MC903으로 유도하는 아토피피부염 생쥐 동물 모델 최적화: 유발 기간 단축 및 부작용 감소를 중심으로
Optimization of MC903-induced Atopic Dermatitis Mouse Model : Focusing on Reducing the Induction Period and Adverse Effects
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study was designed to establish an atopic dermatitis (AD) model using MC903 and was conducted to find out the optimal challenge concentration that can cause dermatitis symptoms enough to be experimentally verified while reducing adverse effects such as weight loss. MC903 was treated at concentrations of 2, 3, and 4 nmol/day, and evaluation of skin surface symptoms, water contents, histopathological changes, body weight changes, and spleen/body weight ratio was conducted. In addition, the expression level of thymic stromal lymphopoietin (TSLP) was also observed. In our results, MC903 induced AD skin lesions such as erythema, scab and fissure and lowered skin moisture level significantly. In addition, water holding capacities in the 3 or 4 nmol groups were diminished significantly compared to that in the control group. On the other hand, 4 nmol treatment induced a weight loss of up to 20% compared to control group. Finally, a higher level of TSLP expression was observed in the 3 nmol group than in the 2 nmol group or the 4 nmol group. Taken together, we propose a total 14-day protocol consisting of 3 days of sensitization (2 nmol/day) and 6 days of challenge (3 nmol/day).
Abstract
This study was designed to establish an atopic dermatitis (AD) model using MC903 and was conducted to find out the optimal challenge concentration that can cause dermatitis symptoms enough to be experimentally verified while reducing adverse effects such as weight loss. MC903 was treated at concentrations of 2, 3, and 4 nmol/day, and evaluation of skin surface symptoms, water contents, histopathological changes, body weight changes, and spleen/body weight ratio was conducted. In addition, the expression level of thymic stromal lymphopoietin (TSLP) was also observed. In our results, MC903 induced AD skin lesions such as erythema, scab and fissure and lowered skin moisture level significantly. In addition, water holding capacities in the 3 or 4 nmol groups were diminished significantly compared to that in the control group. On the other hand, 4 nmol treatment induced a weight loss of up to 20% compared to control group. Finally, a higher level of TSLP expression was observed in the 3 nmol group than in the 2 nmol group or the 4 nmol group. Taken together, we propose a total 14-day protocol consisting of 3 days of sensitization (2 nmol/day) and 6 days of challenge (3 nmol/day).
참고문헌 (18)
- 김은희, "아토피 피부염의 주증상에 대한 문헌적 고찰", 대한한의학원전학회지, 22(1), pp.207~228 (2009), 대한한의학원전학회
- Avena-Woods C, "Overview of atopic dermatitis", The American journal of managed care, 23(8 Suppl), pp.S115~S123 (2017)
- 김민지, "아토피피부염의 표현형과 내재형", Allergy Asthma & Respiratory Disease, 8(1), pp.9~14 (2020), 대한 소아알레르기 호흡기학회
- Indra AK, "Epidermal TSLP: a trigger factor for pathogenesis of atopic dermatitis", Expert review of proteomics, 10(4), pp.309~311 (2013)
- He R, "Thymic stromal lymphopoietin", Annals of the New York Academy of Sciences, 1183(1), pp.13~24 (2010)
- Li M, "Topical vitamin D3 and low-calcemic analogs induce thymic stromal lymphopoietin in mouse keratinocytes and trigger an atopic dermatitis", Proceedings of the National Academy of Sciences, 103(31), pp.11736~11741 (2006)
- Moosbrugger-Martinz V, "A mouse model for atopic dermatitis using topical application of vitamin D3 or of its analog MC903", Inflammation: Methods and Protocols, pp.91~106 (2017)
- Amano H, "Psychological stress can trigger atopic dermatitis in NC", Nga mice: an inhibitory effect of corticotropin-releasing factor, 2008, pp.33(3), 566~573
- 김형우, "배의 씨방 및 과피의 알콜 발효 추출물이 피부에 미치는 영향", 대한본초학회지, 24(1), pp.133~139 (2009), 대한본초학회
- Lyu JH, "Dangkwisoo-san, an herbal medicinal formula, ameliorates acute lung inflammation via activation of Nrf2 and suppression of NF-κB", Journal of ethnopharmacology, 140(1), pp.107~116 (2012)
- Jang S, "The ethanol extract of Caragana sinica ameliorated skin lesions in mice with contact dermatitis", Pharmacognosy Magazine, 18(77), pp.201~ (2022)
- Addor FA, "Atopic dermatitis: correlation between non‐damaged skin barrier function and disease activity", International journal of dermatology, 51(6), pp.672~676 (2012)
- Knor T, "Stratum corneum hydration and skin surface pH in patients with atopic dermatitis", Acta dermatovenerologica Croatica : ADC, 19(4), pp.242~247 (2011)
- Oghazian MB, "Vitamin D Intoxication with hypercalcemia due to overuse of supplement", Journal of Pharmaceutical Care, pp.114~116 (2013)
- Kabata H, "Targeted deletion of the TSLP receptor reveals cellular mechanisms that promote type 2 airway inflammation", Mucosal immunology, 13(4), pp.626~636 (2020)
- Imokawa G, "Decreased level of ceramides in stratum corneum of atopic dermatitis: an etiologic factor in atopic dry skin?", Journal of Investigative Dermatology, 96(4), pp.523~526 (1991)
- Simpson EL, "Emollient enhancement of the skin barrier from birth offers effective atopic dermatitis prevention", Journal of Allergy and Clinical Immunology, 134(4), pp.818~823 (2014)
- Kim MR, "A study on the distribution of staphylococcus aureus in atopic dermatitis", Journal of the Society of Cosmetic Scientists of Korea, 32(2), pp.93~97 (2006)