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淸肝解酒湯의 항산화 작용에 관한 실험적 연구

Experimental Study of Chungganhaeju-tang (Qingganjiejiu-tang) on Oxidative Stress

이지은|이장훈
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2011.06|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제32권 제2호|pp. 188-202

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참고문헌

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CYP2E1oxidative stressethanolChungganhaeju-tang (Qingganjiejiu-tang)
주제분류한의학

초록

Objectives : Oxidative stress seems to play a major role in mechanisms by which ethanol causes liver injury. Previous studies have shown that treatment with Chungganhaeju-tang (Qingganjiejiu-tang, CGHJT) has protective effects on alcoholic liver disease. The aim of this study was to investigate the effects of Chungganhaeju-tang on oxidative stress. Materials and Methods : In vitro, we evaluated the inhibitory activities of CGHJT on DPPH (1,1-diphenyl-2- picryl-hydrazyl), xanthine oxidase, trypsin, and hyaluronidase, and measured cell viability, and proliferation. In the cell culture model, we measured the activities of superoxide dismutase (SOD), and catalase (CAT) after CGHJT treatment in C34 and E47 cell lines, HepG2 cells transfected with/without the cytochrome P450 2E1 (CYP2E1) gene. In vivo, we measured malondialdehyde levels in the liver tissue and alcohol concentration in the blood. Results : CGHJT showed significant free radical scavenging activity against DPPH and xanthine oxidase in the in vitro study, and increased cell viability, proliferation, and activities of superoxide dismutase, catalase in C34 and in E47 cell lines. CGHJT reduced malondialdehyde levels and blood alcohol concentration in vivo, as well. Conclusions : This study suggests that CGHJT has antioxidant effects on oxidative stress by reducing lipid peroxidation and inhibiting the ethanol induced suppression of antioxidant enzyme activities.

Abstract

Objectives : Oxidative stress seems to play a major role in mechanisms by which ethanol causes liver injury. Previous studies have shown that treatment with Chungganhaeju-tang (Qingganjiejiu-tang, CGHJT) has protective effects on alcoholic liver disease. The aim of this study was to investigate the effects of Chungganhaeju-tang on oxidative stress. Materials and Methods : In vitro, we evaluated the inhibitory activities of CGHJT on DPPH (1,1-diphenyl-2- picryl-hydrazyl), xanthine oxidase, trypsin, and hyaluronidase, and measured cell viability, and proliferation. In the cell culture model, we measured the activities of superoxide dismutase (SOD), and catalase (CAT) after CGHJT treatment in C34 and E47 cell lines, HepG2 cells transfected with/without the cytochrome P450 2E1 (CYP2E1) gene. In vivo, we measured malondialdehyde levels in the liver tissue and alcohol concentration in the blood. Results : CGHJT showed significant free radical scavenging activity against DPPH and xanthine oxidase in the in vitro study, and increased cell viability, proliferation, and activities of superoxide dismutase, catalase in C34 and in E47 cell lines. CGHJT reduced malondialdehyde levels and blood alcohol concentration in vivo, as well. Conclusions : This study suggests that CGHJT has antioxidant effects on oxidative stress by reducing lipid peroxidation and inhibiting the ethanol induced suppression of antioxidant enzyme activities.

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茵蔯淸肝湯의 알코올성 산화스트레스에 대한 보호효과 연구김영태, 우홍정대한한방내과학회 · 대한한방내과학회지2011 · 제32권 제4호淸肝解酒湯이 알코올 유발 간섬유화와 단백질 발현에 미치는 영향김영철, 우홍정, 이장훈, 전재현대한한방내과학회 · 대한한방내과학회지2008 · 제29권 제2호淸肝解酒湯이 CYP2E1-transfected HepG2 cell에서 알코올유발 세포독성에 미치는 영향김영철, 우홍정, 이장훈, 이지은대한한방내과학회 · 대한한방내과학회지2006 · 제27권 제1호柴苓湯이 에탄올 투여로 유발된 흰쥐의 간손상에 미치는 방어효과김범회, 최영현한의병리학회 · 동의생리병리학회지2013 · 제27권 제5호과산화수소로 유도된 산화성 간세포 손상에 대한 소시호탕(小柴胡湯)의 효과김태수, 마진열, 서상희, 오수영, 이지선, 조원경대한한방내과학회 · 대한한방내과학회지2011 · 제32권 제4호淸肝解酒湯이 인체간세포의 Glutathione 생성에 미치는 영향김영철, 우홍정, 윤여광, 이장훈대한한방내과학회 · 대한한방내과학회지2004 · 제25권 제1호

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