본문 바로가기
KERD
논문KCI 등재

Toxoplasma gondi Inhibits Apoptosis in Infected Cells by Caspase Inactivation and NF-κB Activation

김지영|안명희|민득영|전혜선|정재원|류재숙
연세대학교의과대학 (Yonsei University College of Medicine)|2006.12|Yonsei Medical Journal|제47권 제6호|pp. 862-869

2

조회

0

다운로드

3

인용

0

참고문헌

주제분류의학일반

초록

Abstract

Our experiments aimed to clarify the mechanism by which host cell apoptosis is inhibited by infection with the intracellular protozoan parasite, Toxoplasma gondii (T. gondii). Mouse spleen cells were cultured in 6-well plates with RPMI 1640/ 10% FBS at 37℃, in a 5% CO2 atmosphere. Apoptosis of spleen cells was induced by actinomycin-D (AD) treatment for 1 h prior to infection with T. gondii. A variety of assays were used to assess the progression of apoptosis: DNA size analysis on agarose gel electrophoresis, flow cytometry with annexin V/PI staining, and analysis of expression levels of Bcl-2 family and NF- B mRNA and proteins by RT-PCR, Western blotting, and EMSA. Additionally, transmission electron microscopy (TEM) was performed to observe changes in cell morphology. Fragmentation of DNA was inhibited in spleen cells treated with AD and T. gondii 5 h and 18 h post infection, respectively, and flow cytometry studies showed a decreased apoptotic rates in AD and T. gondii treated spleen cells. We observed decreased expression of Bax mRNA and protein, while levels of Bcl-2 mRNA remained constant in spleen cells treated with AD and T. gondii. Caspase 3 and PARP were inactivated in cells treated with AD and T. gondii, and increased levels of cleaved caspase 8 were also observed. Analysis of EMSA and Western blot data suggests that activation of transcription factor NF- B may be involved in the blockade of apoptosis by T. gondii. TEM analysis showed nuclear fragmentation and chromatin condensation occurring in spleen cells treated with AD; however, such apoptosis- associated morphological changes were not observed in cells treated with both AD and T. gondii tachyzoites. Together, these data show that T. gondii infection inhibits AD induced apoptosis via caspase inactivation and NF- B activation in mouse spleen cells.

참고문헌 (22)

  1. "Inhibition of caspase activation and requirement for NF- B function in the Toxoplasma gondii-mediated blockade of host apoptosis", 116, pp.4345~4558 (2003)
  2. "Apoptosis of primary-culture rat microglial cells induced by pathogenic Acanthamoeba spp", 7, pp.510~514 (2000)
  3. "Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba histolytica", 42, pp.205~208 (2004)
  4. "NADPH oxidase-derived reactive oxygen species- mediated activation of ERK1", 2 is required for apoptosis of human neutrophils induced by Entamoeba histolytica, 2005, pp.174, 4279~4288
  5. "Gamma interferon induces Fas-dependent apoptosis of Peyer's patch T cells in mice following peroral infection with Toxoplasma gondii", 65, pp.4682~4689 (1997)
  6. "Fas-FasL interaction involved in pathogenesis of ocular toxoplasmosis in mice", 67, pp.928~935 (1999)
  7. "Mechanisms of apoptosis in murine fibroblasts by two intracellular protozoan parasites, Toxoplasma gondii and Neospora caninum", 24, pp.347~354 (2002)
  8. "IFN- overproduction and high level apoptosis are associated with high but not low virulence Toxoplasma gondii infection", 167, pp.902~909 (2001)
  9. "Toxoplasma gondii-infected cells are resistant to multiple inducers of apoptosis", 160, pp.1824~1830 (1998)
  10. "Caspase-3 activity and expression of Bcl-2 family in human neutrophils by Helicobacter pylori water-soluble proteins", 6, pp.207~215 (2001)
  11. "NF- B is a central regulator of the intestinal epithelial cell innate immune response induced by infection with enteroinvasive bacteria", 163, pp.1457~1466 (1999)
  12. "Nuclear factor-kappa B plays a major role in the regulation of chemokine expression of HeLa cells in response to Toxoplasma gondii infection", 87, pp.758~763 (2001)
  13. "Trichomonas vaginalis inhibits proinflammatory cytokine production in macrophages by suppressing NF- B activation", 18, pp.177~185 (2004)
  14. "Apoptosis of human intestinal epithelial cells after bacterial invasion", 102, pp.1815~1823 (1998)
  15. "Invasion by Toxoplasma gondii protects human-derived HL-60 cells from actinomycin D-induced apoptosis", 187, pp.221~226 (1999)
  16. "Inhibition of host cell apoptosis by Toxoplasma gondii is accompanied by reduced activation of the caspase cascade and alterations of poly (ADP-ribose) polymerase expression", 114, pp.3495~3505 (2001)
  17. "Inhibition of apoptosis by intracellular protozoan parasites", 31, pp.1166~1176 (2001)
  18. "Activation of NF-kappaB by Toxoplasma gondii correlates with increased expression of antiapoptotic genes and localization of phosphorylated IkappaB to the parasitophorous vacuole membrane", 116, pp.4359~4371 (2003)
  19. "Manipulation of mitogen-activated protein kinase", nuclear factor- kappa B-signaling cascades during intracellular Toxoplasma gondii infection, 2004, pp.201, 191~205
  20. "Toxoplasma gondii inhibits ultraviolet light-induced apoptosis through multiple interactions with the mitochondrion-dependent programmed cell death pathway", 8, pp.301~315 (2006)
  21. "Mechanisms underlying the manipulation of host apoptotic pathways by Toxoplasma gondii", 34, pp.381~391 (2004)
  22. "Manipulation of apoptosis in the host-parasite interaction", 20, pp.280~287 (2004)

관련 논문AI추천

Influence of calcium ion on host cell invasion and intracellular replication by Toxoplasma gondii류재숙, 민득영, 송현욱, 안명희, 이영하, 주경환대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2004 · 제42권 제4호Proliferation of Toxoplasma gondii Suppresses Host Cell Autophagy류재숙, 송현욱, 안명희, 이영하, 이윤진대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2013 · 제51권 제3호Interactions between secreted GRA proteins and host cell proteins across the parasitophorous vacuolar membrane in the parasitism of Toxoplasma gondii김희은, 남호우, 안혜진, Sehra Kim대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2006 · 제44권 제4호Effects of iNOS inhibitor on IFN-gamma production and apoptosis of splenocytes in genetically different strains of mice infected with Toxoplasma gondii강기만, 신대환, 이계성, 이영하, 이재호, 최인욱대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2004 · 제42권 제4호Involvement of MAPK activation in chemokine or COX-2 productions by Toxoplasma gondii김지영, 류재숙, 민득영, 송현욱, 안명희, 조명환, 최종학대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2006 · 제44권 제3호Protease activity of 80 kDa protein secreted from the apicomplexan parasite Toxoplasma gondiiHo-WooNAM, Kyoung-JuSONG대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2003 · 제41권 제3호

이 간행물의 다른 논문 (13건)

Kawasaki Disease김동수pp. 759-772Cytokeratin Autoantibodies: Useful Serologic Markers for Toluene Diisocyanate-Induced Asthma남동호, 김철우, 서창희, 예영민, 홍천수, 박춘식, 김형률, 박해심pp. 773-781Urachal Anomalies in Children: A Single Center Experience최윤정, 김종민, 오정탁, 안선영, 한상원, 이재승pp. 782-786Lack of Association between Polymorphisms of the Dopamine Receptor D4 and Dopamine Transporter Genes and Personality Traits in a Korean Population김찬형, 이홍식, 김영신, 김세주pp. 787-792The CobraPLATM During Anesthesia with Controlled Ventilation: A Clinical Trial of Efficacy이종석, 김민수, 유영철, 이윤우, 한동우, 남상범, 심연희pp. 799-804
원문 보기