미치는 영향", 한방재활의학과학회지, 21(4), pp.13~22 (2011), 한방재활의학과학회
- Jongen JL, "Neuropathic pain and pharmacological treatment", Pain Pract, 14(3), pp.283~295 (2014)[DOI]
- Barber J, "Examining the use of tramadol hydrochloride as an antidepressant", Exp Clin Psychopharmacol, 19(2), pp.123~130 (2011)[DOI]
- Heavner JE, "Thermal grill illusion and complex regional pain syndrome type I(reflex sympathetic dystrophy)", Reg Anesth., 22(3), pp.257~259 (1997)[DOI]
- Wahren LK, "Quantitative sensory testing before and after regional guanethidine block in patients with neuralgia in the hand", Pain, 46(1), pp.23~30 (1991)[DOI]
- Harris JA, "Using c-fos as a neural marker of pain", Brain Res Bull, 45(1), pp.1~8 (1998)[DOI]
- Coggeshall RE, "Fos, nociception and the dorsal horn", Prog Neurobiol, 77(5), pp.299~352 (2005)