광혈전 유도 뇌손상 백서에서 疏風湯과 tDCS의 적용이 운동기능회복과 GDNF 발현에 미치는 영향
Effect of Sopung-tang and tDCS on Motor function Recovery and GDNF Expression in Photothrombotic Brain Infarction Induced Rat Model
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he effect of Sopung-tang(SPT) and trans-cranial direct current stimulation(tDCS) was investigated in photothrombotic brain infarction(PTI) rats. Sprague-Dawley 80 rats, were divided into four groups. group Ⅰ was experiental control group(n=20), group Ⅱ was PTI induced and oral administration of SPT(n=20), group Ⅲ was PTI induced and tDCS administration(n=20) and group Ⅳ was PTI induced and SPT and tDCS administration for 28 days on stroke rats(n=20). Analysis the neurological function test, 25 point behavior functional score test, and immunohistochemistric finding of GDNF expression, and electron microscopy assessment In motor behavior test, the outcome of group Ⅳ was significantly difference than the other group, and In immunohistochemistric finding, group Ⅱ, Ⅲ, Ⅳ were increase GDNF expression on 28 days, In electron microscopy finding, the all groups were degenerated of cell organelles, and synaptic plasticity were improvement of group Ⅱ, Ⅲ, Ⅳ(especially group Ⅳ) These results suggest that, 28days application of SPT and tDCS was the motor function and histopathologic, micro-morphological improvement of motor function recovery and positive influence on synaptic plasticity
Abstract
he effect of Sopung-tang(SPT) and trans-cranial direct current stimulation(tDCS) was investigated in photothrombotic brain infarction(PTI) rats. Sprague-Dawley 80 rats, were divided into four groups. group Ⅰ was experiental control group(n=20), group Ⅱ was PTI induced and oral administration of SPT(n=20), group Ⅲ was PTI induced and tDCS administration(n=20) and group Ⅳ was PTI induced and SPT and tDCS administration for 28 days on stroke rats(n=20). Analysis the neurological function test, 25 point behavior functional score test, and immunohistochemistric finding of GDNF expression, and electron microscopy assessment In motor behavior test, the outcome of group Ⅳ was significantly difference than the other group, and In immunohistochemistric finding, group Ⅱ, Ⅲ, Ⅳ were increase GDNF expression on 28 days, In electron microscopy finding, the all groups were degenerated of cell organelles, and synaptic plasticity were improvement of group Ⅱ, Ⅲ, Ⅳ(especially group Ⅳ) These results suggest that, 28days application of SPT and tDCS was the motor function and histopathologic, micro-morphological improvement of motor function recovery and positive influence on synaptic plasticity
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