H1299 인체폐암세포주에서 황기와 사삼의 항암 시너지 효과
Synergistic Anticancer Effect of Astragalus Membranaceus and Adenophora Triphylla Var. Japonica in H1299 Human Lung Carcinoma Cells
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The anti-cancer effects of Astragalus membranaceus (AM) and Adenophora triphylla var. japonica (AT) have been described. Each of their effects mainly focused on the immunopotentiating and apoptosis inducing-ability in several cancer cell lines. Although the combination of AM and AT is occasionally used in Chinese medicine to treat lung cancers, their synergistic effect has not been proved yet. This study was designed to verify whether AM combined with AT exhibits a synergistic anti-cancer effect in H1299 human lung carcinoma cells. The ethanol extracts of AM (EAM) and AT (EAT) showed only slight cytotoxicity in H1299 cells when treated alone. However, the combination of EAM and EAT markedly suppressed the cell growth measured by MTT assay and trypan blue counting assay. In addition, co-treatment of EAM with EAT significantly reduced the colony-forming ability compared with single treatment of EAM or EAT in H1299 cells. We demonstrated that the synergistic effect of AM and AT was related with apoptosis induction proved by an accumulation of chromatin condensation, annexin V-positive cells, sub-G1 phase population, and cleaved-PARP expression, which were not observed by single treatment of EAM or EAT. In conclusion, the combination of EAM and EAT exhibited superior anti-cancer activity in H1299 cells than single treatment of EAM or EAT. We suggest that EAM combined with EAT might be a novel therapeutic option for lung cancer patients, and provide a reference for the development of more effective combination of Chinese herbs to treat lung cancer.
Abstract
The anti-cancer effects of Astragalus membranaceus (AM) and Adenophora triphylla var. japonica (AT) have been described. Each of their effects mainly focused on the immunopotentiating and apoptosis inducing-ability in several cancer cell lines. Although the combination of AM and AT is occasionally used in Chinese medicine to treat lung cancers, their synergistic effect has not been proved yet. This study was designed to verify whether AM combined with AT exhibits a synergistic anti-cancer effect in H1299 human lung carcinoma cells. The ethanol extracts of AM (EAM) and AT (EAT) showed only slight cytotoxicity in H1299 cells when treated alone. However, the combination of EAM and EAT markedly suppressed the cell growth measured by MTT assay and trypan blue counting assay. In addition, co-treatment of EAM with EAT significantly reduced the colony-forming ability compared with single treatment of EAM or EAT in H1299 cells. We demonstrated that the synergistic effect of AM and AT was related with apoptosis induction proved by an accumulation of chromatin condensation, annexin V-positive cells, sub-G1 phase population, and cleaved-PARP expression, which were not observed by single treatment of EAM or EAT. In conclusion, the combination of EAM and EAT exhibited superior anti-cancer activity in H1299 cells than single treatment of EAM or EAT. We suggest that EAM combined with EAT might be a novel therapeutic option for lung cancer patients, and provide a reference for the development of more effective combination of Chinese herbs to treat lung cancer.
참고문헌 (38)
- Siegel RL, "Cancer statistics, 2016", CA : A Cancer Journal for Clinicians, 66(1), pp.7~30 (2016)
- "National Cancer Information Center"[URL]
- Liu LK, "Exploraion of the Pathogenesis and Therapeutic Methods of Lung Cancer", China Journal of Basic Medicine In Traditional Chinese Medicine, 10(5), pp.75~ (2004)
- Geng G, "Efficacy of Yiqi Yangyin in the treatment of advanced non-small cell lung cancer", Nei Mongol Journal of Traditional Chinese Medicine, 4, pp.4~5 (1991)
- Ma CY, "Application of Yiqi Yangyin in lung cancer", Journal of Shandong college of traditional chinese medicine, 20(3), pp.178~179 (1996)
- Zhang T, "Clinical study on integrated Chinese and western medicine combined with radiotherapy in treatment of patients with advanced lung cancer", Journal of Clinical and Experimental Medicine, 12(2), pp.108~110 (2013)
- Liang F, "Effect of Yiqi Yangyin Jiedu Formula on T-Cell Subsets and Shifting of Th1", Th2 Balance in NSCLC Patients, 2011, pp.25(5), 8~10
- The Jointly Published Textbook Compilation Committee of Oriental College of Medicine Nationwide Department, "Herbology" (2004), Younglim press
- Xie ZF, "Classified dictionary of traditional Chinese medicine", pp.374~ (1994), New World Press
- Rios JL, "A review of the pharmacology and toxicology of Astragalus", Phytotheraphy Research, 11, pp.411~418 (1997)
- Huh J, "Donguibogam" (1981), Daeseong Culture & Cultural Company
- Hong MY, "Effects of Acupuncture and Radix Astragali aqua-acupuncture at Synsu(BL23)on transcriptional expression of mouse cytokine IL-1α", Journal of Pathology in Korean medicine, 13(2), pp.33~40 (1999)
- Bae WY, "The effect of Astragali Radix Aqua-acupuncture on immune responce induced by methotrexate in mice", The Journal of Korean Acupuncture & Moxibustion Medicine Society, 15(1), pp.49~66 (1994)
- Ryu JS, "Effect of Astragali Radix Aqua-acupuncture Solution on the Initiation Carcinogenesis", Korean Journal of Acupuncture, 18(2), pp.1~6 (2001)
- Duan P, "Clinical study on effect of Astragalus in efficacy enhancing and toxicity reducing of chemotherapy in patients of malignant tumor", Zhongguo Zhong Xi Yi Jie He Za Zhi, 22, pp.515~517 (2002)
- Zou YH, "Effect of Astragalus injection combined with chemotherapy on quality of life in patients with advanced non-small cell lung cancer", Zhongguo Zhong Xi Yi Jie He Za Zhi, 23, pp.733~735 (2003)
- Chu DT, "Immunotherapy with Chinese medicinal herbs I. Immune restoration of local xenogeneic graft-versus-host reaction in cancer patients by fractionated Astragalus membranaceus in vitro", J Clin Lab Immunol, 25, pp.119~123 (1988)
- Shao BM, "A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb", Biochem Biophys Res Commun, 320(4), pp.1103~1111 (2004)
- Li Q, "Inhibiting effect of Astragalus polysaccharides on the functions of CD4+CD25 highTreg cells in the tumor microenvironment of human hepatocellular carcinoma", Chin Med J, 125(5), pp.786~793 (2012)
- Shao P, "Regulation on maturation and function of dendritic cells by Astragalus mongholicus polysaccharides", Int Immunopharmacol, 6, pp.1161~1166 (2006)
- Auyeung KK, "Astragalus saponins modulate cell invasiveness and angiogenesis in human gastric adenocarcinoma cells", J Ethnopharmacol, 141(2), pp.635~641 (2012)
- Shin DS, "The Experimental Studies on the Immunomodulational Effects of Adenophorae Radix", The Korea Journal of Herbology, 15(1), pp.31~43 (2000)
- Song MS, "Experimental Study on the Suppression Effect of Asthma and Immune Response Improvement of Adenophorae Radix Herbal-Acupuncture", pp.2014
- 임난영, "沙蔘 메탄올 추출물의 멜라닌생성 억제효과", 동의생리병리학회지, 18(3), pp.747~753 (2004), 한의병리학회
- Min SH, "A comparative Study on the Anti-oxidant Effect of Adenophorae Radix, Codonopsis lanceolatae Radix and Glehniae Radix cum Rhizoma", pp.2006
- Yu X, "Effects of Codonopsis lanceolata total sanponin on apoptosis in HepG2 cells", J Jilin Univ(Medicine Edition), 6, pp.41~ (2011)
- Lee KW, "Beta-Dxylopyranosyl-(1→3)-beta-D-glucuronopyranosyl echinocystic acid isolated from the roots of Codonopsis lanceolata induces caspase-dependent apoptosis in human acute promyelocytic leukemia HL-60 cells", Biol Pharm Bull, 28, pp.854~859 (2005)
- Wang L, "Codonopsis lanceolata extract induces G0", G1 arrest and apoptosis in human colon tumor HT-29 cells involvement of ROS generation and polyamine depletion, 2011, pp.49, 149~154
- Byeon SE, "Regulatory effects of Codonopsis lanceolata on gene expression of GM-CSF in macrophage-like cells", J Ethnopharmacol, 123, pp.185~189 (2009)
- 소준노, "더덕추출물에 의한 bFGF-유도 시험관내 혈관신생의 억제", KSBB Journal, 18(1), pp.25~29 (2003), 한국생물공학회
- Borowicz S, "The soft agar colony formation assay", J Vis Exp, 27(92), pp.e51998~ (2014)
- van Engeland M, "Annexin V-affinity assay : a review on an apoptosis detection system based on phosphatidylserine exposure", Cytometry, 31(1), pp.1~9 (1998)
- Kischkel FC, "Cytotoxicity-dependent APO-1(Fas", CD95)-associated proteins form a death-inducing signaling complex(DISC)with the receptor, 1995, pp.14(22), 5579~5588
- Wang L, "Dissection of mechanisms of Chinese medicinal formula Realgar-Indigo naturalis as an effective treatment for promyelocytic leukemia", Proc Natl Acad Sci USA, 105(12), pp.4826~4831 (2008)
- Ma XH, "Synergistic therapeutic actions of herbal ingredients and their mechanisms from molecular interaction and network perspectives", Drug Discov Today, 14(11-12), pp.579~588 (2009)
- Kim HU, "A systems approach to traditional oriental medicine", Nat Biotechnol, 33(3), pp.264~268 (2015)
- 박선동, "當歸補血湯의 배합비율에 따른 대장암 세포주 HCT116의 세포사멸 효과", 대한본초학회지, 21(2), pp.37~46 (2006), 대한본초학회
- Son CK, "Composition consisted of Astragalus membranaceus and Salvia miltiorrhiza for reduction of side effects of anticancer drug, anti-metastasis and anti-fatigue", ~ (2014)