加味五苓散이 Streptozotocin으로 유발된 흰쥐의 糖尿病性 腎症에 미치는 영향
The Effects of Gamioryung-san Extracts on Streptozotocin-induced Diabetic Nephropathy Rats
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Objectives : The object of this study was to observe the effects of Gamioryung-san (GOS), which consists of 22 types of herbs, on streptozotocin (STZ)-induced diabetic nephropathy rats. Methods : Three different dosages of GOS were orally administered once a day for 28 days from 3 weeks after STZ treatment. Six groups, each of 8 rats per group were used. Changes on the body weights, blood glucose levels, serum BUN and creatinine levels, urine volumes, and UAER were observed with changes on the kidney malondialdehyde contents and glutathione, dismutase and catalase contents. In addition, histopathology of kidney, pancreas, thymus and spleen were observed. The results were compared with antioxidant silymarin 100 mg/kg, of which the effects on STZ -induced diabetes and related complications are already confirmed. Results : As a result of treatment of GOS 800, 400 or 200 mg/kg for 28 days, STZ-induced decreases of body weights, hyperglycemia, atrophic changes of pancreatic islets with decreases of insulin-immunoreactive cells and decreases of glucagon -immunoreactive cells were inhibited dose-dependently. Increases in kidney weight, serum BUN and creatinine levels, urine volumes, UAER, vasodilated atrophic glomerulus and abnormal tubules were inhibited dose-dependently. Also increases of kidney MDA contents and decreases of GSH contents, SOD and CAT activities, decreases of thymus and spleen weights, and atrophic changes at histopathological observation were also inhibited. The effects of GOS 400 mg/kg showed similar effects to silymarin 100 mg/kg. Conclusions : These results suggest that 400 mg/kg of GOS retarded the STZ-induced diabetic nephropathies as similarly to silymarin 100 mg/kg, through modulations of oxidative stress and immune systems.
Abstract
Objectives : The object of this study was to observe the effects of Gamioryung-san (GOS), which consists of 22 types of herbs, on streptozotocin (STZ)-induced diabetic nephropathy rats. Methods : Three different dosages of GOS were orally administered once a day for 28 days from 3 weeks after STZ treatment. Six groups, each of 8 rats per group were used. Changes on the body weights, blood glucose levels, serum BUN and creatinine levels, urine volumes, and UAER were observed with changes on the kidney malondialdehyde contents and glutathione, dismutase and catalase contents. In addition, histopathology of kidney, pancreas, thymus and spleen were observed. The results were compared with antioxidant silymarin 100 mg/kg, of which the effects on STZ -induced diabetes and related complications are already confirmed. Results : As a result of treatment of GOS 800, 400 or 200 mg/kg for 28 days, STZ-induced decreases of body weights, hyperglycemia, atrophic changes of pancreatic islets with decreases of insulin-immunoreactive cells and decreases of glucagon -immunoreactive cells were inhibited dose-dependently. Increases in kidney weight, serum BUN and creatinine levels, urine volumes, UAER, vasodilated atrophic glomerulus and abnormal tubules were inhibited dose-dependently. Also increases of kidney MDA contents and decreases of GSH contents, SOD and CAT activities, decreases of thymus and spleen weights, and atrophic changes at histopathological observation were also inhibited. The effects of GOS 400 mg/kg showed similar effects to silymarin 100 mg/kg. Conclusions : These results suggest that 400 mg/kg of GOS retarded the STZ-induced diabetic nephropathies as similarly to silymarin 100 mg/kg, through modulations of oxidative stress and immune systems.
참고문헌 (34)
- 박정탁, "당뇨병성 신증의 병태생리", 대한내과학회지, 77(6), pp.670~677 (2009), 대한내과학회
- 전국 한의과대학 신계내과학교실, "신계내과학", pp.95~ (2011), 군자출판사
- Fujii H, "Oxidative and nitrosative stress and progression of diabetic nephropathy in type 2 diabetes", Am J Nephrol, 31, pp.342~345 (2010)
- Briede J, "Effect of cerebrocrast on body and organ weight, food and water intake, and urine output of normal rats", Cell Biochem Funct, 26, pp.908~915 (2008)
- Ceriello A, "New insights on non-enzymatic glycosylation may lead to therapeutic approaches for the prevention of diabetic complications", Diabet Med, 9, pp.297~299 (1992)
- Bolling BW, "Tea and health: preventive and therapeutic usefulness in the elderly", Curr Opin Clin Nutr Metab Care, 12, pp.42~48 (2009)
- Vessal G, "Silymarin and milk thistle extract may prevent the progression of diabetic nephropathy in streptozotocin-induced diabetic rats", Ren Fail, 32, pp.733~739 (2010)
- Kavutcu M, "Reduced enzymatic antioxidant defense mechanism in kidney tissues from gentamicin-treated guinea pigs: effects of vitamins E and C", Nephron, 72, pp.269~274 (1996)
- Lowry OH, "Protein measurement with the Folin phenol reagent", J Biol Chem, 193, pp.265~275 (1951)
- Draper HH, "Methods in Enzymology", pp.421~ (1990), Academic Press
- Eyer P, "Evaluation of the micromethod for determination of glutathione using enzymatic cycling and Ellman's reagent", Anal Biochem, 153, pp.57~56 (1986)
- Beauchamp C, "Superoxide dismutase: improved assays and an assay applicable to acrylamide gels", Anal Biochem, 44, pp.276~278 (1971)
- Sternberger LA, "The unlabeled antibody peroxidase -antiperoxidase (PAP) method, In Immunocytochemistry", pp.104~106 (1979), John Wiley & Sons
- Kang SM, "Ameliorative anti-diabetic activity of Dangnyosoko, a Chinese herbal medicine, in diabetic rats", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 71(6), pp.1527-1534~ (2007), JAPAN SOC BIOSCI BIOTECHN AGROCHEM
- 장태수, "증상을 중심으로 본 消渴과 糖尿病의 관계", 대한한방내과학회지, 2, pp.61~69 (1985)
- 杜鎬京, "東醫腎系學", pp.514~517 (2003), 성보사
- Osicka TM, "Prevention of albuminuria by aminoguanidine or ramipril in streptozotocin-induced diabetic rats is associated with the normalization of glomerular protein kinase C", Diabetes, 49, pp.87~89 (2000)
- Giugliano D, "Oxidative stress and diabetic vascular complications", Diabetes Care, 19, pp.257~256 (1996)
- Quine SD, "Effects of (-)-epicatechin, a flavonoid on lipid peroxidation and antioxidants in streptozotocin-induced diabetic liver, kidney and heart", Pharmacol Rep, 57, pp.610~615 (2005)
- Anjaneyulu M, "Diltiazem attenuates oxidative stress in diabetic rats", Ren Fail, 27, pp.335~334 (2005)
- Wellington K, "Silymarin: a review of its clinical properties in the management of hepatic disorders", Bio Drugs, 15, pp.465~489 (2001)
- Huseini HF, "The efficacy of Silybum marianum (L.) Gaertn. (silymarin) in the treatment of type II diabetes: a randomized, double-blind, placebo-controlled, clinical trial", Phytother Res, 20, pp.1036~1039 (2006)
- 김영석, "단삼이 당뇨병성 신병증 Rat의 신기능 및 조직학적 변화에 미치는 영향", 대한한방내과학회지, 29(3), pp.787~799 (2008), 대한한방내과학회
- 홍광해, "石膏가 streptozotocin으로 유발된 rat의 糖尿病性 腎症에 미치는 影響", 대한한방내과학회지, 29(4), pp.1025~1036 (2008), 대한한방내과학회
- 서수환, "水蛭이 Streptozotocin으로 유발된 Rat의 糖尿病性 腎症에 미치는 影響", 대한한의학회지, 29(4), pp.133~145 (2008), 대한한의학회
- 조수인, "薏苡仁 추출물의 경구투여가 Streptozotocin에 의한 흰쥐의 당뇨병성 신증에 미치는 영향", 대한본초학회지, 21(3), pp.75~81 (2006), 대한본초학회
- 윤관희, "蔘", b>地, b>淫羊, pp.b>이 Streptozotocin으로 유발된 흰쥐의 糖尿病性 腎症에 미치는 영향, 대한한의학회지[DOI]
- 조현주, "蔘耆地黃湯加丹蔘,大黃,紅花가 Streptozotocin으로 유발된 생쥐의 糖尿病性 腎症에 미치는 영향", 대한한방내과학회지, 26(4), pp.767~775 (2005), 대한한방내과학회
- 신현권, "韓藥複合處方이 Streptozotocin으로 유발된 糖尿病 흰쥐의 血糖 및 糖尿病性 腎證 발현에 미치는 영향", 대한침구의학회지, 25(3), pp.110~120 (2008), 대한침구의학회
- 나건호, "糖尿1號方의 藥鍼 및 口腔投與가 Streptozotocin에 의한 흰쥐의 糖尿病性 腎症에 미치는 영향", 대한침구의학회지, 22(4), pp.1~12 (2005), 대한침구의학회
- 한방약리학교재편찬위원회, "한방약리학", pp.126~ (2010), 신일상사
- Pinzone Fox, ML, "Plasma leptin concentration increases early during highly active antiretroviral therapy for acquired immunodeficiency syndrome, independent of body weight", J Endocrinol Invest, 28, pp.1~3 (2005)
- Montilla P, "Red wine prevents brain oxidative stress and nephropathy in streptozotocin-induced diabetic rats", J Biochem Mol Biol, 38, pp.539~544 (2005)
- Sodikoff CH, "Laboratory profiles of small animal diseases. A guide to laboratory diagnosis", pp.1~36 (1995), Mosby