Favorable Hepatoprotective Effects of Gongjin-dan on the Acute Ethanol-induced Liver Damaged C57BL/6 Mice
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
To observe the potential hepatoprotective effects of Gongjin-dan on the acute ethanol (EtOH)-induced liver damages in C57BL/6 mice with its possible action mechanisms. EtOH-mediated acute hepatic damages were induced by oral administration of EtOH total 3 doses. The changes on the body weight, liver weight, albumin, TG, AST, ALP, ALT, hepatic TG contents, hepatic antioxidant defense system, TNF-α, CYP 2E1 activity and mRNA expressions of hepatic lipogenic genes - SREBP-1c, SCD1, ACC1, FAS, PPARγ and DGAT2 or genes involved in fatty acid oxidation - PPARα, ACO and CPT1 were observed with final liver histopathological inspections after 15 days of continuous administration of silymarin 200 mg/kg, Gongjin-dan (GJD) 400, 200 and 100 mg/kg. The results were compared with silymarin 200 mg/kg treated mice. Marked decreases of body and liver weights, increases of serum AST, ALT, Albumin and TG levels, hepatic TG contents, TNF-α level, CYP 2E1 activity and mRNA expressions of hepatic lipogenic genes or decreases mRNA expressions of genes involved in fatty acid oxidation were observed with histopathological changes related hepatosteatosis increases of immunolabelled hepatocytes, as the results of a binge drinking of EtOH in the present study. Also destroys of hepatic antioxidant defense systems were demonstrated in EtOH control mice as compared with intact vehicle control mice, respectively. The results suggest that oral administration of 400, 200 and 100 mg/kg of GJD favorably protected the liver damages from acute mouse EtOH intoxications.
Abstract
To observe the potential hepatoprotective effects of Gongjin-dan on the acute ethanol (EtOH)-induced liver damages in C57BL/6 mice with its possible action mechanisms. EtOH-mediated acute hepatic damages were induced by oral administration of EtOH total 3 doses. The changes on the body weight, liver weight, albumin, TG, AST, ALP, ALT, hepatic TG contents, hepatic antioxidant defense system, TNF-α, CYP 2E1 activity and mRNA expressions of hepatic lipogenic genes - SREBP-1c, SCD1, ACC1, FAS, PPARγ and DGAT2 or genes involved in fatty acid oxidation - PPARα, ACO and CPT1 were observed with final liver histopathological inspections after 15 days of continuous administration of silymarin 200 mg/kg, Gongjin-dan (GJD) 400, 200 and 100 mg/kg. The results were compared with silymarin 200 mg/kg treated mice. Marked decreases of body and liver weights, increases of serum AST, ALT, Albumin and TG levels, hepatic TG contents, TNF-α level, CYP 2E1 activity and mRNA expressions of hepatic lipogenic genes or decreases mRNA expressions of genes involved in fatty acid oxidation were observed with histopathological changes related hepatosteatosis increases of immunolabelled hepatocytes, as the results of a binge drinking of EtOH in the present study. Also destroys of hepatic antioxidant defense systems were demonstrated in EtOH control mice as compared with intact vehicle control mice, respectively. The results suggest that oral administration of 400, 200 and 100 mg/kg of GJD favorably protected the liver damages from acute mouse EtOH intoxications.
참고문헌 (35)
- Meyer, S. A., "Introduction to biochemical toxicology", pp.487~490 (2001), John Wiley & Sons
- Nordmann, R., "Alcohol and antioxidant systems", Alcohol Alcohol, 29, pp.513~522 (1994)
- Husain, K., "Chronic ethanol and nicotine interaction on rat tissue antioxidant defense system", Alcohol, 25, pp.89~97 (2001)
- DeLeve, L. D., "Glutathione metabolism and its role in hepatotoxicity", Pharmacol Ther, 52, pp.287~305 (1991)
- Yang, P., "Endogenous A1 adenosine receptor protects mice from acute ethanol-induced hepatotoxicity", Toxicology, 309, pp.100~106 (2013)
- Song, Z., "Silymarin protects against acute ethanol-induced hepatotoxicity in mice", Alcohol Clin Exp Res, 30(3), pp.407~413 (2006)
- Ghoneim, A. I., "Anti-apoptotic effects of tamarind leaves against ethanol-induced rat liver injury", J Pharm Pharmacol, 64, pp.430~438 (2012)
- Kim, Y. H., "The effect of Kong Jin Dan on the lipid metabolism in rats fed high fat-diet", J. East-West Med, 14, pp.61~78 (2001)
- 허현, "Hepatoprotective effects of GongJinDan, on ethanol-mediated experimental liver damage in rats", 박사, 내과학(한의학), pp.2008
- Butler, W. M., "The direct determination of liver triglycerides", J Lipid Res, 2, pp.95~96 (1961)
- Bucolo, G., "Quantitative determination of serum triglycerides by the use of enzymes", Clin Chem, 19, pp.476~482 (1973)
- Lowry, O. H., "Protein measurement with the Folin phenol reagent", J Biol Chem, 193, pp.265~275 (1951)
- Kavutcu, M., "Reduced enzymatic antioxidant defense mechanism in kidney tissues from gentamicin-treated guinea pigs: effects of vitamins E and C", Nephron, 72, pp.269~274 (1996)
- Jamall, I. S., "Effects of cadmium on glutathione peroxidase, superoxidase dismutase and lipid peroxidation in the rat heart: a possible mechanism of cadmium cardiotoxicity", Toxicol Appl Pharmacol, 80, pp.33~42 (1985)
- Sedlak, J., "Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent", Anal Biochem, 25, pp.192~205 (1968)
- Aebi, H., "Methods in Enzymatic Analysis", pp.673~686 (1974), Academic Press
- Sun, Y., "A simple method for clinical assay of superoxide dismutase", Clin Chem, 34, pp.497~500 (1988)
- Jung, Y. M., "Fermented garlic protects diabetic, obese mice when fed a high-fat diet by antioxidant effects", Nutr Res, 31, pp.387~396 (2011)
- 기성환, "Red ginseng extract protects against carbon tetrachloride-induced liver fibrosis", Journal of Ginseng Research, 37(1), pp.45~53 (2013), 고려인삼학회
- Chen, Y. H., "C", EBP beta and C, Acta Pharmacol Sin, pp.2009, 30[DOI]
- Levene, A., "Pathological factors influencing excision of tumours in the head and neck. Part I", Clin Otalary, 6, pp.145~151 (1981)
- Ludbrook, J., "Update: microcomputer statistics packages. A personal view", Clin Exp Pharmacol Physiol, 24, pp.294~296 (1997)
- Pacher, P., "Nitric oxide and peroxynitrite in health and disease", Physiol Rev., 87, pp.315~424 (2007)
- Smathers, R. L., "Overview of lipid peroxidation products and hepatic protein modification in alcoholic liver disease", Chem Biol Interact, 192(1-2), pp.107~112 (2011)
- Li, Y. M., "Effect of acute alcoholism on hepatic enzymes and oxidation", antioxidation in rats, 2004, pp.3, 241~244
- Das, S. K., "Time-dependent effects of ethanol on blood oxidative stress parameters and cytokines", Indian J Biochem Biophys, 46, pp.116~121 (2009)
- Ho, W. Y., "Hepatoprotective activity of Elephantopus scaber on alcohol-induced liver damage in mice", Evid Based Complement Alternat Med, 2012, pp.417953~ (2012)
- Zhou, Z., "Metallothionein protection against alcoholic liver injury through inhibition of oxidative stress", Exp Biol Med, 227, pp.214~222 (2002)
- McCarty, M. F., "Inhibition of CYP 2E1 with natural agents may be a feasible strategy for minimizing the hepatotoxicity of ethanol", Med Hypotheses, 56, pp.8~11 (2001)
- Navasumrit, P., "Ethanol-induced free radicals and hepatic DNA strand breaks are prevented in vivo by antioxidants: effects of acute and chronic ethanol exposure", Carcinogenesis, 21, pp.93~99 (2000)
- Messarah, M., "Influence of thyroid dysfunction on liver lipid peroxidation and antioxidant status in experimental rats", Exp Toxicol Pathol, 62, pp.301~310 (2010)
- Wada, S., "Fish oil fed prior to ethanol administration prevents acute ethanol-induced fatty liver in mice", J Hepatol, 49, pp.441~450 (2008)
- Dey, A., "Alcohol and oxidative liver injury", Hepatology, 43, pp.S63~S74 (2006)
- Leung, T. M., "Argininosuccinate synthase conditions the response to acute and chronic ethanol-induced liver injury in mice", Hepatology, 55, pp.1596~1609 (2012)
- Galligan, J. J., "Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease", J Toxicol, 2012, pp.207594~ (2012)