산화적 스트레스에 대한 천마 추출물의 신경세포 보호 및 항산화 효과
Neuroprotective and Anti-oxidant Effects of Gastrodiae Rhizoma Extracts against Hydrogen Peroxide-induced Cytotoxicity in SH-SY5Y Cells
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
We recently reported that Gastrodia elata extracts (GEE) had an effects to protect against lipopolysaccharide-induced cognitive impairment in vivo model. In this study, we investigated the neuroprotective effects and the mechanism of action of GEE in hydrogen peroxide (H2O2)-induced cell death of SH-SY5Y human neuroblastoma cell. The SH-SY5Y cells were divided into five groups, including control(non-treated group), 100 μM H2O2, 100, 200, 500 ㎍/㎖ GEE+ 100 μM H2O2 groups. Pre- and co-treatment with GEE prevented cell death induced by 100 μM H2O2 for 24 h in SH-SY5Y cells. Our findings also showed that anti-oxidants enzymes (Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase) were up-regulated by 100 μM H2O2. But GEE suppressed H2O2-induced anti-oxidants enzymes decrease in a dose-dependent manner. Treatment with GEE also inhibited phosphorylation of eukaryotic initiation factor-2α (eIF-2α) and p38 by H2O2. Taken together, the neuroprotective effects of GEE in terms of recovery of antioxidant enzymes expression, down-regulation of eIF-2α and p38 phosphorylation, and inhibition of cell death are associated with reduced oxidative stress in SH-SY5Y cells.
Abstract
We recently reported that Gastrodia elata extracts (GEE) had an effects to protect against lipopolysaccharide-induced cognitive impairment in vivo model. In this study, we investigated the neuroprotective effects and the mechanism of action of GEE in hydrogen peroxide (H2O2)-induced cell death of SH-SY5Y human neuroblastoma cell. The SH-SY5Y cells were divided into five groups, including control(non-treated group), 100 μM H2O2, 100, 200, 500 ㎍/㎖ GEE+ 100 μM H2O2 groups. Pre- and co-treatment with GEE prevented cell death induced by 100 μM H2O2 for 24 h in SH-SY5Y cells. Our findings also showed that anti-oxidants enzymes (Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase) were up-regulated by 100 μM H2O2. But GEE suppressed H2O2-induced anti-oxidants enzymes decrease in a dose-dependent manner. Treatment with GEE also inhibited phosphorylation of eukaryotic initiation factor-2α (eIF-2α) and p38 by H2O2. Taken together, the neuroprotective effects of GEE in terms of recovery of antioxidant enzymes expression, down-regulation of eIF-2α and p38 phosphorylation, and inhibition of cell death are associated with reduced oxidative stress in SH-SY5Y cells.
참고문헌 (22)
- Current Status and Implications of disease studies related to aging, "Kim HB", Korea Institute of Science & Technology Evaluation and Planning, ~ (2012)
- Budzynska B, "Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice", Psychopharmacology, 232(5), pp.931~942 (2015)
- Murakami S, "Neuroprotective effects of metallothionein against rotenone-induced myenteric neurodegeneration in parkinsonian mice", Neurotox Res, 26(3), pp.285~298 (2014)
- Stadtman ER, "Reactive oxygen-mediated protein oxidation in aging and disease", Chem Res Toxicol, 10(5), pp.485~494 (1997)
- 김수현, "인간 HepG2 세포에서 더덕 및 도라지 에틸아세테이트 분획물의 항산화 효과에 의한 세포보호 효과", 한국식품과학회지, 40(6), pp.696~701 (2008), 한국식품과학회
- 유소현, "상백피 에탄올 추출물의 항산화 활성 및 H2O2로 유도한 PC12 신경세포 보호 효과", 한국식품영양과학회지, 47(5), pp.519~527 (2018), 한국식품영양과학회
- 김꽃별, "산화적 스트레스에 대한 여주 (Momordica charantia) 추출물의 항산화 효과 및 세포사멸 억제 기전을 통한 신경세포보호효과", Journal of Nutrition and Health, 50(5), pp.415~425 (2017), 한국영양학회
- 이숭인, "비자 열수 추출물의 항산화 활성 및 뇌신경세포 보호효과 연구", 대한본초학회지, 32(6), pp.41~48 (2017), 대한본초학회[DOI]
- Taguchi H, "Studies on the constituents of Gastrodia elata BLUME", Chem Pharm Bull, 29, pp.55~62 (1981)
- 정영수, "癡呆病態모델에서 天麻의 神經細胞 損傷 保護效果", 동의신경정신과학회지, 21(2), pp.125~140 (2010), 대한한방신경정신과학회
- 윤유석, "천마가 중대뇌동맥 폐쇄 흰쥐의 신경세포 자연사에 미치는 영향", 한방재활의학과학회지, 19(3), pp.1~13 (2009), 한방재활의학과학회
- 김우철, "천마를 이용한 기억력 향상 효과 연구", 동의신경정신과학회지, 24(1), pp.27~43 (2013), 대한한방신경정신과학회
- 김하나, "천마의 용량에 따른 기억력 향상 효과에 대한 연구", 동의신경정신과학회지, 25(3), pp.243~252 (2014), 대한한방신경정신과학회
- Alzheimer’s Association, "2012 Alzheimer’s disease facts and figures", Alzheimer’s & Dementia, 8(2), pp.131~168 (2012)
- 권강범, "LPS에 의해 유도된 인지기능 손상모델에 대한 천마 추출물의 방어효과", 동의신경정신과학회지, 33(3), pp.277~285 (2022), 대한한방신경정신과학회[DOI]
- Anantharaman M, "Beta-amyloid mediated nitration of manganese superoxide dismutase : implication for oxidative stress in a APPNLH", NLH X PS-1P264L, Am J Pathol, pp.2006, 168[DOI]
- An H, "Oxidative damage induced by nano-titanium dioxide in rats and mice : A systematic review and meta-analysis", Biol Trace Elem Res, 194(1), pp.184~202 (2020)
- 김선환, "CoCl2 처리로 유도된 hypoxia상태에서 세포자살과 ER stress에 관련된 인자의 발현", 생명과학회지, 20(12), pp.1820~1828 (2010), 한국생명과학회
- Henshall DC, "Apoptosis signalling pathways in seizure-induced neuronal death and epilepsy", Biochem Soc Trans, (35), pp.421~423 (2007)
- Liu Z, "Protein kinase R-like ER kinase and its role in endoplasmic reticulum stress-decided cell fate", Cell Death Dis, (6), pp.e1822~ (2015)
- Chung MJ, "Neuroprotective effects of phytosterols and flavonoids from Cirsium setidens and Aster scaber in human brain neuroblastoma SK-N-SH cells", Life Sci, 148, pp.173~182 (2016)
- Pan J, "Reactive oxygen species-activated Akt", ASK1, Chem Res Toxicol, pp.2010, 23(3)[DOI]