뇌졸중을 일으킨 실험쥐에서 Colchicine과 Chloroquine 혼합 투여의 효과

Abstract

Background : It is well known that microglia/macrophage infiltrate in the ischemic brain. Since activated microglia/macrophages may produce substances toxic to dying neurons, agents inhibiting the function of these cells may alleviate the ischemic brain injury. Methods : We attempted to administer colchicine plus chloroquine, alleged microglia/macrophage inhibitors, in the rats subjected to permanent(n=20) and transient(n=20) middle cerebral artery(MCA) occlusion. The animals were sacrificed at 96 hours after the MCA occlusion, and the size of the brain infarcts was measured. Immunoreactivity of astrocytes and macrophages was also assessed. Results : In permanent ischemia model, the rats treated with colchicine plus chloroquine tended to have small sized infarcts as compared to the vehicle treated rats, which, however, was not statistically significant(p=0.089). In transient ischemia model, there was no significant difference in the size of the ischemic lesions between the two groups. The immunoreactivity of monocyte/macrohage appeared less dense, and the morphology of these cells was round in the treated animals as compared to the control group. Conclusions : Our results show that colchicine plus choloroquine treatment may reduce the function of monocyte/macrophages. However, the treatment did not significantly reduce the size of the infarct in our animal model. Further studies with different strategies for monocyte/macrophage suppression are warranted in the permanent ischemia model. Korean Journal of Stroke 2000;2(1): 86~90