대장 선종 및 대장 선암에서 TGF-β1, 제2형 TGF-β 수용체 및 VEGF 단백의 면역조직화학적 발현
Expression of TGF-β1, TGF-β Receptor Type II and VEGF in Colorectal Adenomas and Adenocarcinomas
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목적: 내시경 또는 외과수술로 절제된 대장 선종과 대장 선암 조직을 대상으로 면역조직화학 염색을 시행하여 TGF-β1, TGF-βRII 및 VEGF의 발현정도를 비교 관찰함으로써 TGF-β1, TGF-βRII 및 VEGF이 대장 선암의 발생 과정 및 진행에 관련이 있는지를 알아보고자 하였다. 대상 및 방법: 대장 선종 조직 20예와 대장 선암 40예를 대상으로 tissue microarray를 제작하여 TGF-β1, TGF-βRII 및 VEGF에 대한 면역조직화학 염색을 시행하였다. 결과: 대장 선종군에서 TGF-β1, TGF-βRII 및 VEGF의 염색강도는 고이형성군에서 증가하였으며 TGFβRII의 염색강도는 고이형성군에서 유의하게 높았다(p=0.021). 대장 선암군에서 TGF-β1, TGF-βRII 및 VEGF의 발현 및 염색강도는 선종군과 비교하여 유의하게 높았으나(p＜0.001), 염색강도와 나이, 성별, 종괴의 크기, 병기 및 분화도 사이에 유의한 연관성은 없었다. 결론: 대장 선암에서 TGF-β1, TGFβRII 및 VEGF의 염색강도의 증가는 이들 단백이 대장 선암의 형성과정 및 진행에 중요한 역할을 한다는 것을 시사한다. TGF-β의 세포 성장 억제기능의 소실은 대장암의 발암과정 중 비교적 초기에 일어나는 것으로 생각한다.
Abstract
Background/Aims: The aim of study was to investigate the expression of TGF-β, TGF-RII and VEGF determined by immunohistochemical analysis with a comparison of the clinicopathological parameters such as tumor size, grade of dysplasia, lymph node metastasis and Dukes' stage in colorectal adenomas and adenocarcinomas, by use of a tissue microarray method. Methods: The expression of TGF-β1, TGF-βRII, and VEGF was determined by immunohistochemistry in 20 adenomas and 40 adenocarcinomas. Tissue microarrays consisting of 2 mm cores from corresponding blocks were constructed and stained. Results: In adenomas, the staining intensity of TGF-β, TGF-βRII and VEGF was increased in a high-grade dysplasia group of patients as compared a with low-grade dysplasia group of patients, respectively. The staining intensity of TGF-βRII was significantly increased in a high-grade dysplasia group of patients than a low-grade dysplasia group of patients (p =0.021). For the adenocarcinomas, the expression and staining intensity of TGF-β1, TGF-βRII and VEGF were increased as compared with the adenomas (p＜0.001). However, no significant correlation was observed between the staining intensity of TGF-β, TGF-βRII and VEGF and the clinicopathological parameters. Conclusions: The increased expression of TGF-β1, TGF-βRII and VEGF in colorectal adenocarcinoma suggests a role for these proteins in colorectal carcinogenesis. Loss of the growth-inhibitory effect of TGF-β may commence in the early stage of colorectal carcinogenesis. (Korean J Gastrointest Endosc 2007;35:313-320)
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