본문 바로가기
KERD
논문KCI 등재

용담초 추출물의 화장품 소재로서 약리활성에 관한 연구

Study on Pharmacological Activation as Cosmetic Material of Gentianae scabrae bunge Extract

1

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류한의학

초록

In this study, we investigated the effect of ethanol extracts from Gentianae scabrae bunge (GSB) on the activities of antioxidant, whitening and anti-inflammation. Viability of cells was measured by neutral red (NR) assay, and inhibitory effects of GSB on melanin synthesis was determined the melanin production in B16F10 cells. The expression level of matrix metalloproteinase-1 (MMP-1) in media was analyzed by ELISA kit, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. GSB showed highly efficacy in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and significantly reduced melanin synthesis in B16F10 cells. MMP-1 production in UVB-stimulated human dermal fibroblast (HDF) cells was inhibited by GSB treatments. NO production was suppressed by the treatment of GSB in LPS-stimulated RAW264.7 cells. From this results, it was indicated that GSB could be utilized as anti-aging and whitening cosmetic ingredients.

Abstract

In this study, we investigated the effect of ethanol extracts from Gentianae scabrae bunge (GSB) on the activities of antioxidant, whitening and anti-inflammation. Viability of cells was measured by neutral red (NR) assay, and inhibitory effects of GSB on melanin synthesis was determined the melanin production in B16F10 cells. The expression level of matrix metalloproteinase-1 (MMP-1) in media was analyzed by ELISA kit, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. GSB showed highly efficacy in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and significantly reduced melanin synthesis in B16F10 cells. MMP-1 production in UVB-stimulated human dermal fibroblast (HDF) cells was inhibited by GSB treatments. NO production was suppressed by the treatment of GSB in LPS-stimulated RAW264.7 cells. From this results, it was indicated that GSB could be utilized as anti-aging and whitening cosmetic ingredients.

참고문헌 (40)

  1. Lim, D. K., "Antioxidative activity of ethanol extract from Korean medicinal plants", Korean J. Food Sci.Technol, 28(1), pp.83~89 (1996)
  2. Kim, H. K., "Antioxidative activity and physiological activity of some Korean medicinal plants", Korean J. Food Sci, Technol, 27(1), pp.80~85 (1995)
  3. Kang, T. C., "Human histology", Ko Moon Sa, pp.263~ (2012)
  4. Kubo, M., "Development tudies of cuticle and medicinal drug from natural sources on melanin biosynthesis", Fragrance J, 8, pp.48~55 (1995)
  5. Do, J. R., "Selection of beauty material from Fruits and Vegetables", J Kor Soc Food Sci. Nutr, 10, pp.248~248 (2008)
  6. 유인식, "싸리꽃 에탄올 추출물의 皮膚 生理活性 효과", 한방안이비인후피부과학회지, 20(2), pp.1~9 (2007), 대한한방안이비인후피부과학회
  7. Kim, H.C., "Pharmacology for Herbal Medicine", pp.140~141 (2008), Jipmoondang
  8. Lee, H. J., "Screening of Inhibitor of Thyroid Peroxidase, and Oxidative Coupling Enzyme from Natural Poducts", 43(3), pp.334~341 (1999)
  9. Seo, J.W., "The anti-oxidative activities and separation of the Gentiana scabra var. buergeri extracts", pp.2008
  10. 김미선, "龍膽草 추출물이 LPS로 활성화된 Raw 264.7 cell에서의 pro-inflammatory mediator에 미치는 영향", 한방안이비인후피부과학회지, 21(2), pp.28~38 (2008), 대한한방안이비인후피부과학회
  11. Han, S.W., "Effects of Aqua - acupuncture with Gentianae scabrae Radix and Bupleuri Radix Extract on Serum Activities of GOT, GPT, LDH, ALP and r-GTP in Damaged Rat Liver induced by the Carbon Tetracholoride (CCL4).", The Acupuncture, 10(1), pp.297~313 (1993)
  12. Moon, S. H., "Effects of G.S.R. Aqua - acupuncture by Density Gallstone Formation im Mice", J Kor Acupuncture & Moxibustion Soc, 8(1), pp.141~156 (1991)
  13. Blois, M.S., "Anti oxidant deter minations by the use of stable free radical", Nature, 18, pp.1199~1120 (1958)
  14. Marklund, S., "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J Biochem, 47(3), pp.469~474 (1974)
  15. Borenfreund, E., "Toxicity determined in vitro by morphological alterations and neutral red absorption", Toxicol Lett, 24(2-3), pp.119~124 (1984)
  16. 임혜원, "B16 Melanoma세포에서 Citrus Essential Oil 이 Melanin 생성에 미치는 영향", 약 학 회 지, 47(1), pp.25~30 (2003), 대한약학회
  17. Green strok, C.L, "Radiation and aging free radical damage, biological response and possible antioxidant intervention", Med Hypotheses, 41(5), pp.473~482 (1993)
  18. Kim, K. H, "A Study on the Application of Cosmetic Materials using Antioxidant Activity of the Extracts from Castanea crenata Leaf", pp.2011
  19. Pryor, W.A., "Oxy-radicals and related specied their formation life-time and reactions", Annu Rev Physiol, 48, pp.657~667 (1986)
  20. Homebeck, W., "Downregulation of tissue inhibitor of matrix metallo protease-1 (TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival", J Pathol Biol, 51(10), pp.569~ (2003)
  21. Papa, S., "Reactive oxygen species, mitochondria, apoptosis and aging", Mol Cell Biol, 174(1-2), pp.305~319 (1997)
  22. W. laschej, M., "Scharffetter-Kochanek K. Singlet oxygen may mediate the ultraviolet A-induced synthesis of interstitial collagenase", J.Invest.Dermatol, 104(2), pp.194~198 (1995)
  23. Jang, M.J., "Studies on anti-oxidant of Pinus rigida inner bark for the development of functional cosmetics", pp.2007
  24. Kitani, K., "Pharmacological interventions in aging and age-associated disorders: potentials of propargylamines for human use", Ann. N.Y. Acad Sci, 959, pp.295~307 (2002)
  25. Kubo, M., "Development tudies of cuticle and medicinal drug from natural sources on melanin biosynthesis", Fragrance J, 8, pp.48~55 (1995)
  26. Do, J. R., "Selection of beauty material from Fruits and Vegetables", J Kor Soc Food Sci. Nutr, 10, pp.248~248 (2008)
  27. Lee, S.J., "Novel natural products as active material for beauty food", Food Sci Ind, 40, pp.10~18 (2008)
  28. Zague, V., "A new view concerning the effects of collagen hydroly- sate intake on skin properties", Arch Dermatol Res, 300, pp.479~483 (2008)
  29. Kim, N. Y., "Anitric oxide synthesis inhibitor from the roots of Gentiana scabra in RAW 264.7 cells", J Kor Pharmacogn, 30(2), pp.173~176 (1999)
  30. Zhu, Y.P., "Harwood academic publishers", pp.146~149 (1998), Chin. Mat. Med
  31. Huh, H., "PAF antagonistic activity of 2-hydroxy -3-methoxybenzoic acid glucose ester from Genyiana scabra", Arch Pharm, 21(4), pp.436~439 (1988)
  32. Willoughby, D.A., "Human arthritis applied to animal models. Towards a better therapy", Ann Rheum Dis, 34, pp.471~478 (1975)
  33. Chung, E. K., "Anti-inflammaatory and anti-allergic effect of extracts from organic soybean", Korean J Organic Agric, 2, pp.245~253 (2011)
  34. Garthwaite, J., "New insight into the functioning of nitric oxidereceptive guanylyl cyclase: physiological and pharmacological implications", Mol. Cell Biochem, 334, pp.221~232 (2010)
  35. Förstermann, U., "Nitric oxide synthases: regulation and function", Eur. Heart J, 33, pp.829~837 (2012)
  36. Alderton, W. K., "Nitric oxide synthases: structure, function and inhibition", Biochem J, 357, pp.593~615 (2001)
  37. Andrew, P. J., "Enzymatic function of nitric oxide synthases", Cardiovasc. Res, 43, pp.521~531 (1999)
  38. De Cruz, S. J., "Bench-to-bedside review: the role of nitric oxide in sepsis.", Expert Rev. Respir. Med, 3, pp.511~521 (2009)
  39. Nathan, C. F., "Role of nitric oxide synthesis in macrophage antimicrobial activity", Curr Opinion Immunol, 3(1), pp.70~ (1991)
  40. 이보한, "간경변증 환자에서 말초혈액 Nitric Oxide 활성도 측정의 의의", 대한내과학회지, 73(3), pp.251~257 (2007), 대한내과학회

관련 논문AI추천

이 간행물의 다른 논문 (8건)