하고초 추출물의 streptozotocin 유발 당뇨 랫트 사구체 손상 개선 효과
Protective Effects of Prunella Vulgaris on Glomerular Injury in Streptozotocin-Induced Diabetic Rats
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Prunella vulgaris, well-known traditional medicinal plant, is used for the cure of abscess, scrofula, hypertension and urinary diseases. Diabetic nephropathy is the most common cause of end-stage renal disease. The pathological characteristics of diabetic nephropathy are glomerular and tubular basement membrane thickening. The aim of the present study was to evaluate the effect of Prunella vulgaris, on diabetic glomerular injury in streptozotocin -induced diabetes rats. Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ; 45 mg/kg) and confirmed by random glucose level higher than ≤ 300 mg/dL. The experimental rats were divided into five groups: control group (Male SD rats), STZ group (Male SD rats injected STZ), Aminoguanidine group (Male SD rats injected STZ + AG 100 mg/kg/day), Low dose group (Male SD rats injected STZ + APV 100 mg/kg/day), High dose group (Male SD rats injected STZ + APV 300 mg/kg/day). AG or APVs were administered once a day for 8 weeks. Body weight and food/water intake were measured every four weeks. At the end of study, the kidneys were collected and cut into pieces for immunohistochemistry and western blot analysis. Our study showed that body weight and water/food intake were no significant differences between untreated STZ-induced diabetic rat and APV treated-STZ rat. However, phosphorylation of receptor-regulated Smads (Smad3) was significantly decreased in APV treated-STZ rat as compared with the diabetic group. In addition, APV was improved nephrin level in kidney tissue. Therefore, we suggest that APV has a protective effect against STZ-induced diabetic glomerular injury.
Abstract
Prunella vulgaris, well-known traditional medicinal plant, is used for the cure of abscess, scrofula, hypertension and urinary diseases. Diabetic nephropathy is the most common cause of end-stage renal disease. The pathological characteristics of diabetic nephropathy are glomerular and tubular basement membrane thickening. The aim of the present study was to evaluate the effect of Prunella vulgaris, on diabetic glomerular injury in streptozotocin -induced diabetes rats. Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ; 45 mg/kg) and confirmed by random glucose level higher than ≤ 300 mg/dL. The experimental rats were divided into five groups: control group (Male SD rats), STZ group (Male SD rats injected STZ), Aminoguanidine group (Male SD rats injected STZ + AG 100 mg/kg/day), Low dose group (Male SD rats injected STZ + APV 100 mg/kg/day), High dose group (Male SD rats injected STZ + APV 300 mg/kg/day). AG or APVs were administered once a day for 8 weeks. Body weight and food/water intake were measured every four weeks. At the end of study, the kidneys were collected and cut into pieces for immunohistochemistry and western blot analysis. Our study showed that body weight and water/food intake were no significant differences between untreated STZ-induced diabetic rat and APV treated-STZ rat. However, phosphorylation of receptor-regulated Smads (Smad3) was significantly decreased in APV treated-STZ rat as compared with the diabetic group. In addition, APV was improved nephrin level in kidney tissue. Therefore, we suggest that APV has a protective effect against STZ-induced diabetic glomerular injury.
참고문헌 (26)
- 이은영, "당뇨병성 신증 연구의 최신지견", 대한내과학회지, 75(6), pp.646~650 (2008), 대한내과학회
- Eddy AA, "Expression of genes that promote renal interstitial fibrosis in rats with proteinuria", Kidney Int, 49, pp.S49~S54 (1996)
- Bharali R, "Signal transduction by the TGF-beta superfamily", Science, 296, pp.1646~1647 (2002)
- Leask A, "TGF-beta signaling and the fibrotic response", FASEB J, 18, pp.816~827 (2004)
- 박수현, "모링가 뿌리 추출물에 대한 신장섬유화 억제 효과", 생명과학회지, 22(10), pp.1371~1377 (2012), 한국생명과학회[DOI]
- Cho HJ, "Suppression of PAI-1 expression through inhibition of the EGFR-mediated signaling cascade in rat kidney fibroblast by ascofuranone", J Cell Biochem, 107, pp.335~344 (2009)
- Shibuya K, "N-Acetyl-seryl-aspartyl-lysyl-proline prevents renal insuffi ciency and mesangial matrix expansion in diabetic db", db mice, 2005, pp.54, 838~845
- Ruotsalainen V, "Nsphrin is specifically located at the slit diaphragm of glomerular podocytes", Proc Natl Acad Sci USA, 96, pp.7962~7967 (1999)
- 김병권, "신 사구체 질환에서 Nephrin, Glomerular Epithelial Cell Protein-1 (GLEPP1) 및 WT-1 발현에 관한 연구", Journal of Pathology and Translational Medicine, 36(1), pp.21~29 (2002), 대한병리학회
- 장현주, "하고초물추출물이 LPS로 활성화된 Raw 264.7 cell에서의 염증매개물질 억제효과", 동의생리병리학회지, 23(3), pp.599~607 (2009), 한의병리학회
- Ryu SY, "Anti-allergic and anti-inflammatory triterpenes from the herb of Prunella vulgaris", Planta Med, 66, pp.358~360 (2000)
- 본초학 교수, "본초학", pp.169~ (1992), 영림사
- Wang ZJ, "Studies on the chemical constituents from prunella vulgaris", Acta Pharm Sin, 34, pp.679~681 (1999)
- 이수정, "하고초 메탄올 추출물의 항산화 활성", 한국식품영양과학회지, 37(12), pp.1535~1541 (2008), 한국식품영양과학회
- Kim JS, "Quantitative determination of ursolic acid from Prunellae herba", Kor J Pharmacogn, 31, pp.416~420 (2001)
- Lam TL, "A comparison of human immunodeficiedcy virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs", Life Sci, 67, pp.2889~2896 (2000)
- Soedamah-Muthu SS, "Soluble vascular cell adhesion molecule-1 and soluble E-selectin are associatedwith micro-and macrovascular complications in Type 1 diabetic patients", J Diabetes Complicat, 20, pp.188~195 (2006)
- Guo B, "MAPK", AP-1-dependent regulation of PAI-1 gene expression by TGF-beta in rat mesnagial cells, 2005, pp.68, 972~984
- Attisano L, "Signal transduction by the TGF-beta superfamily", Sceince, 296, pp.1646~1647 (2002)
- Shi Y, "A structural basis for mutational inactivation of the tumour suppressor Smad4", Nature, 388, pp.87~93 (1997)
- Zhao TT, "Chaihuang-Yishen granule inhibits diabetic kidney disease in rats through blocking TGF-β", Smad3 signaling, 2014, pp.9(3), e90807~
- Yoon JJ, "Protective role of oryeongsan against renal inflammation and glomerulosclerosis in db", db mice, 2014, pp.42(6), 1431~1452
- Wang A, "Interference with TGF-beta signaling by Smad3-knockout in mice limits diabetic glomerulosclerosis without affecting albuminuria", Am J Physiol Renal Physiol, 293, pp.F1657~F1665 (2007)
- 이은영, "당뇨병성 신증에서 angiopoietins 유전자의 변화", Kidney Research and Clinical Practice, 26(3), pp.311~319 (2007), 대한신장학회
- Putaala H, "The murine nephrin gene is specifically expressed in kidney, brain and pancreas : inactivation of the gene leads to massive proteinuria and neonatal death", Hum Mol Genet, 10, pp.1~8 (2001)
- Hwang SM, "Anti-Proliferative Effect of an Aqueous Extract of Prunella vulgaris in Vascular Smooth Muscle Cells", Evid Based Complement Alternat Med, pp.936463~ (2013)