폐암세포에서 백렴의 항암효능연구 ― Bcl-2 family 단백조절을 통한 자가사멸―
The anti-cancer effects of Ampelopsisradix Extract (AE) on A549 cells ― The role of Bcl-2 family protein on the AE-induced apoptosis―
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Objective: The aim of this study is to evaluate anti-cancer effects of Ampelopsisradix Extract (AE) on human lung cancer A549 cells. Method: The apoptotic activities and cell growth arrest activities of AE were measured using 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The molecules involved in apoptotic process were assessed by western blotting. Result: Treatment of AE potently reduced cell viability in a dose-dependent manner in A549 cells. Bax and Bad levels were increased by AE with a concomitant decrease of Bcl-xL. In addition, AE at the low dose (30 ㎍/㎖) significantly inhibited cell growth in the presence of serum. Conclusion: AE has the potential as a therapeutic agent against lung cancer.
Abstract
Objective: The aim of this study is to evaluate anti-cancer effects of Ampelopsisradix Extract (AE) on human lung cancer A549 cells. Method: The apoptotic activities and cell growth arrest activities of AE were measured using 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The molecules involved in apoptotic process were assessed by western blotting. Result: Treatment of AE potently reduced cell viability in a dose-dependent manner in A549 cells. Bax and Bad levels were increased by AE with a concomitant decrease of Bcl-xL. In addition, AE at the low dose (30 ㎍/㎖) significantly inhibited cell growth in the presence of serum. Conclusion: AE has the potential as a therapeutic agent against lung cancer.
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